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Effect of Oxidative Stress on AhpC Activity and Virulence in katG Ser315 Thr Mycobacterium tuberculosis Mutant

Indonesian Journal of Biotechnology · Vol. 16 No. 2 (2011) · hlm. 100–110

DOI
10.22146/ijbiotech.16369
ISSN
2089-2241, 0853-8654
Terbit
2011-12-06
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Mycobacterium tuberculosis strains resistance to INH is mainly caused by the alteration in several genesencoding the molecular targets. Mutation of katG at codon 315 especially Ser315Thr are responsible forINH resistance in a large proportion of TB cases. The aim of this study is to evaluate the influence of stressoxidative on AhpC activity of katG Ser315Thr of M.tuberculosis, and to find out the relation of AhpC and thevirulence of this mutant. The study design was laboratoric experimental, subjects of study were M.tuberculosisINH resistance strains, and the treatment were serial dose of H2O2. Eighty five M.tuberculosis INH resistantclinical strain were screened for mutation of katGSer315Thr by PCR/RFLP and characterized on the basis ofphenotypic properties (catalase activity and AhpC activity). AhpC activity of katG Ser315Thr M.tuberculosisstrains in response to oxidative stress condition was evaluated by culturing the strains on liquid culturemedium containing 1mM H2O2.To ascertain role of AhpC in the virulence of katGSer315Thr mutant strains, themutants were infected into human macrophages culture, and several indicator of virulence were observed (i.e:replication competence, and apoptosis induction on human macrophages). The results showed that katG Ser315Thr were identified in 23 (27,05%) of 85 INH resistance strains, all mutant strains had decrease of catalaseactivity. AhpC activity of katG Ser315Thr of M.tuberculosis increased significantly with increase of hydrogenperoxide dose. In addition, it has been shown that increased AhpC activity related to replication ability ofmutant, and reduction of apoptosis macrophages induction significantly. We conclude that the productionof AhpC of katG Ser315Thr M.tuberculosis induced by oxidative stress. There was a role of AhpC in virulenceof the M.tuberculosis katG Ser315Thr strains by replication capability and macrophages apoptosis.

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  • Universitas Gadjah Mada
  • Sriwijaya University
  • Universitas Negeri Surabaya
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Rekan penulisOrganisasiArtikel bersama
Michael Haryadi WibowoAfiliasi tidak diketahui4
Jaka WidadaUniversitas Gadjah Mada4
Nastiti WijayantiAfiliasi tidak diketahui3
Wayan T. ArtamaAfiliasi tidak diketahui3
Arum SetiawanSriwijaya University2
Mammed SagiUniversitas Gadjah Mada2
Istriyati IstriyatiUniversitas Gadjah Mada2
Marsetyawan HNE SoesatyoAfiliasi tidak diketahui2
Hera NirwatiAfiliasi tidak diketahui2
Farida J. RachmawatyAfiliasi tidak diketahui2
Marsetyawan H.N.E. SoesatyoAfiliasi tidak diketahui2
M. MustofaAfiliasi tidak diketahui2
A. AlimuddinAfiliasi tidak diketahui2
SEBASTIAN MARGINOAfiliasi tidak diketahui2
W. WidodoUniversitas Negeri Surabaya2
Sri DarmawatiAfiliasi tidak diketahui2
LANGKAH SEMBIRINGAfiliasi tidak diketahui2
Tri UntariAfiliasi tidak diketahui2
Diani MentariAfiliasi tidak diketahui1
Nastiti WijayantiUniversitas Gadjah Mada1
Pinandi Sri PudyaniAfiliasi tidak diketahui1
Ika Dewi AnaAfiliasi tidak diketahui1
Tita Ratya UtariAfiliasi tidak diketahui1
Siti SunarintyasAfiliasi tidak diketahui1
Juni HandajaniAfiliasi tidak diketahui1
Yulita KristantiAfiliasi tidak diketahui1
Risya CilmiatyAfiliasi tidak diketahui1
Mandojo RukmoAfiliasi tidak diketahui1
Afiono Agung PrasetyoAfiliasi tidak diketahui1
Khilyat Ulin Nur ZainiAfiliasi tidak diketahui1
Suhartono Taat PutraAfiliasi tidak diketahui1
Banun KusumawardaniAfiliasi tidak diketahui1
Djaswadi DasukiAfiliasi tidak diketahui1
Al. Supartinah SantosoAfiliasi tidak diketahui1
Regina TC. TandelilinAfiliasi tidak diketahui1
Abdul Salam M. SofroAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Effect of Oxidative Stress on AhpC Activity and Virulence in katG Ser315 Thr Mycobacterium tuberculosis Mutant</dc:title>
	<dc:creator>Rintiswati, Ning</dc:creator>
	<dc:creator>Wibawa, Tri</dc:creator>
	<dc:creator>Asmara, Widya</dc:creator>
	<dc:creator>Soebono, Hardyanto</dc:creator>
	<dc:subject xml:lang="en-US">katG Ser315Thr Mycobacterium tuberculosis; oxidative stress; AhpC; virulence</dc:subject>
	<dc:description xml:lang="en-US">Mycobacterium tuberculosis strains resistance to INH is mainly caused by the alteration in several genesencoding the molecular targets. Mutation of katG at codon 315 especially Ser315Thr are responsible forINH resistance in a large proportion of TB cases. The aim of this study is to evaluate the influence of stressoxidative on AhpC activity of katG Ser315Thr of M.tuberculosis, and to find out the relation of AhpC and thevirulence of this mutant. The study design was laboratoric experimental, subjects of study were M.tuberculosisINH resistance strains, and the treatment were serial dose of H2O2. Eighty five M.tuberculosis INH resistantclinical strain were screened for mutation of katGSer315Thr by PCR/RFLP and characterized on the basis ofphenotypic properties (catalase activity and AhpC activity). AhpC activity of katG Ser315Thr M.tuberculosisstrains in response to oxidative stress condition was evaluated by culturing the strains on liquid culturemedium containing 1mM H2O2.To ascertain role of AhpC in the virulence of katGSer315Thr mutant strains, themutants were infected into human macrophages culture, and several indicator of virulence were observed (i.e:replication competence, and apoptosis induction on human macrophages). The results showed that katG Ser315Thr were identified in 23 (27,05%) of 85 INH resistance strains, all mutant strains had decrease of catalaseactivity. AhpC activity of katG Ser315Thr of M.tuberculosis increased significantly with increase of hydrogenperoxide dose. In addition , it has been shown that increased AhpC activity related to replication ability ofmutant, and reduction of apoptosis macrophages induction significantly. We conclude that the productionof AhpC of katG Ser315Thr M.tuberculosis induced by oxidative stress. There was a role of AhpC in virulenceof the M.tuberculosis katG Ser315Thr strains by replication capability and macrophages apoptosis.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2011-12-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/16369</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.16369</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 16, No 2 (2011); 100-110</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/16369/10897</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 Ning Rintiswati, Tri Wibawa, Widya Asmara, Hardyanto Soebono</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>