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Brazilein in combination with cisplatin inhibit proliferation and migration on highly metastatic cancer cells, 4T1

Indonesian Journal of Biotechnology · Vol. 21 No. 1 (2016) · hlm. 38–47

DOI
10.22146/ijbiotech.26106
ISSN
2089-2241, 0853-8654
Terbit
2016-06-01
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Brazilein performs anti­cancer activities on several cancer cells and potentially inhibits metastasis. The aims of this study is to observe the synergistic cytotoxic and migration inhibitory effect of brazilein combined with cisplatin on 4T1 breast cancer cells. Under MTT assay, we found that brazilein revealed cytotoxic effect on 4T1 cells in a dose­dependent manner (IC50=50 ± 0.3 µM). Combination of brazilein and cisplatin showed synergistic effect (CI=0.72). Flowcytometry analysis on the cell cycle progression showed that single treatment of 25 µM brazilein induced G2/M­phase accumulation, 12.5 µM cisplatin induced S­phase accumulation, while combination of brazilein and cisplatin induced S­phase and G2/Mphase accumulation. Combination of brazilein and cisplatin induced apoptosis higher than that of the single treatments. Based on wound healing assay, 12.5 µM brazilein and its combination with 6.25 µM cisplatin inhibited cells migration. Immunoblotting and gelatin zymography analysis showed that combination of brazilein and cisplatin inhibited the expression level of Rac1 and MMP9 proteins. Based on these results, we conclude that brazilein enhanced cytotoxic activity of cisplatin and inhibited migration on 4T1 cells and potentially can be developed as an enhancing cytotoxic and antimetastasis agent.

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  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Kementerian Kesehatan Republik Indonesia
  • Nara Institute of Science and Technology
  • Atma Jaya University Yogyakarta
  • Muhammadiyah University Purwokerto
  • Sunan Kalijaga State Islamic University Yogyakarta
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Rekan penulisOrganisasiArtikel bersama
Adam HermawanUniversitas Gadjah Mada23
Muthi IkawatiUniversitas Gadjah Mada15
Rohmad Yudi UtomoUniversitas Gadjah Mada14
Dyaningtyas Dewi Pamungkas PutriUniversitas Gadjah Mada7
Faaza Aulia RahmanUniversitas Gadjah Mada7
Dhania NovitasariUniversitas Gadjah Mada7
Desty Restia RahmawatiUniversitas Gadjah Mada6
Sari HaryantiKementerian Kesehatan Republik Indonesia5
Larasati LarasatiUniversitas Gadjah Mada5
Yogi ErtantoUniversitas Gadjah Mada5
Retno MurwantiUniversitas Gadjah Mada4
Ummi Maryam ZulfinUniversitas Gadjah Mada4
Annisa NovarinaUniversitas Gadjah Mada4
Hilyatul FadliyahUniversitas Gadjah Mada4
Indri KusharyantiTanjungpura University4
Anindyajati AnindyajatiUniversitas Gadjah Mada3
Erlina RivantiUniversitas Gadjah Mada3
Sendy JunediAtma Jaya University Yogyakarta3
Gergorius Gena MaranUniversitas Gadjah Mada3
Aulia Nur SeptianiUniversitas Gadjah Mada3
Sofa FaridaUniversitas Gadjah Mada3
Beni LestariUniversitas Gadjah Mada3
Laeli MuntafiahUniversitas Gadjah Mada3
Rumiyati RumiyatiUniversitas Gadjah Mada3
Yurista GilangUniversitas Gadjah Mada3
Raisatun Nisa SugiyantoUniversitas Gadjah Mada3
Masashi KawaichiNara Institute of Science and Technology2
Nunuk Aries NurulitaMuhammadiyah University Purwokerto2
Suwijiyo PramonoUniversitas Gadjah Mada2
Lailatul QodriaUniversitas Gadjah Mada2
Laili Nailul MunaSunan Kalijaga State Islamic University Yogyakarta2
Asri Mega PutriUniversitas Gadjah Mada2
Bani Adlina ShabrinaUniversitas Gadjah Mada2
Nur Fitra SariUniversitas Gadjah Mada2
Fatiha Citra EffendiUniversitas Gadjah Mada2

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Brazilein in combination with cisplatin inhibit proliferation and migration on highly metastatic cancer cells, 4T1</dc:title>
	<dc:creator>Handayani, Sri</dc:creator>
	<dc:creator>Susidarti, Ratna Asmah</dc:creator>
	<dc:creator>Udin, Zalinar</dc:creator>
	<dc:creator>Meiyanto, Edy</dc:creator>
	<dc:creator>Jenie, Riris Istighfari</dc:creator>
	<dc:subject xml:lang="en-US">brazilein; cisplatin; combination treatment; cytotoxic effect; cells migration</dc:subject>
	<dc:description xml:lang="en-US">Brazilein performs anti­cancer activities on several cancer cells and potentially inhibits metastasis. The aims of this study is to observe the synergistic cytotoxic and migration inhibitory effect of brazilein combined with cisplatin on 4T1 breast cancer cells. Under MTT assay, we found that brazilein revealed cytotoxic effect on 4T1 cells in a dose­dependent manner (IC50=50 ± 0.3 µM). Combination of brazilein and cisplatin showed synergistic effect (CI=0.72). Flowcytometry analysis on the cell cycle progression showed that single treatment of 25 µM brazilein induced G2/M­phase accumulation, 12.5 µM cisplatin induced S­phase accumulation, while combination of brazilein and cisplatin induced S­phase and G2/Mphase accumulation. Combination of brazilein and cisplatin induced apoptosis higher than that of the single treatments. Based on wound healing assay, 12.5 µM brazilein and its combination with 6.25 µM cisplatin inhibited cells migration. Immunoblotting and gelatin zymography analysis showed that combination of brazilein and cisplatin inhibited the expression level of Rac1 and MMP9 proteins. Based on these results, we conclude that brazilein enhanced cytotoxic activity of cisplatin and inhibited migration on 4T1 cells and potentially can be developed as an enhancing cytotoxic and antimetastasis agent.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2016-06-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/26106</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.26106</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 21, No 1 (2016); 38-47</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/26106/16534</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>