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The Use of DNA Microsatellite Markers for Genetic Diversity Identification of Soybean (Glycine max (L) Meriil.) as a Supplementary Method in Reference Collections Management

Indonesian Journal of Biotechnology · Vol. 19 No. 2 (2014) · hlm. 136–145

DOI
10.22146/ijbiotech.9306
ISSN
2089-2241, 0853-8654
Terbit
2014-12-08
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Large number of new soybean varieties are mostly derived from crosses of elite genotypes resulted ina narrowing of both the genetic diversity and the phylogenetic relationship between soybean varieties. Thus,discrimination among soybean varieties is becoming more diffi cult, especially when morphological traits wereapplied. In Plant Variety Protection (PVP) system, new varieties of soybeans including granted PVP right, localand breeding varieties registered in PVP offi ce were frequently increased, implicate on increasingly the numberof soybean varieties collections. To assist the management of varieties collections, a standard fi ngerprinting datais further needed. In comparison to the management of plant collection in the fi eld, molecular marker systemswhich are rapid, reliable, informative and relatively simple are continually sought for practical applications ingermplasm conservation, management and enhancement. This study aimed to identify the genetic diversity andphylogenetic relationship of soybean varieties that have earned PVP Right as well as local varieties and breedingvarieties registered in the PVP offi ce using microsatellite or simple sequence repeats (SSR) markers.This study was conducted in Molecular Biology laboratory, Indonesian Center for Agricultural Biotechnologyand Genetic Resources Research and Development (ICABIOGRAD) Bogor, from February to May 2013. The datawere analyzed using the genetic analysis package NTSYSpc 2.02i and PowerMarker V3.25. The result showed arelatively narrow genetic diversity among 45 varieties of soybean analyzed in present study which were indicatedby the small number of genotypes and total number of alleles (NA), and the low value of gene diversity and PICvalues (<0.75). Cluster analysis showed that the grouping varieties are not related to morphological characters butrelated to phylogeny relationship between varieties. Despite the group of varieties were not clustered in accordancewith morphological characteristics, SSR marker can be a powerful tool for discriminating varieties, so that it couldbe useful for initial varieties identity in conjunction with genetic diversity analysis.

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  • Sebelas Maret University
  • Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development
  • IPB University
  • National Research and Innovation Agency
  • State University of Semarang
  • National Nuclear Energy Agency of Indonesia
  • University of Indonesia
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  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Media Fitri Isma NugrahaAfiliasi tidak diketahui2
Hessy NovitaNational Research and Innovation Agency2
Rossa YunitaIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development2
Wening EnggariniIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development2
Yuliasti YuliastiNational Nuclear Energy Agency of Indonesia2
Getut PramestiSebelas Maret University1
Muh. Alias L. RajamuddinIPB University1
Fasya Hadaina MaharaniAfiliasi tidak diketahui1
Berna ElyaUniversity of Indonesia1
Atriyon JulzarikaIndonesian National Institute of Aeronautics and Space1
Alias RadjamuddinAfiliasi tidak diketahui1
Sucihatiningsih Dian Wisika PrajantiState University of Semarang1
Amin PujiatiState University of Semarang1
Agus PujiantoState University of Semarang1
Nandariyah NandariyahSebelas Maret University1
Suhadi Sapto YuwonoSebelas Maret University1
Mercy Bientri YunindanovaSebelas Maret University1
Sudarmi SudarmiAfiliasi tidak diketahui1
Samanhudi SamanhudiSebelas Maret University1
Nurmalita WaluyoAfiliasi tidak diketahui1
Iteu Margaret HidayatAfiliasi tidak diketahui1
Tri HandayaniIndonesian Institute of Sciences1
Ineu SulastriniAfiliasi tidak diketahui1
Salamiah SalamiahAfiliasi tidak diketahui1
Lelya PramudyaniAfiliasi tidak diketahui1
Hajrial AswidinnoorAfiliasi tidak diketahui1
Dwinita Wikan UtamiAfiliasi tidak diketahui1
SUGIONO MOELJOPAWIROAfiliasi tidak diketahui1
IDA HANARIDAAfiliasi tidak diketahui1
KusmanaNational Research and Innovation Agency1
SobirAfiliasi tidak diketahui1
Ika RoostikaNational Research and Innovation Agency1
Dwinita Wikan UtamiIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development1
Awang MaharijayaIPB University1
Usep JenudinMinistry of Agriculture1
Lutfi Tri AndrianiSebelas Maret University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">The Use of DNA Microsatellite Markers for Genetic Diversity Identification of Soybean (Glycine max (L) Meriil.) as a Supplementary Method in Reference Collections Management</dc:title>
	<dc:creator>Widaningsih, Nina Agusti</dc:creator>
	<dc:creator>Purwanto, Edi</dc:creator>
	<dc:creator>Nandariyah, N.</dc:creator>
	<dc:creator>Reflinur, R.</dc:creator>
	<dc:subject xml:lang="en-US">Soybean; microsatellites; genetic diversity; PVP</dc:subject>
	<dc:description xml:lang="en-US">Large number of new soybean varieties are mostly derived from crosses of elite genotypes resulted ina narrowing of both the genetic diversity and the phylogenetic relationship between soybean varieties. Thus,discrimination among soybean varieties is becoming more diffi cult, especially when morphological traits wereapplied. In Plant Variety Protection (PVP) system, new varieties of soybeans including granted PVP right, localand breeding varieties registered in PVP offi ce were frequently increased, implicate on increasingly the numberof soybean varieties collections. To assist the management of varieties collections, a standard fi ngerprinting datais further needed. In comparison to the management of plant collection in the fi eld, molecular marker systemswhich are rapid, reliable, informative and relatively simple are continually sought for practical applications ingermplasm conservation, management and enhancement. This study aimed to identify the genetic diversity andphylogenetic relationship of soybean varieties that have earned PVP Right as well as local varieties and breedingvarieties registered in the PVP offi ce using microsatellite or simple sequence repeats (SSR) markers.This study was conducted in Molecular Biology laboratory, Indonesian Center for Agricultural Biotechnologyand Genetic Resources Research and Development (ICABIOGRAD) Bogor, from February to May 2013. The datawere analyzed using the genetic analysis package NTSYSpc 2.02i and PowerMarker V3.25. The result showed arelatively narrow genetic diversity among 45 varieties of soybean analyzed in present study which were indicatedby the small number of genotypes and total number of alleles (NA), and the low value of gene diversity and PICvalues (&lt;0.75). Cluster analysis showed that the grouping varieties are not related to morphological characters butrelated to phylogeny relationship between varieties. Despite the group of varieties were not clustered in accordancewith morphological characteristics, SSR marker can be a powerful tool for discriminating varieties, so that it couldbe useful for initial varieties identity in conjunction with genetic diversity analysis.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2014-12-08</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/9306</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.9306</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 19, No 2 (2014); 136-145</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/9306/6899</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 Nina Agusti Widaningsih, Edi Purwanto, Nandariyah N, Reflinur R</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>