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Black Rice Bran Extracts and Fractions Containing Cyanidin 3-glucoside and Peonidin 3-glucoside Induce Apoptosis in Human Cervical Cancer Cells

Indonesian Journal of Biotechnology · Vol. 20 No. 1 (2015) · hlm. 69–76

DOI
10.22146/ijbiotech.15271
ISSN
2089-2241, 0853-8654
Terbit
2015-06-08
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Anthocyanin of pigmented rice inhibits the growth of cancer cells. The cytotoxicity and apoptosisinducing properties of local black rice (cv Cempo Ireng) extracts and fractions, which contain anthocyaninincluding cyanidin 3-glucoside and peonidin 3-glucoside, on human cervical cancer cell line (HeLa cells) hasbeen evaluated. The pigmented rice bran was extracted and fractionated using methanol-HCl. The MTT testwas performed on HeLa cell cultures to observe the IC50 value. Preparative TLC was performed to obtain thefractions of black rice bran. Cyanidin 3-glucoside and peonidin 3-glucoside were identified in the pigmentedrice bran extract and fractions using UHPLC. Flowcytometry analysis was performed to measure the percentageof apoptotic cells. Our results suggest that the fractions are more toxic than the methanolic crude extract withIC50 values of 85.95 ± 5.56 μg/mL (the lowest one) and 408.13 ± 51.9 μg/mL, respectively. The concentration ofcyanidin 3-glucoside and peonidin 3-glucoside in the methanolic extract were 1.89 and 0.84 μg/mg, respectively.The apoptosis induction by fractions F2 and F4 (52 and 55%) were significantly higher compared to fractionF3 and F5 (30 and 33%) and doxorubicin (21%). Cyanidin 3-glucoside was detected in F4 (0.14 μg/ml) whilepeonidin 3-glucoside in F2 (0.012 μg/ml), however both were not detected in F3 and F5.

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  • Universitas Gadjah Mada
  • Universitas Trisakti
  • Muhammadiyah University of Yogyakarta
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Rumiyati RumiyatiAfiliasi tidak diketahui5
M. MurwantokoAfiliasi tidak diketahui3
Firman AlamsyahAfiliasi tidak diketahui3
Yekti Asih PurwestriAfiliasi tidak diketahui3
Pudji AstutiUniversitas Trisakti2
Rita MalizaAfiliasi tidak diketahui1
Putra SantosoAfiliasi tidak diketahui1
Bramadi AryaAfiliasi tidak diketahui1
Alimuddin TofrizalAfiliasi tidak diketahui1
Suwarno HadisusantoUniversitas Gadjah Mada1
HaryantiAfiliasi tidak diketahui1
Sari Budi Moria SembiringAfiliasi tidak diketahui1
Jhon Harianto HutapeaAfiliasi tidak diketahui1
I Nyoman Adiasmara GiriAfiliasi tidak diketahui1
Ketut MahardikaAfiliasi tidak diketahui1
Christina Retna HandayaniAfiliasi tidak diketahui1
Asmarani KusumawatiAfiliasi tidak diketahui1
Penny Humaidah HamidAfiliasi tidak diketahui1
Masashi KawaichiAfiliasi tidak diketahui1
Dewi Nur'aeni SetyowatiAfiliasi tidak diketahui1
Rifqi Zahroh JanatunaimAfiliasi tidak diketahui1
Radhiyah Mardhiyah HamidAfiliasi tidak diketahui1
Ghea Putri ChristyAfiliasi tidak diketahui1
Nastiti WijayantiAfiliasi tidak diketahui1
Aushia Tanzih Al HaqAfiliasi tidak diketahui1
T. TrijokoAfiliasi tidak diketahui1
Ch. Retna HandayaniAfiliasi tidak diketahui1
Pratiwi ApridamayantiAfiliasi tidak diketahui1
Woro Anindito Sri TunjungAfiliasi tidak diketahui1
Rizal Maarif RukmanaAfiliasi tidak diketahui1
Nyoman Puniawati SoesiloAfiliasi tidak diketahui1
Sugeng RiyantoMuhammadiyah University of Yogyakarta1
Subagus WahyuonoAfiliasi tidak diketahui1
Yuli WidiyastutiAfiliasi tidak diketahui1
Bambang RetnoajiUniversitas Gadjah Mada1
Endah Sri PalupiAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Black Rice Bran Extracts and Fractions Containing Cyanidin 3-glucoside and Peonidin 3-glucoside Induce Apoptosis in Human Cervical Cancer Cells</dc:title>
	<dc:creator>Pratiwi, Rarastoeti</dc:creator>
	<dc:creator>Tunjung, Woro Anindito Sri</dc:creator>
	<dc:creator>Rumiyati, R.</dc:creator>
	<dc:creator>Amalia, Alfi Rizqi</dc:creator>
	<dc:subject xml:lang="en-US">Cempo Ireng; cyanidine 3-glucoside; peonidin 3-glucoside; apoptosis; HeLa cells</dc:subject>
	<dc:description xml:lang="en-US">Anthocyanin of pigmented rice inhibits the growth of cancer cells. The cytotoxicity and apoptosisinducing properties of local black rice (cv Cempo Ireng) extracts and fractions, which contain anthocyaninincluding cyanidin 3-glucoside and peonidin 3-glucoside, on human cervical cancer cell line (HeLa cells) hasbeen evaluated. The pigmented rice bran was extracted and fractionated using methanol-HCl. The MTT testwas performed on HeLa cell cultures to observe the IC50 value. Preparative TLC was performed to obtain thefractions of black rice bran. Cyanidin 3-glucoside and peonidin 3-glucoside were identified in the pigmentedrice bran extract and fractions using UHPLC. Flowcytometry analysis was performed to measure the percentageof apoptotic cells. Our results suggest that the fractions are more toxic than the methanolic crude extract withIC50 values of 85.95 ± 5.56 μg/mL (the lowest one) and 408.13 ± 51.9 μg/mL, respectively. The concentration ofcyanidin 3-glucoside and peonidin 3-glucoside in the methanolic extract were 1.89 and 0.84 μg/mg, respectively.The apoptosis induction by fractions F2 and F4 (52 and 55%) were significantly higher compared to fractionF3 and F5 (30 and 33%) and doxorubicin (21%). Cyanidin 3-glucoside was detected in F4 (0.14 μg/ml) whilepeonidin 3-glucoside in F2 (0.012 μg/ml), however both were not detected in F3 and F5.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-06-08</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/15271</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.15271</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 20, No 1 (2015); 69-76</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/15271/10250</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 Rarastoeti Pratiwi, Woro Anindito Sri Tunjung, R. Rumiyati, Alfi Rizqi Amalia</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>