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Diversity of Nonribosomal Peptide Synthetase Genes in the AnticancerProducing Actinomycetes Isolated from Marine Sediment in Indonesia

Indonesian Journal of Biotechnology · Vol. 20 No. 1 (2015) · hlm. 34–41

DOI
10.22146/ijbiotech.15266
ISSN
2089-2241, 0853-8654
Terbit
2015-06-08
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Marine actinomycetes is a group of bacteria that is highly potential in producing novel bioactivecompound. It has unique characteristics and is different from other terrestrial ones. Extreme environmentalcondition is suspected to lead marine actinomycetes produce different types of bioactive compoundfound previously. The aim of this study was to explore the presence and diversity of NRPS genes in 14anticancer-producing actinomycetes isolated from marine sediment in Indonesia. PCR amplificationand restriction fragment analysis of NRPS genes with HaeIII from 14 marine actinomycetes were doneto assess the diversity of NRPS genes. Genome mining of one species of marine actinomycetes (strainGMY01) also was employed towards this goal. The result showed that NRPS gene sequence diversity in 14marine actinomycetes could be divided into 4 groups based on NRPS gene restriction patterns. Analysisof 16S rRNA gene sequences of representatives from each group showed that all isolates belong to genusof Streptomyces. Genome mining result showed that strain GMY01 harboring 10 different NRPS geneclusters that encode secondary metabolites, as pure NRPS or hybrid between NRPS and other compounds.These results indicated that marine actinomycetes having a high potential to be developed as source ofanticancer drugs development.

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  • Universitas Gadjah Mada
  • National Institute of Technology and Evaluation
  • Jenderal Soedirman University
  • Sebelas Maret University
  • The University of Tokyo
  • Justus-Liebig-Universität Gießen
  • IPB University
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Rekan penulisOrganisasiArtikel bersama
Aris Tri HaryantoUniversitas Gadjah Mada7
Tri WibawaUniversitas Gadjah Mada6
Michael KannJustus-Liebig-Universität Gießen3
Siti KabirunUniversitas Gadjah Mada3
Irfan Dwidya PrijambadaUniversitas Gadjah Mada3
Endang SemiartiUniversitas Gadjah Mada2
N. NgadimanUniversitas Gadjah Mada2
Reni NurjasmiAfiliasi tidak diketahui2
Widya AsmaraUniversitas Gadjah Mada2
Alimuddin AlimuddinIPB University2
Donny WidiantoUniversitas Gadjah Mada2
Sri WedhastriUniversitas Gadjah Mada2
Retno Sunarminingsih SudibyoUniversitas Gadjah Mada2
Hevi WihadmadyatamiUniversitas Gadjah Mada2
Diani MentariJenderal Soedirman University2
Edy MeiyantoUniversitas Gadjah Mada2
M. MurwantokoAfiliasi tidak diketahui2
Subagus WahyuonoUniversitas Gadjah Mada2
Rinawati RinawatiUniversitas Teuku Umar1
E. SuharyantoUniversitas Gadjah Mada1
Sudadi SudadiSebelas Maret University1
Sumarno SumarnoInstitut Teknologi Sepuluh Nopember1
Tri Joko RaharjoState University of Semarang1
Stalis Norma EthicaUniversitas Muhammadiyah Semarang1
Oedjijono OedjijonoJenderal Soedirman University1
Nur Fitriana AriyantiUniversitas Gadjah Mada1
Siti SubandiyahUniversitas Gadjah Mada1
Eka Tarwaca Susila PutraUniversitas Gadjah Mada1
Sukmana Siswandana PutraUniversitas Gadjah Mada1
Bambang Hendro SunarmintoUniversitas Gadjah Mada1
Mujiyo MujiyoSebelas Maret University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Diversity of Nonribosomal Peptide Synthetase Genes in the AnticancerProducing Actinomycetes Isolated from Marine Sediment in Indonesia</dc:title>
	<dc:creator>Herdini, Camelia</dc:creator>
	<dc:creator>Hartanto, Shinta</dc:creator>
	<dc:creator>Mubarika, Sofia</dc:creator>
	<dc:creator>Hariwiyanto, Bambang</dc:creator>
	<dc:creator>Wijayanti, Nastiti</dc:creator>
	<dc:creator>Hosoyama, Akira</dc:creator>
	<dc:creator>Yamazoe, Atsushi</dc:creator>
	<dc:creator>Nojiri, Hideaki</dc:creator>
	<dc:creator>Widada, Jaka</dc:creator>
	<dc:subject xml:lang="en-US">marine actinomycetes; non-ribosomal peptide synthetase genes diversity; Streptomyces; genome mining; anticancer drugs development</dc:subject>
	<dc:description xml:lang="en-US">Marine actinomycetes is a group of bacteria that is highly potential in producing novel bioactivecompound. It has unique characteristics and is different from other terrestrial ones. Extreme environmentalcondition is suspected to lead marine actinomycetes produce different types of bioactive compoundfound previously. The aim of this study was to explore the presence and diversity of NRPS genes in 14anticancer-producing actinomycetes isolated from marine sediment in Indonesia. PCR amplificationand restriction fragment analysis of NRPS genes with HaeIII from 14 marine actinomycetes were doneto assess the diversity of NRPS genes. Genome mining of one species of marine actinomycetes (strainGMY01) also was employed towards this goal. The result showed that NRPS gene sequence diversity in 14marine actinomycetes could be divided into 4 groups based on NRPS gene restriction patterns. Analysisof 16S rRNA gene sequences of representatives from each group showed that all isolates belong to genusof Streptomyces. Genome mining result showed that strain GMY01 harboring 10 different NRPS geneclusters that encode secondary metabolites, as pure NRPS or hybrid between NRPS and other compounds.These results indicated that marine actinomycetes having a high potential to be developed as source ofanticancer drugs development.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-06-08</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/15266</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.15266</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 20, No 1 (2015); 34-41</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/15266/10248</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 Camelia Herdini, Shinta Hartanto, Sofia Mubarika, Bambang Hariwiyanto, Nastiti Wijayanti, Akira Hosoyama, Atsushi Yamazoe, Hideaki Nojiri, Jaka Widada</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>