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High Resolution Microsatellite Marker Analysis of Some Rice Landraces Using Metaphor Agarose Gel Electrophoresis

Indonesian Journal of Biotechnology · Vol. 20 No. 1 (2015) · hlm. 54–61

DOI
10.22146/ijbiotech.15269
ISSN
2089-2241, 0853-8654
Terbit
2015-06-08
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Microsatellite markers or simple sequences repeats are DNA - based molecular techniques that areused to see the different among accessions and inbred lines. There are three methods to analysis the results ofthe polymerase chain reaction of microsatellite markers namely polyacrylamide gel electrophoresis (PAGE),capillary electroforesis, and Metaphor Agarose Gel Electroforesis (MAGE), and the Use of MAGE assessedmore easily and economically the polymorphic pattern of DNA markers. This study aimed to obtain fast,effective and efficient in term of easy and cheap technique to identify microsatellite markers of some blackrice cultivars and F2 populations from crosses between black with white rice. The results showed that MAGEsuccessfully separated clearly SSRs alleles with different sizes of less than 25 bp.

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  • Universitas Gadjah Mada
  • IPB University
  • Muhammadiyah University of Surakarta
  • Cenderawasih University
  • Universitas Bangun Nusantara
  • Institut Teknologi Sepuluh Nopember
  • Universitas Jember
  • Organisasi lain
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Rekan penulisOrganisasiArtikel bersama
Rani Agustina WulandariUniversitas Gadjah Mada3
Kristamtini KristamtiniNational Research and Innovation Agency3
Enik Nurlaili AfifahUniversitas Gadjah Mada2
Sugiharto Budi SantosoMuhammadiyah University of Surakarta2
Paramita CahyaningrumUniversitas Gadjah Mada2
Ratna Dewi EskundariUniversitas Bangun Nusantara2
Didik IndradewaUniversitas Gadjah Mada2
Yekti Asih PurwestriUniversitas Gadjah Mada2
Yunus Pajanjan PaulanganCenderawasih University1
Muh. Arsyad Al. AminIPB University1
Barnabas BarapadangCenderawasih University1
Yudi WahyudinIPB University1
Mahfud MahfudInstitut Teknologi Sepuluh Nopember1
Dimas Tri AsmaraUniversitas Gadjah Mada1
Arman WijonarkoUniversitas Gadjah Mada1
Vega Kartika SariUniversitas Jember1
Vina Eka AristyaUniversitas Gadjah Mada1
Fitri HandayaniAfiliasi tidak diketahui1
Eko HanudinUniversitas Gadjah Mada1
Dewi Ratna NurhayatiUniversitas Slamet Riyadi1
Prapto YudonoUniversitas Gadjah Mada1
Yuli PriyanaMuhammadiyah University of Surakarta1
Suyadi SuyadiUniversity of Brawijaya1
Titin SetyoriniAfiliasi tidak diketahui1
Sapto IndriokoUniversitas Gadjah Mada1
A. RimbawantoAfiliasi tidak diketahui1
Yudi Agus SetiawanIPB University1
Widiatmaka WidiatmakaIPB University1
Wiwin AmbarwulanIPB University1
Hefni EffendiIPB University1
Muhamad Yanuar Jarwadi PurwantoIPB University1
. SUPUTAAfiliasi tidak diketahui1
Gesang PratyadhiraksanaUniversitas Gadjah Mada1
Tri Murniningtyas Puji LestariAfiliasi tidak diketahui1
Hamirul HadiniUniversitas Halu Oleo1
Elisa AnggraeniIPB University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">High Resolution Microsatellite Marker Analysis of Some Rice Landraces Using Metaphor Agarose Gel Electrophoresis</dc:title>
	<dc:creator>Kristamtini, K.</dc:creator>
	<dc:creator>Taryono, T.</dc:creator>
	<dc:creator>Basunanda, Panjisakti</dc:creator>
	<dc:creator>Murti, Rudi Hari</dc:creator>
	<dc:subject xml:lang="en-US">microsatellite markers; Metaphor Agarose Gel Electrophoresis (MAGE); and rice cultivar</dc:subject>
	<dc:description xml:lang="en-US">Microsatellite markers or simple sequences repeats are DNA - based molecular techniques that areused to see the different among accessions and inbred lines. There are three methods to analysis the results ofthe polymerase chain reaction of microsatellite markers namely polyacrylamide gel electrophoresis (PAGE),capillary electroforesis, and Metaphor Agarose Gel Electroforesis (MAGE), and the Use of MAGE assessedmore easily and economically the polymorphic pattern of DNA markers. This study aimed to obtain fast,effective and efficient in term of easy and cheap technique to identify microsatellite markers of some blackrice cultivars and F2 populations from crosses between black with white rice. The results showed that MAGEsuccessfully separated clearly SSRs alleles with different sizes of less than 25 bp .</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-06-08</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/15269</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.15269</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 20, No 1 (2015); 54-61</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/15269/10249</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 K. Kristamtini, Panjisakti Basunanda, Rudi Hari Murti</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>