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The Application of Polymerase Chain Reaction – Restriction Fragment Polymorphisms (PCR-RFLP) to Determine Genetic Diversity of Madura Cattle in Sapudi Island

Indonesian Journal of Biotechnology · Vol. 18 No. 1 (2013) · hlm. 70–74

DOI
10.22146/ijbiotech.7869
ISSN
2089-2241, 0853-8654
Terbit
2013-06-09
Penerbit
Universitas Gadjah Mada
Bahasa
eng

Abstrak

The aim of this study was to determine genetic diversity of Madura cattle using Polymerase Chain Reaction – Restriction Fragment Length Polymorphisms (PCR-RFLP) analysis of the cytochrome b (cytb) gene. Samples used for the experiments were blood of 43 cattle that consist of 15 cattle obtained from Madura Island, 23 cattle from Sapudi Island, and 5 Limousin-Madura (Limura) cattle. A fragment of 464 base pair of cytb gene was amplifi ed by forward primer L14735 and reverse primer H15149. The PCR product was digested with TaqIand HinfI restriction enzymes to identify genetic patterns. Data of PCR-RFLP showed two haplotypes, that were A and B, in cattle obtained from both Madura Island and Sapudi Island. The frequencies of haplotype A and B of cattle from Sapudi Island were 69.57% and 30.47%, respectively. More diverse frequencies were observed in cattle obtained from Madura Island, where haplotype A and B were 86.67% and 13.33%, respectively. In this experiment, Limura cattle had only haplotype A. As a conclusion, PCR-RFLP of the cytb gene had been able to determine a genetic diversity of Madura cattle. Key words: Genetic diversity, Madura cattle, haplotype.

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  • Universitas Gadjah Mada
  • IPB University
  • Indonesian Institute of Sciences
  • Universitas Halu Oleo
  • Padjadjaran University
  • Sebelas Maret University
  • Uppsala University
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Rekan penulisOrganisasiArtikel bersama
Sri Hendrastuti HidayatIPB University3
Asmar HasanUniversitas Halu Oleo2
Kikin Hamzah MutaqinIPB University2
Asih KurniawatiUniversitas Gadjah Mada2
Endang Tri MargawatiIndonesian Institute of Sciences2
Indriawati IndriawatiIndonesian Institute of Sciences2
Widya AsmaraUniversitas Gadjah Mada2
Tiyas Tono TaufiqAfiliasi tidak diketahui2
Sumadi SumadiPadjadjaran University2
Suryo WiyonoIPB University2
Dyah MaharaniUniversitas Gadjah Mada2
KustantinahAfiliasi tidak diketahui2
Sigit BintaraUniversitas Gadjah Mada2
Richi Yuliavian KusminantoSebelas Maret University2
Muhammad TaufikUniversitas Halu Oleo1
Muhammad TaufikUniversitas Halu Oleo1
Sudarsono SudarsonoIPB University1
Fetrina OktaviaAfiliasi tidak diketahui1
Kuswanhadi KuswanhadiAfiliasi tidak diketahui1
Dini DinartyIPB University1
Lies Mira YusiatiUniversitas Gadjah Mada1
Wayan Tunas ArtamaUniversitas Gadjah Mada1
Emma SvenssonUppsala University1
Ety AryatiUniversitas Gadjah Mada1
Tutik Dwi WahyuningsihUniversitas Gadjah Mada1
Nosa Septiana AninditaAfiliasi tidak diketahui1
Sri LestariUniversitas Gadjah Mada1
Resti Yuliana RahmawatiUniversitas Gadjah Mada1
Yekti Asih PurwestriUniversitas Gadjah Mada1
Donny WidiantoUniversitas Gadjah Mada1
Yuni SineUniversitas Gadjah Mada1
Byong H. LeeMcGill University1
Sudirman YahyaIPB University1
Tri Rapani FebbiyantiPusat Penelitian Karet Indonesia1
Indira RiastiwiIndonesian Institute of Sciences1
Yashanti Berlinda ParadisaIndonesian Institute of Sciences1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">The Application of Polymerase Chain Reaction – Restriction Fragment Polymorphisms (PCR-RFLP) to Determine Genetic Diversity of Madura Cattle in Sapudi Island</dc:title>
	<dc:creator>Hartatik, Tety</dc:creator>
	<dc:creator>Volkandari, Slamet Diah</dc:creator>
	<dc:creator>Sumadi, S.</dc:creator>
	<dc:creator>Widodo, W.</dc:creator>
	<dc:subject xml:lang="en-US">Genetic diversity; Madura cattle; Haplotype</dc:subject>
	<dc:description xml:lang="en-US">The aim of this study was to determine genetic diversity of Madura cattle using Polymerase Chain Reaction – Restriction Fragment Length Polymorphisms (PCR-RFLP) analysis of the cytochrome b (cytb) gene. Samples used for the experiments were blood of 43 cattle that consist of 15 cattle obtained from Madura Island, 23 cattle from Sapudi Island, and 5 Limousin-Madura (Limura) cattle. A fragment of 464 base pair of cytb gene was amplifi ed by forward primer L14735 and reverse primer H15149. The PCR product was digested with TaqIand HinfI restriction enzymes to identify genetic patterns. Data of PCR-RFLP showed two haplotypes, that were A and B, in cattle obtained from both Madura Island and Sapudi Island. The frequencies of haplotype A and B of cattle from Sapudi Island were 69.57% and 30.47%, respectively. More diverse frequencies were observed in cattle obtained from Madura Island, where haplotype A and B were 86.67% and 13.33%, respectively. In this experiment, Limura cattle had only haplotype A. As a conclusion, PCR-RFLP of the cytb gene had been able to determine a genetic diversity of Madura cattle. Key words: Genetic diversity, Madura cattle, haplotype.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2013-06-09</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7869</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7869</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 18, No 1 (2013); 70-74</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7869/6110</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>