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Somatic embryogenesis of Sandalwood (Santalum album L.)

Indonesian Journal of Biotechnology · Vol. 19 No. 2 (2014) · hlm. 168–175

DOI
10.22146/ijbiotech.9311
ISSN
2089-2241, 0853-8654
Terbit
2014-12-08
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Sandalwood (Santalum album L.) is native species of Indonesia, especially in East Nusa Tenggara, is oneof the twenty two species of the genus Santalum in the world. Sandalwood is an important tree because it hashigh economic value can produce sandal oil these can be used for perfumes, cosmetics, pharmaceuticals, andare often used in religious ceremonies. In vitro particularly somatic embryogenesis has been widely appliedin the propagation of sandalwood. The Objective of this research is to obtain regeneration of sandalwoodthrough somatic embryogenesis using leaves explant from various clones. Medium for embryo induction is MS(Murashige and Skoog, 1962) solid medium containing treatment of 2,4-D (2,4-Dichlorophenoxyacetic acid)at various concentrations. To the media 0,15 mg /l kinetin, 40 g/l sucrose, and 2,5 g/l gelrite were added.Culture were incubated in the dark. Medium for Embryo development (maturation) is MS solid mediumcontaining treatment of BAP (Benzyl-amino-purine) at various concentrations. To the media 0,01 mg /l NAA(Napthalene-acetic-acid), 40 g/l sucrose, and 2,5 g/l gelrite were added. Culture were incubated in the light. Tostudy the specifi c structure of sandalwood somatic embryo early detection was conducted using histologicalanalysis. Results of anova showed that the clones, media, and interaction between clones with media did notsignifi cantly affect the development of sandalwood callus percentage. Results of anova showed that the clonesand BAP concentration signifi cantly effect to the embryo development of sandalwood.

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  • Universitas Gadjah Mada
  • Hasanuddin University
  • Universitas Jember
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Rekan penulisOrganisasiArtikel bersama
Endang SemiartiAfiliasi tidak diketahui4
Aziz PurwantoroAfiliasi tidak diketahui3
Sri RahayuHasanuddin University3
Asri Insiana PutriAfiliasi tidak diketahui3
Aries Bagus SasongkoUniversitas Gadjah Mada2
Windi MoseAfiliasi tidak diketahui1
Muhammad Dylan LawrieAfiliasi tidak diketahui1
Zulfa LayinaAfiliasi tidak diketahui1
Della Rosiana NingtiasAfiliasi tidak diketahui1
Falah Nur AlifiantoAfiliasi tidak diketahui1
T TaryonoAfiliasi tidak diketahui1
Titin SetyoriniAfiliasi tidak diketahui1
S. SuyadiAfiliasi tidak diketahui1
Tri Suwarni WahyudiningsihAfiliasi tidak diketahui1
Mohammad NaiemAfiliasi tidak diketahui1
Issirep SumardiAfiliasi tidak diketahui1
Eny FaridahAfiliasi tidak diketahui1
Yeni Widyana NurchahyaniAfiliasi tidak diketahui1
Atus SyahbudinAfiliasi tidak diketahui1
Jayusman JayusmanAfiliasi tidak diketahui1
Eko Bhakti HardiyantoAfiliasi tidak diketahui1
Muhammad Na’iemAfiliasi tidak diketahui1
ILG NurcahyaningsihAfiliasi tidak diketahui1
Asruwaidah FatumiAfiliasi tidak diketahui1
Oktaviana HerawatiAfiliasi tidak diketahui1
Asri Fajar MilasariAfiliasi tidak diketahui1
Maryani MaryaniUniversitas Jember1
Devi Bunga PagallaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Somatic embryogenesis of Sandalwood (Santalum album L.)</dc:title>
	<dc:creator>Herawan, Toni</dc:creator>
	<dc:creator>Na'iem, Mohammad</dc:creator>
	<dc:creator>Indrioko, Sapto</dc:creator>
	<dc:creator>Indrianto, Ari</dc:creator>
	<dc:subject xml:lang="en-US">Somatic embryogenesis, Santalum album, Embryogenic callus, maturation</dc:subject>
	<dc:description xml:lang="en-US">Sandalwood (Santalum album L.) is native species of Indonesia, especially in East Nusa Tenggara, is oneof the twenty two species of the genus Santalum in the world. Sandalwood is an important tree because it hashigh economic value can produce sandal oil these can be used for perfumes, cosmetics, pharmaceuticals, andare often used in religious ceremonies. In vitro particularly somatic embryogenesis has been widely appliedin the propagation of sandalwood. The Objective of this research is to obtain regeneration of sandalwoodthrough somatic embryogenesis using leaves explant from various clones. Medium for embryo induction is MS(Murashige and Skoog, 1962) solid medium containing treatment of 2,4-D (2,4-Dichlorophenoxyacetic acid)at various concentrations. To the media 0,15 mg /l kinetin, 40 g/l sucrose, and 2,5 g/l gelrite were added.Culture were incubated in the dark. Medium for Embryo development (maturation) is MS solid mediumcontaining treatment of BAP (Benzyl-amino-purine) at various concentrations. To the media 0,01 mg /l NAA(Napthalene-acetic-acid), 40 g/l sucrose, and 2,5 g/l gelrite were added. Culture were incubated in the light. Tostudy the specifi c structure of sandalwood somatic embryo early detection was conducted using histologicalanalysis. Results of anova showed that the clones, media, and interaction between clones with media did notsignifi cantly affect the development of sandalwood callus percentage. Results of anova showed that the clonesand BAP concentration signifi cantly effect to the embryo development of sandalwood.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2014-12-08</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/9311</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.9311</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 19, No 2 (2014); 168-175</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/9311/6903</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 Toni Herawan, Mohammad Na'iem, Sapto Indrioko, Ari Indrianto</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>