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In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection

Indonesian Journal of Biotechnology · Vol. 19 No. 2 (2014) · hlm. 111–120

DOI
10.22146/ijbiotech.9301
ISSN
2089-2241, 0853-8654
Terbit
2014-12-08
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

In vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds fromtwo different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material.Double concentration of MS (Murashige & Skoog, 1962) with 40 mg/l gall rust (Uromycladium tepperianum(Sacc.) fi ltrate were used for media selection. The results of this research showed that 66 % axenic plantlets invitro from S1 and 27 % from S2 were still survived after 3 months incubation without subculture. The meanof fresh weight (2. 21 ± 0. 26 g) and dry weight (1. 97 ± 0. 12 g) from S1 plantlets lower than the mean of freshweight (2. 87 ± 0,18 g) and dry weight (2. 16 ± 0. 14 g) from S2 plantlets. Qualitative of terpenes, saponins andquantitative of total phenolics were analyzed from those gall rust extract, as source of fi ltrate media, attackedand un-attacked of F. moluccana. They all qualitatively have capability to produce terpenoid and saponin. Itis notice that U. tepperianum, un-cultured pathogen, contain of those compound that may play a role as codeterminantsof pathogenecity. While the highest total phenolic compound were contained in gall rust extract(2. 35 %), followed by attacked F. moluccana branches (1. 18 %) and un-attacked F. moluccana branches (0. 44%). This indicated that phenolic compound in gall rust has higher activity as a response of F. moluccana to U.tepperianum pathogen pressures and result of this study suggest the great value of gall rust fi ltrate for use asmedia selection in vitro.

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  • Universitas Gadjah Mada
  • IPB University
  • Universitas Jambi
  • University of Brawijaya
  • Hasanuddin University
  • Universitas Nahdlatul Ulama Lampung
  • Universitas Halu Oleo
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Yudi YudiUniversitas Jambi4
Endang SemiartiUniversitas Gadjah Mada4
Rahayu RahayuUniversitas Jambi3
Dheny WahyudhiUniversitas Jambi3
Aziz PurwantoroUniversitas Gadjah Mada3
Muhaimin Rifa’iUniversity of Brawijaya3
Sri WidyartiAfiliasi tidak diketahui3
Luki AbdullahIPB University2
Asnath Maria FuahAfiliasi tidak diketahui2
Herry LiyusUniversitas Jambi2
Aris SoewondoUniversity of Brawijaya2
Aries Bagus SasongkoUniversitas Gadjah Mada2
Cece SumantriIPB University1
Asep GunawanIPB University1
Ratna Sholatia HarahapIPB University1
Kasita ListyariniIPB University1
Salman YusufAfiliasi tidak diketahui1
Rachmat SomanjayaAfiliasi tidak diketahui1
Mohamad Agus SetiadiAfiliasi tidak diketahui1
Agung Prastyo NugrohoAfiliasi tidak diketahui1
Merryafinola IfaniAfiliasi tidak diketahui1
Suhartati SuhartatiAfiliasi tidak diketahui1
Anhar Faisal FananiUniversitas Nahdlatul Ulama Lampung1
Komang Gede WiryawanIPB University1
Salundik SalundikAfiliasi tidak diketahui1
La Ode Safiun ArihiUniversitas Halu Oleo1
Hikmawati HikmawatiAfiliasi tidak diketahui1
Irdam IrdamAfiliasi tidak diketahui1
Dani SetiawanAfiliasi tidak diketahui1
Fransiskus OdiAfiliasi tidak diketahui1
Hamrullah HamrullahAfiliasi tidak diketahui1
Andi Arya Setiawan JuniorAfiliasi tidak diketahui1
Rahmatia RahmatiaAfiliasi tidak diketahui1
Muh. Alwy ArifinHasanuddin University1
Arifin SewengHasanuddin University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection</dc:title>
	<dc:creator>Putri, Asri Insiana</dc:creator>
	<dc:creator>Na'iem, Mohammad</dc:creator>
	<dc:creator>Indrioko, Sapto</dc:creator>
	<dc:creator>Rahayu, Sri</dc:creator>
	<dc:creator>Indrianto, Ari</dc:creator>
	<dc:subject xml:lang="en-US">F. moluccana; U. tepperianum; gall rust; in vitro selection</dc:subject>
	<dc:description xml:lang="en-US">In vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds fromtwo different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material.Double concentration of MS (Murashige &amp; Skoog, 1962) with 40 mg/l gall rust (Uromycladium tepperianum(Sacc.) fi ltrate were used for media selection. The results of this research showed that 66 % axenic plantlets invitro from S1 and 27 % from S2 were still survived after 3 months incubation without subculture. The meanof fresh weight (2. 21 ± 0. 26 g) and dry weight (1. 97 ± 0. 12 g) from S1 plantlets lower than the mean of freshweight (2. 87 ± 0,18 g) and dry weight (2. 16 ± 0. 14 g) from S2 plantlets. Qualitative of terpenes, saponins andquantitative of total phenolics were analyzed from those gall rust extract, as source of fi ltrate media, attackedand un-attacked of F. moluccana. They all qualitatively have capability to produce terpenoid and saponin. Itis notice that U. tepperianum, un-cultured pathogen, contain of those compound that may play a role as codeterminantsof pathogenecity. While the highest total phenolic compound were contained in gall rust extract(2. 35 %), followed by attacked F. moluccana branches (1. 18 %) and un-attacked F. moluccana branches (0. 44%). This indicated that phenolic compound in gall rust has higher activity as a response of F. moluccana to U.tepperianum pathogen pressures and result of this study suggest the great value of gall rust fi ltrate for use asmedia selection in vitro.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2014-12-08</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/9301</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.9301</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 19, No 2 (2014); 111-120</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/9301/6896</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 Asri Insiana Putri, Mohammad Na'iem, Sapto Indrioko, Sri Rahayu, Ari Indrianto</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>