Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Synergistic effects of para-hydroxy meta-methoxy chalcone (pHmMC)- doxorubicin treatments on T47D breast cancer cells

Indonesian Journal of Biotechnology · Vol. 20 No. 2 (2015) · hlm. 141–151

DOI
10.22146/ijbiotech.24197
ISSN
2089-2241, 0853-8654
Terbit
2015-12-01
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Resistance to some cancer chemotherapeutic drugs has been identifed. One strategy to overcome that problem is by combining two or more of the drugs to get co-chemotherapeutic effects. A derivate chalcone, 3 - (4’-hydroxy-3’-methoxyphenyl)-1-phenyl-2-propene-1-on or para hydroxy meta methoxy chalcone (pHmMC), has been reported to have cytotoxic activity on some cancer cells through some pathways. The aim of this study was to investigate the effects of combinations of pHmMC and Doxorubicin (Dox) on the cytotoxicity, anti-proliferation, apoptosis, and the cell cycle of T47D (breast cancer cell-lines) in vitro. The cytotoxic and antiproliferative activity were determined by MTT (3-[4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide] assay. The combination index (CI) was used to determine the synergistic, additive or antagonistic effects of the combinations. Flowcytometry method was performed to determine the combination effects on the apoptosis and cell cycle. The results indicated that the combinations had a higher inhibitory effect on the cell growth compared to those of single treatments of pHmMC and Dox. All the doses used in the combinations were lower of the single doses at their IC50s. The results showed all the combinations gave synergistic (CI: 0.3 – 0.7) up to strong synergistic (CI: 0.1 – 0.3) effects. The synergistic effects of the combinations were due to increased apoptosis and induced cell cycle arrest in S and G2/M phases on the cancer cell lines.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 122 tautan kepenulisan bersama · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • University of Brawijaya
  • IPB University
  • Universitas Gadjah Mada
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Ratna Asmah SusidartiAfiliasi tidak diketahui17
Adam HermawanAfiliasi tidak diketahui17
Riris Istighfari JenieAfiliasi tidak diketahui14
Muthi’ IkawatiAfiliasi tidak diketahui11
Rohmad Yudi UtomoAfiliasi tidak diketahui8
Dyaningtyas Dewi Pamungkas PutriAfiliasi tidak diketahui7
Sari HaryantiAfiliasi tidak diketahui5
Dhania NovitasariAfiliasi tidak diketahui4
Retno MurwantiAfiliasi tidak diketahui4
Faaza Aulia RahmanAfiliasi tidak diketahui4
Desty Restia RahmawatiAfiliasi tidak diketahui4
Sri HandayaniUniversity of Brawijaya3
Ummi Maryam ZulfinAfiliasi tidak diketahui3
Anindyajati AnindyajatiAfiliasi tidak diketahui3
Larasati LarasatiIPB University3
Erlina RivantiAfiliasi tidak diketahui3
Umar Anggara JenieAfiliasi tidak diketahui3
Sri AtunAfiliasi tidak diketahui2
Masashi KawaichiAfiliasi tidak diketahui2
Sendy JunediAfiliasi tidak diketahui2
Nunuk Aries NurulitaAfiliasi tidak diketahui2
Suwijiyo PramonoAfiliasi tidak diketahui2
Zalinar UdinAfiliasi tidak diketahui2
Yogi ErtantoAfiliasi tidak diketahui2
Lailatul QodriaAfiliasi tidak diketahui2
Gergorius Gena MaranAfiliasi tidak diketahui2
Aulia Nur SeptianiAfiliasi tidak diketahui2
Sofa FaridaAfiliasi tidak diketahui2
Beni LestariAfiliasi tidak diketahui2
Asri Mega PutriAfiliasi tidak diketahui2
Hilyatul FadliyahAfiliasi tidak diketahui2
Indri KusharyantiAfiliasi tidak diketahui2
Yonika Arum LarasatiAfiliasi tidak diketahui2
Raditya Prima IstiajiAfiliasi tidak diketahui2
Ni Putu Linda LaksmianiAfiliasi tidak diketahui2
Jaka WidadaUniversitas Gadjah Mada2

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Synergistic effects of para-hydroxy meta-methoxy chalcone (pHmMC)- doxorubicin treatments on T47D breast cancer cells</dc:title>
	<dc:creator>Arianingrum, Retno</dc:creator>
	<dc:creator>Sunarminingsih, Retno</dc:creator>
	<dc:creator>Meiyanto, Edy</dc:creator>
	<dc:creator>Mubarika, Sofia</dc:creator>
	<dc:subject xml:lang="en-US">pHmMC; Dox; co-chemotherapy; T47D cells</dc:subject>
	<dc:description xml:lang="en-US">Resistance to some cancer chemotherapeutic drugs has been identifed. One strategy to overcome that problem is by combining two or more of the drugs to get co-chemotherapeutic effects. A derivate chalcone, 3 - (4’-hydroxy-3’-methoxyphenyl)-1-phenyl-2-propene-1-on or para hydroxy meta methoxy chalcone (pHmMC), has been reported to have cytotoxic activity on some cancer cells through some pathways. The aim of this study was to investigate the effects of combinations of pHmMC and Doxorubicin (Dox) on the cytotoxicity, anti-proliferation, apoptosis, and the cell cycle of T47D (breast cancer cell-lines) in vitro. The cytotoxic and antiproliferative activity were determined by MTT (3-[4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide] assay. The combination index (CI) was used to determine the synergistic, additive or antagonistic effects of the combinations. Flowcytometry method was performed to determine the combination effects on the apoptosis and cell cycle. The results indicated that the combinations had a higher inhibitory effect on the cell growth compared to those of single treatments of pHmMC and Dox. All the doses used in the combinations were lower of the single doses at their IC50s. The results showed all the combinations gave synergistic (CI: 0.3 – 0.7) up to strong synergistic (CI: 0.1 – 0.3) effects. The synergistic effects of the combinations were due to increased apoptosis and induced cell cycle arrest in S and G2/M phases on the cancer cell lines.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-12-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/24197</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.24197</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 20, No 2 (2015); 141-151</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/24197/15813</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>