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Partial Purifi cation, Stability Analysis, and Preservation of Xylanase from Xylanolytic Alkalophylic Bacteria

Indonesian Journal of Biotechnology · Vol. 18 No. 1 (2013) · hlm. 1–7

DOI
10.22146/ijbiotech.7861
ISSN
2089-2241, 0853-8654
Terbit
2013-06-09
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

A xylanase, which produces xylose from oat spelt xylans, was isolated from the culture medium of xylanolytic alkalophylic bacteria mutant. The enzyme was purifi ed by ammonium sulphate with level 30, 40, 50, 60, 70, 80, and 90%. The purify of the fi nal preparation was demonstrated by sodium dodecyl sulphatepolyacrylamide gel electrophoresis. The molecular masses of the purifi ed xylanase were 137.61 and 165.34 kDa. Result of ammonium sulphate saturation with the highest activity was used as standart for saturation for enzyme production and preservation, using corn, tapioca, soy bean meal and gaplek fl our as carriers. Addition of 60% ammonium sulphate showed the highest xylanase activity (62.03 U/g), and produced 89.40% enzyme recovery. Tapioca, as a carrier, produced the highest xylanase activity. Key words: preservation, purifi cation, stability analysis, xylanase.

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  • Universitas Gadjah Mada
  • Universitas Muhammadiyah Purworejo
  • IPB University
  • Indonesian Institute of Sciences
  • National Research and Innovation Agency
  • Politeknik Pembangunan Pertanian Malang
  • Universitas Bangun Nusantara
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Rekan penulisOrganisasiArtikel bersama
Budi Prasetyo WidyobrotoUniversitas Gadjah Mada2
Dewi Ratih Ayu DaningPoliteknik Pembangunan Pertanian Malang2
Setiyono SetiyonoUniversitas Gadjah Mada1
Roisu Eny MudawarochUniversitas Muhammadiyah Purworejo1
Edi SuryantoUniversitas Gadjah Mada1
Widodo WidodoIPB University1
Asih KurniawatiUniversitas Gadjah Mada1
Wayan Tunas ArtamaUniversitas Gadjah Mada1
Ema DamayantiIndonesian Institute of Sciences1
Achmad DinotoNational Research and Innovation Agency1
Ahimsa Kandi SaririUniversitas Bangun Nusantara1
Kustantinah KustantinahUniversitas Gadjah Mada1
Zaenal BachruddinUniversitas Gadjah Mada1
Cahya Setya UtamaDiponegoro University1
Zuprizal ZuprizalUniversitas Gadjah Mada1
Wihandoyo WihandoyoUniversitas Gadjah Mada1
Nafiatul UmamiUniversitas Gadjah Mada1
Adib Norma RespatiPoliteknik Negeri Jember1
Agung IrawanSebelas Maret University1
Lukita PurnamayantiUniversitas Gadjah Mada1
Jamhari JamhariUniversitas Gadjah Mada1
Siti ZubaidahState University of Malang1
Zuprizal .Afiliasi tidak diketahui1
Bambang AriyadiUniversitas Gadjah Mada1
Aji Praba BaskaraUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Partial Purifi cation, Stability Analysis, and Preservation of Xylanase from Xylanolytic Alkalophylic Bacteria</dc:title>
	<dc:creator>Hanim, Chusnul</dc:creator>
	<dc:creator>Cahyanto, Muhamad Nur</dc:creator>
	<dc:creator>Yusiati, Lies Mira</dc:creator>
	<dc:creator>Wibowo, Ali</dc:creator>
	<dc:subject xml:lang="en-US">Preservation; Purification; Stability analysis; Xylanase</dc:subject>
	<dc:description xml:lang="en-US">A xylanase, which produces xylose from oat spelt xylans, was isolated from the culture medium of  xylanolytic alkalophylic bacteria mutant. The enzyme was purifi ed by ammonium sulphate with level 30, 40, 50, 60, 70, 80, and 90%. The purify of the fi nal preparation was demonstrated by sodium dodecyl sulphatepolyacrylamide gel electrophoresis. The molecular masses of the purifi ed xylanase were 137.61 and 165.34 kDa. Result of ammonium sulphate saturation with the highest activity was used as standart for saturation for enzyme production and preservation, using corn, tapioca, soy bean meal and gaplek fl our as carriers. Addition of 60% ammonium sulphate showed the highest xylanase activity (62.03 U/g), and produced 89.40% enzyme recovery. Tapioca, as a carrier, produced the highest xylanase activity. Key words: preservation, purifi cation, stability analysis, xylanase.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2013-06-09</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7861</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7861</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 18, No 1 (2013); 1-7</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7861/6102</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>