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Identifcation of antibiotic producing endophytic microbe isolates from a national park in Java island

Indonesian Journal of Biotechnology · Vol. 20 No. 2 (2015) · hlm. 167–173

DOI
10.22146/ijbiotech.24345
ISSN
2089-2241, 0853-8654
Terbit
2015-12-01
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Endophytic microbes are potential sources of antibiotics. Some numbers of endophytic bacteria were isolated from plants in Ujung Kulon, Kaliurang, Meru Betiri and Baluran National Park, Bogor Botanical Garden, and Nusakambangan forest, Indonesia. Previous studies have been conducted to examine and obtain endophytic bacteria isolates from the selected plants, which resulted in three selected isolates, namely OOH-1, STG-1, and CMB-2. This research was conducted to determine the molecular identity of OOH-1 and STG-1 isolates, as well as to identify antibiotic compounds produced by STG-1 isolate. Molecular identifcation of selected isolates was based on 16S rRNA gene analysis and amplifed using primers 27F and 1492R. A phylogeny tree was then constructed by comparing the resulting sequences with data from Gene Bank using the BLAST-N program. The identifcation showed that STG-1 isolate had a 99% similarity with Pseudomonas brenneri strain SFML 97-391, and OOH-1 isolate had a 99% similarity with Enterobacter xiangfangensis. Identifcation of antibiotic compounds was done by purifcation and separation of the compounds. Antibiotic activity was also examined based on Lethal Concentration (LC50) on Fusarium oxysporum with a LC50 of 0.01–0.02% against Fusarium oxysporum.

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  • Universitas Gadjah Mada
  • Universitas Halu Oleo
  • IPB University
  • State University of Makassar
  • National Research and Innovation Agency
  • Diponegoro University
  • Universitas Bhamada Slawi
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Rekan penulisOrganisasiArtikel bersama
Puji AstutiUniversitas Gadjah Mada2
Nur Arfa YantiUniversitas Halu Oleo2
Langkah SembiringUniversitas Gadjah Mada2
Erni MartaniUniversitas Gadjah Mada2
Widya AsmaraUniversitas Gadjah Mada2
Tri Rini NuringtyasUniversitas Gadjah Mada2
Muhammad Saifur RohmanUniversitas Gadjah Mada2
Ririn SuharsantiAfiliasi tidak diketahui1
Nunung YuniartiUniversitas Gadjah Mada1
S. M. WidyastutiUniversitas Gadjah Mada1
Etik Erna Wati HadiAfiliasi tidak diketahui1
Sri BudiartiIPB University1
Siti Muslimah WidyastutiUniversitas Gadjah Mada1
Medhina MagdalenaAfiliasi tidak diketahui1
Agustinus Joko NugrohoAfiliasi tidak diketahui1
Chatarina BeharAfiliasi tidak diketahui1
Nurhayani Haji MuhiddinState University of Makassar1
N. NgadimanUniversitas Gadjah Mada1
J. Jumi'atiAfiliasi tidak diketahui1
Andriessa PrameswaraUniversitas Gadjah Mada1
Sukarti MoeljopawiroUniversitas Gadjah Mada1
Yoga Mulia PratamaUniversitas Gadjah Mada1
G. GalihUniversitas Gadjah Mada1
Yuli WidiyastutiNational Research and Innovation Agency1
Rarastoeti PratiwiUniversitas Gadjah Mada1
Sugeng RiyantoUniversitas Gadjah Mada1
Listia PradaniUniversitas Gadjah Mada1
Donny WidiantoUniversitas Gadjah Mada1
Adinda Fitri SalsabilaUniversitas Gadjah Mada1
Abidah TauchidUniversitas Gadjah Mada1
Nastiti WijayantiUniversitas Gadjah Mada1
Dian Ayuning TyasUniversitas Gadjah Mada1
2, Wilis Ari Ari Setyati1Afiliasi tidak diketahui1
Triyanto TriyantoUniversitas Gadjah Mada1
Subagiyo SubagiyoDiponegoro University1
Oktariani PramiastutiUniversitas Bhamada Slawi1
Nanang FakhrudinUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Identifcation of antibiotic producing endophytic microbe isolates from a national park in Java island</dc:title>
	<dc:creator>Yuwantiningsih, Sri</dc:creator>
	<dc:creator>Margino, Sebastian</dc:creator>
	<dc:creator>Wahyuono, Subagus</dc:creator>
	<dc:subject xml:lang="en-US">antibiotic; endophytic microbes; molecular identifcation</dc:subject>
	<dc:description xml:lang="en-US">Endophytic microbes are potential sources of antibiotics. Some numbers of endophytic bacteria were isolated from plants in Ujung Kulon, Kaliurang, Meru Betiri and Baluran National Park, Bogor Botanical Garden, and Nusakambangan forest, Indonesia. Previous studies have been conducted to examine and obtain endophytic bacteria isolates from the selected plants, which resulted in three selected isolates, namely OOH-1, STG-1, and CMB-2. This research was conducted to determine the molecular identity of OOH-1 and STG-1 isolates, as well as to identify antibiotic compounds produced by STG-1 isolate. Molecular identifcation of selected isolates was based on 16S rRNA gene analysis and amplifed using primers 27F and 1492R. A phylogeny tree was then constructed by comparing the resulting sequences with data from Gene Bank using the BLAST-N program. The identifcation showed that STG-1 isolate had a 99% similarity with Pseudomonas brenneri strain SFML 97-391, and OOH-1 isolate had a 99% similarity with Enterobacter xiangfangensis. Identifcation of antibiotic compounds was done by purifcation and separation of the compounds. Antibiotic activity was also examined based on Lethal Concentration (LC50) on Fusarium oxysporum with a LC50 of 0.01–0.02% against Fusarium oxysporum.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-12-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/24345</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.24345</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 20, No 2 (2015); 167-173</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/24345/15838</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>