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Cytotoxicity of Buah Merah (Pandanus conoideus Lamk.) Extract on Breast Cancer Cell Line (T47D)

Indonesian Journal of Biotechnology · Vol. 19 No. 1 (2014) · hlm. 71–78

DOI
10.22146/ijbiotech.8636
ISSN
2089-2241, 0853-8654
Terbit
2014-06-05
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Buah Merah (Pandanus conoideus Lamk.) has been extensively used to treat various diseases includingcancer. There are many varieties of buah merah and there was no scientifi c study comparing cytotoxicity ofdifferent varieties. The objective of this study was to investigate the cytotoxicity of three varieties of buah merahknown as Barugum, Maler and Yanggiru on breast cancer cell line (T47D). All samples were collected fromPapua, Indonesia. Each sample was extracted consecutively using three solvents chloroform, methanol andwater resulted to nine crude extracts. The cytotoxic activities were determined using MTT assay. The crudeextract showed the lowest IC50 was selected for further bioassay-guided fractionation. Fractionation was doneusing vacuum liquid chromatography coupled with preparative TLC to fi nd the active compounds. Severaldetection reagents were applied to TLC for identifi cation of the class of the potent compounds. The resultshowed that the potent extracts was obtained from Barugum methanol extract followed by Maler chloroformextract with IC50 value of 132.83 μg/ml and 139.72 μg/ml, respectively. All Yanggiru extracts did not showactivity. The bioassay-guided fractionation of Barugum and Maler extracts showed that the most potent fractioneluted by a mixture of hexane:ethyl acetate (75:25), was in Maler variety with IC50 value of 25,7 μg/ml, fourtimes higher than the most potent fraction of Barugum with IC50 value of 104,61 μg/ml. TLC analysis of themost potent fraction showed that the active compounds was class of terpene. Result of this study supportedthe utilization of buah merah Maler variety for breast cancer treatment.

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  • University of Brawijaya
  • State University of Semarang
  • Universitas Trisakti
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Rekan penulisOrganisasiArtikel bersama
Endang SemiartiAfiliasi tidak diketahui6
Aziz PurwantoroAfiliasi tidak diketahui5
Puji AstutiAfiliasi tidak diketahui2
Syarif HidayatAfiliasi tidak diketahui2
Nintya SetiariAfiliasi tidak diketahui2
Exsyupransia MursyantiAfiliasi tidak diketahui2
Imam CholissodinUniversity of Brawijaya1
Sisco JupiyandiUniversity of Brawijaya1
Fadhil Rizqullah SaniputraUniversity of Brawijaya1
Muhammad Robby DharmawanUniversity of Brawijaya1
Isa AkhlisState University of Semarang1
Suharto LinuwihAfiliasi tidak diketahui1
Ririn SuharsantiAfiliasi tidak diketahui1
Nunung YuniartiAfiliasi tidak diketahui1
Roro Meifi PitalokaAfiliasi tidak diketahui1
Calvin Dominggo HattuAfiliasi tidak diketahui1
Alia AfiyatiAfiliasi tidak diketahui1
Clara Indah NatashaAfiliasi tidak diketahui1
Eka YuniarAfiliasi tidak diketahui1
Faizal PikriAfiliasi tidak diketahui1
S. M. WidyastutiAfiliasi tidak diketahui1
Etik Erna Wati HadiAfiliasi tidak diketahui1
Erma SulistyaningsihAfiliasi tidak diketahui1
Wayan T. ArtamaAfiliasi tidak diketahui1
Jarot SubandonoAfiliasi tidak diketahui1
Ixora Sartika MercurianiAfiliasi tidak diketahui1
Seonghoe JangAfiliasi tidak diketahui1
Pudji AstutiUniversitas Trisakti1
Laurentius Hartanto NugrohoAfiliasi tidak diketahui1
Retno AryaniAfiliasi tidak diketahui1
SEBASTIAN MARGINOAfiliasi tidak diketahui1
Sri YuwantiningsihAfiliasi tidak diketahui1
Sri WahyuningsihAfiliasi tidak diketahui1
Budi Setiadi DaryonoAfiliasi tidak diketahui1
Muhammad Dylan LawrieAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Cytotoxicity of Buah Merah (Pandanus conoideus Lamk.) Extract on Breast Cancer Cell Line (T47D)</dc:title>
	<dc:creator>Nuringtyas, Tri R.</dc:creator>
	<dc:creator>Pratama, Yoga</dc:creator>
	<dc:creator>Galih, G.</dc:creator>
	<dc:creator>Wahyuono, Subagus</dc:creator>
	<dc:creator>Moeljopawiro, Sukarti</dc:creator>
	<dc:subject xml:lang="en-US">Buah Merah; cytotoxicity; T47D; vacuum liquid chromatography; thin-layer chromatography</dc:subject>
	<dc:description xml:lang="en-US">Buah Merah (Pandanus conoideus Lamk.) has been extensively used to treat various diseases includingcancer. There are many varieties of buah merah and there was no scientifi c study comparing cytotoxicity ofdifferent varieties. The objective of this study was to investigate the cytotoxicity of three varieties of buah merahknown as Barugum, Maler and Yanggiru on breast cancer cell line (T47D). All samples were collected fromPapua, Indonesia. Each sample was extracted consecutively using three solvents chloroform, methanol andwater resulted to nine crude extracts. The cytotoxic activities were determined using MTT assay. The crudeextract showed the lowest IC50 was selected for further bioassay-guided fractionation. Fractionation was doneusing vacuum liquid chromatography coupled with preparative TLC to fi nd the active compounds. Severaldetection reagents were applied to TLC for identifi cation of the class of the potent compounds. The resultshowed that the potent extracts was obtained from Barugum methanol extract followed by Maler chloroformextract with IC50 value of 132.83 μg/ml and 139.72 μg/ml, respectively. All Yanggiru extracts did not showactivity. The bioassay-guided fractionation of Barugum and Maler extracts showed that the most potent fractioneluted by a mixture of hexane:ethyl acetate (75:25), was in Maler variety with IC50 value of 25,7 μg/ml, fourtimes higher than the most potent fraction of Barugum with IC50 value of 104,61 μg/ml. TLC analysis of themost potent fraction showed that the active compounds was class of terpene. Result of this study supportedthe utilization of buah merah Maler variety for breast cancer treatment.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2014-06-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/8636</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.8636</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 19, No 1 (2014); 71-78</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/8636/6623</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Tri R Nuringtyas, Yoga Pratama, Galih G, Subagus Wahyuono, Sukarti Moeljopawiro</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>