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Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon

Indonesian Journal of Biotechnology · Vol. 16 No. 2 (2011)

DOI
10.22146/ijbiotech.7837
ISSN
2089-2241, 0853-8654
Terbit
2011-12-06
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers. Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5

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Purnomo PurnomoAfiliasi tidak diketahui7
Arif muazamAfiliasi tidak diketahui3
Setyorini WidyayantiAfiliasi tidak diketahui3
Adib Fakhruddin YusufAfiliasi tidak diketahui3
Tuty ArisuryantiAfiliasi tidak diketahui3
Niken Satuti Nur HandayaniAfiliasi tidak diketahui3
KEIKO T. NATSUAKIAfiliasi tidak diketahui2
Endah RetnaningrumAfiliasi tidak diketahui2
Laila HanumSriwijaya University2
S. SantosaAfiliasi tidak diketahui2
R. RugayahAfiliasi tidak diketahui2
Fitri NadifahAfiliasi tidak diketahui2
Eko Agus SuyonoAfiliasi tidak diketahui2
Vida Rahma LatifahAfiliasi tidak diketahui2
Vivi Indah NurcahyatiAfiliasi tidak diketahui2
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Yekti Asih PurwestriAfiliasi tidak diketahui2
Muhammad Fauzi ArifAfiliasi tidak diketahui1
Azizah Tyas NugrahantyAfiliasi tidak diketahui1
Lucia Arum Sekar MeysariAfiliasi tidak diketahui1
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Hong-Wei YenAfiliasi tidak diketahui1
Laila Uswatun ChasanahAfiliasi tidak diketahui1
Suharyanto SuharyantoUniversitas Gadjah Mada1
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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon</dc:title>
	<dc:creator>Daryono, Budi Setiadi</dc:creator>
	<dc:creator>Aristya, Ganies Riza</dc:creator>
	<dc:creator>Kasiamdari, Rina Sri</dc:creator>
	<dc:description xml:lang="en-US">A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.    Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:date>2011-12-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7837</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7837</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 16, No 2 (2011)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7837/6078</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>