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Induction of Somatic Embryogenesis through Overexpression of ATRKD4 Genes in Phalaenopsis “Sogo Vivien”

Indonesian Journal of Biotechnology · Vol. 20 No. 1 (2015) · hlm. 42–53

DOI
10.22146/ijbiotech.15276
ISSN
2089-2241, 0853-8654
Terbit
2015-06-08
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Phalaenopsis “Sogo Vivien “is a mini orchid hybrid with beautiful flowers and numerous inflorescences.Mass propagation of this orchid is needed to meet the market demand. Objective of this research was toinduce somatic embryogenesis of P.”Sogo Vivien” through insertion of AtRKD4 gene into orchid. T-DNAcontaining 35S::GAL4::AtRKD4::GR was inserted into 16-22 days after sowing orchid protocorms mediated byAgrobacterium tumefaciens EHA 105. Activation of the AtRKD4 gene was induced by glucocorticoid inductionsystem, using 15μM Dexamethasone (Dex). The results showed that 34 out of 2,648 orchid embryos developedinto protocorms on hygromycin selection medium, whereas only 4 out of 2,897 non-transformant protocormsdeveloped from embryos. A 500 bp of HPT genes was amplified from transformant candidates using specificprimers for HPT (HygF1 and HygR1) and 380 bp was amplified using specific primers for AtRKD4 (AtRKD4F1 and AtRKD4 R1), indicated that transgenes have been integrated into orchid genomes. Finally, 17 plantletswere positively carrying AtRKD4 and HPT genes, the efficiency of transformation was 0.63 %. Somatic embryoswere also emerged from leaf explants of transformant on hormone-free NP medium and became normalplantlets. It is probably due to the high activity of AtRKD4 genes in orchids

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  • Universitas Gadjah Mada
  • Indonesian Coffee and Cocoa Research Institute
  • National Research and Innovation Agency
  • Universitas Pattimura
  • University of Warwick
  • Diponegoro University
  • University of Malaya
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Rekan penulisOrganisasiArtikel bersama
Ari IndriantoUniversitas Gadjah Mada7
Muhammad Dylan LawrieUniversitas Gadjah Mada7
Nintya SetiariDiponegoro University6
Agus Budi SetiawanUniversitas Gadjah Mada5
Windi MoseUniversitas Pattimura4
Febri Yuda KurniawanUniversitas Gadjah Mada3
Jose Gutierrez‐MarcosUniversity of Warwick3
Jaka WidadaUniversitas Gadjah Mada3
Budi Setiadi DaryonoUniversitas Gadjah Mada3
Aries Bagus SasongkoUniversitas Gadjah Mada3
Dyah Weny RespatieUniversitas Gadjah Mada2
Chee How TeoUniversity of Malaya2
Aviesta LinggabuwanaUniversitas Gadjah Mada2
Saifa Usni PutriUniversitas Gadjah Mada2
Nuzlan RasjidUniversitas Gadjah Mada2
Fitriana PuspitasariUniversitas Gadjah Mada2
Naufal Ghozi Aditya PerdanaUniversitas Gadjah Mada2
Dika SundariUniversitas Gadjah Mada2
Triono Bagus SaputroInstitut Teknologi Sepuluh Nopember2
Zulfa LayinaUniversitas Gadjah Mada2
Della Rosiana NingtiasUniversitas Gadjah Mada2
Falah Nur AlifiantoUniversitas Gadjah Mada2
Asadi AsadiAfiliasi tidak diketahui1
Sahiral YakubAfiliasi tidak diketahui1
Ari WibowoIndonesian Coffee and Cocoa Research Institute1
Tri Joko RaharjoState University of Semarang1
Stalis Norma EthicaUniversitas Muhammadiyah Semarang1
Oedjijono OedjijonoJenderal Soedirman University1
Irna AuliauzzakiaUniversitas Gadjah Mada1
Binti Nur AisahUniversitas Gadjah Mada1
Aprilia Tiara IkhwaniUniversitas Gadjah Mada1
Erlina AmbarwatiUniversitas Gadjah Mada1
Listy AnggraeniNational Research and Innovation Agency1
Chandra SetyawanUniversitas Gadjah Mada1
Aniswatul KhamidahNational Research and Innovation Agency1
Rina ArimarsetiowatiIndonesian Coffee and Cocoa Research Institute1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Induction of Somatic Embryogenesis through Overexpression of ATRKD4 Genes in Phalaenopsis “Sogo Vivien”</dc:title>
	<dc:creator>Mursyanti, Exsyupransia</dc:creator>
	<dc:creator>Purwantoro, Aziz</dc:creator>
	<dc:creator>Moeljopawiro, Sukarti</dc:creator>
	<dc:creator>Semiarti, Endang</dc:creator>
	<dc:subject xml:lang="en-US">AtRKD4; glucocorticoid; Phalaenopsis “Sogo Vivien”; orchid hybrid; somatic embryo</dc:subject>
	<dc:description xml:lang="en-US">Phalaenopsis “Sogo Vivien “is a mini orchid hybrid with beautiful flowers and numerous inflorescences.Mass propagation of this orchid is needed to meet the market demand. Objective of this research was toinduce somatic embryogenesis of P.”Sogo Vivien” through insertion of AtRKD4 gene into orchid. T-DNAcontaining 35S::GAL4::AtRKD4::GR was inserted into 16-22 days after sowing orchid protocorms mediated byAgrobacterium tumefaciens EHA 105. Activation of the AtRKD4 gene was induced by glucocorticoid inductionsystem, using 15μM Dexamethasone (Dex). The results showed that 34 out of 2,648 orchid embryos developedinto protocorms on hygromycin selection medium, whereas only 4 out of 2,897 non-transformant protocormsdeveloped from embryos. A 500 bp of HPT genes was amplified from transformant candidates using specificprimers for HPT (HygF1 and HygR1) and 380 bp was amplified using specific primers for AtRKD4 (AtRKD4F1 and AtRKD4 R1), indicated that transgenes have been integrated into orchid genomes. Finally, 17 plantletswere positively carrying AtRKD4 and HPT genes, the efficiency of transformation was 0.63 %. Somatic embryoswere also emerged from leaf explants of transformant on hormone-free NP medium and became normalplantlets. It is probably due to the high activity of AtRKD4 genes in orchids</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-06-08</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/15276</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.15276</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 20, No 1 (2015); 42-53</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/15276/10254</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 Exsyupransia Mursyanti, Aziz Purwantoro, Sukarti Moeljopawiro, Endang Semiarti</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>