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Cloning and Expression of ORF124 Koi Herpesvirus as a Vaccine

Indonesian Journal of Biotechnology · Vol. 17 No. 1 (2012)

DOI
10.22146/ijbiotech.7850
ISSN
2089-2241, 0853-8654
Terbit
2012-06-03
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

Koi herpesvirus (KHV) which also known as Cyprinid herpesvirus 3 (CyHV-3), Koi herpes-like virus,and carp interstitial nephritis gill necrosis virus (CNGV), caused signifi cant morbidity and mortality in koiand common carp (Cyprinus carpio). The case fatality rate of this disease is 80–100%. Glycoprotein has beenused for vaccine development as sub unit vaccine against viruses. The aim of this research was to clone andexpress membrane glycoprotein ORF124 KHV as a candidate of recombinant vaccine. ORF124 KHV gene wassuccessfully cloned into pBSKS and sequenced. Result showed that ORF124 KHV (isolate from Indonesia) had100 % similarity with Cyprinid herpesvirus 3 strain TUMST1 (from Japan), 99% similarity with Koi herpesvirus strain KHV-U (from USA) and Koi herpesvirus strain KHV-I (from Israel). Prediction analysis of T and B cellepitopes showed that ORF124 KHV protein had 14 and 11 T cell epitopes (IAd, Rothbard/Taylor pattern),and had 10 B cell epitopes, suggested that the protein can be used as a vaccine candidate. ORF124 gene hasbeen expressed in Escherichia coli under pET32-a(+)vector.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Jenderal Soedirman University
  • Andalas University
  • Nara Institute of Science and Technology
  • University of Mataram
  • Universitas Al-Azhar Indonesia
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Rumiyati RumiyatiUniversitas Gadjah Mada3
Firman AlamsyahUniversitas Al-Azhar Indonesia3
Yekti Asih PurwestriUniversitas Gadjah Mada3
Edy MeiyantoUniversitas Gadjah Mada2
PUDJI ASTUTIAfiliasi tidak diketahui2
Langkah SembiringUniversitas Gadjah Mada2
Alfi Rizqi AmaliaUniversitas Gadjah Mada2
Woro Anindito Sri TunjungUniversitas Gadjah Mada2
Chio OkaNara Institute of Science and Technology2
Christina Retna HandayaniMinistry of Marine Affairs and Fisheries2
Taufik Budi PramonoAfiliasi tidak diketahui1
Norman Arie PrayogoJenderal Soedirman University1
Asrul Sahri SiregarJenderal Soedirman University1
Purnama SukardiJenderal Soedirman University1
Rita MalizaAndalas University1
Putra SantosoAndalas University1
Bramadi AryaMahidol University1
Alimuddin TofrizalAndalas University1
Suwarno HadisusantoUniversitas Gadjah Mada1
Sari Budi Moria SembiringNational Research and Innovation Agency1
Jhon Harianto HutapeaNational Research and Innovation Agency1
I Nyoman Adiasmara GiriNational Research and Innovation Agency1
Ketut MahardikaNational Research and Innovation Agency1
HaryantiNational Research and Innovation Agency1
Muhammad Da’iAfiliasi tidak diketahui1
Umar Anggara JenieUniversitas Gadjah Mada1
Supardjan AmAfiliasi tidak diketahui1
Jaka WidadaUniversitas Gadjah Mada1
Yani Lestari NurainiAfiliasi tidak diketahui1
Asmarani KusumawatiAfiliasi tidak diketahui1
Penny Humaidah HamidAfiliasi tidak diketahui1
Widya AsmaraUniversitas Gadjah Mada1
Wayan Tunas ArtamaUniversitas Gadjah Mada1
Sri DarmawatiUniversitas Muhammadiyah Semarang1
R. RumiyatiUniversitas Gadjah Mada1
Nunuk Aries NurulitaMuhammadiyah University Purwokerto1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Cloning and Expression of ORF124 Koi Herpesvirus as a Vaccine</dc:title>
	<dc:creator>Murwantoko, M.</dc:creator>
	<dc:creator>Setyowati, Dewi Nur'aeni</dc:creator>
	<dc:creator>Pratiwi, Rarastoeti</dc:creator>
	<dc:creator>Kawaichi, Masashi</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">  Koi herpesvirus (KHV) which also known as Cyprinid herpesvirus 3 (CyHV-3), Koi herpes-like virus,and carp interstitial nephritis gill necrosis virus (CNGV), caused signifi cant morbidity and mortality in koiand common carp (Cyprinus carpio). The case fatality rate of this disease is 80–100%. Glycoprotein has beenused for vaccine development as sub unit vaccine against viruses. The aim of this research was to clone andexpress membrane glycoprotein ORF124 KHV as a candidate of recombinant vaccine. ORF124 KHV gene wassuccessfully cloned into pBSKS and sequenced. Result showed that ORF124 KHV (isolate from Indonesia) had100 % similarity with Cyprinid herpesvirus 3 strain TUMST1 (from Japan), 99% similarity with Koi herpesvirus strain KHV-U (from USA) and Koi herpesvirus strain KHV-I (from Israel). Prediction analysis of T and B cellepitopes showed that ORF124 KHV protein had 14 and 11 T cell epitopes (IAd, Rothbard/Taylor pattern),and had 10 B cell epitopes, suggested that the protein can be used as a vaccine candidate. ORF124 gene hasbeen expressed in Escherichia coli under pET32-a(+)vector.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2012-06-03</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/force-download</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7850</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7850</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 17, No 1 (2012)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7850/6091</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
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