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The Detection of Mutational Changes in Sorghum using RAPD

Indonesian Journal of Biotechnology · Vol. 16 No. 1 (2011)

DOI
10.22146/ijbiotech.7828
ISSN
2089-2241, 0853-8654
Terbit
2011-06-08
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

Sorghum is a multifunction plant due to its high economic value as a source of food, feed and industrial raw material of biofuel. Sorghum improvement can be done through mutation breeding and this research was conducted to evaluate the power of mutation breeding by observing the difference between mutant and its original counterpart. Varieties of sorghum Durra, Zhengzu and their mutants B-100 and Zh-30 were arranged in completely randomized design. DNA extraction was done using a modified CTAB method. After purification, quantification, and dilution, PCR was carried out for RAPD analysis. The result showed that Durra and Zhengzu varieties were significantly different in plant height, number of leaves and seed colour, however the mutant and its original counterpart cannot be differentiated morphologically. RAPD can be used to differentiate mutant and its original counterpart by observing the specific band pattern from each primer.

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  • Universitas Gadjah Mada
  • IPB University
  • Muhammadiyah University of Surakarta
  • Cenderawasih University
  • University of Brawijaya
  • Universitas Bangun Nusantara
  • Universitas Slamet Riyadi
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Rekan penulisOrganisasiArtikel bersama
Rudi Hari MurtiUniversitas Gadjah Mada3
Panjisakti BasunandaUniversitas Gadjah Mada3
Sugiharto Budi SantosoMuhammadiyah University of Surakarta2
Ratna Dewi EskundariUniversitas Bangun Nusantara2
Didik IndradewaUniversitas Gadjah Mada2
Yekti Asih PurwestriUniversitas Gadjah Mada2
A. RimbawantoAfiliasi tidak diketahui2
Yunus Pajanjan PaulanganCenderawasih University1
Muh. Arsyad Al. AminIPB University1
Barnabas BarapadangCenderawasih University1
Yudi WahyudinIPB University1
Rani Agustina WulandariUniversitas Gadjah Mada1
Vina Eka AristyaUniversitas Gadjah Mada1
Eko HanudinUniversitas Gadjah Mada1
Dewi Ratna NurhayatiUniversitas Slamet Riyadi1
Prapto YudonoUniversitas Gadjah Mada1
Yuli PriyanaMuhammadiyah University of Surakarta1
Suyadi SuyadiUniversity of Brawijaya1
Titin SetyoriniAfiliasi tidak diketahui1
Sapto IndriokoUniversitas Gadjah Mada1
K. KristamtiniAfiliasi tidak diketahui1
Kristamtini KristamtiniNational Research and Innovation Agency1
Yudi Agus SetiawanIPB University1
Widiatmaka WidiatmakaIPB University1
Wiwin AmbarwulanIPB University1
Hefni EffendiIPB University1
Muhamad Yanuar Jarwadi PurwantoIPB University1
Hamirul HadiniUniversitas Halu Oleo1
Nasrullah NasrullahAfiliasi tidak diketahui1
Panjisakti BasunandaAfiliasi tidak diketahui1
Sukartini SukartiniAfiliasi tidak diketahui1
Sismindari SismindariAfiliasi tidak diketahui1
Aminatun MunawartiUniversity of Brawijaya1
Endang SemiartiAfiliasi tidak diketahui1
Arif SatriaIPB University1
Rilus A. KinsengIPB University1
Fevi Rahma Dwi PutriIPB University1
Elisa AnggraeniIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">The Detection of Mutational Changes in Sorghum using RAPD</dc:title>
	<dc:creator>Taryono, T.</dc:creator>
	<dc:creator>Cahyaningrum, Paramita</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">Sorghum is a multifunction plant due to its high economic value as a source of food, feed and industrial raw material of biofuel. Sorghum improvement can be done through mutation breeding and this research was conducted to evaluate the power of mutation breeding by observing the difference between mutant and its original counterpart. Varieties of sorghum Durra, Zhengzu and their mutants B-100 and Zh-30 were arranged in completely randomized design. DNA extraction was done using a modified CTAB method. After purification, quantification, and dilution, PCR was carried out for RAPD analysis. The result showed that Durra and Zhengzu varieties were significantly different in plant height, number of leaves and seed colour, however the mutant and its original counterpart cannot be differentiated morphologically. RAPD can be used to differentiate mutant and its original counterpart by observing the specific band pattern from each primer.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2011-06-08</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7828</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7828</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 16, No 1 (2011)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7828/6069</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>