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Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia

Indonesian Journal of Biotechnology · Vol. 22 No. 1 (2017) · hlm. 49–54

DOI
10.22146/ijbiotech.26401
ISSN
2089-2241, 0853-8654
Terbit
2017-06-01
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Andalas University
  • Tanjungpura University
  • Universitas Al-Azhar Indonesia
  • Nara Institute of Science and Technology
  • Universitas Muhammadiyah Pontianak
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Rekan penulisOrganisasiArtikel bersama
Ardaning NurilianiUniversitas Gadjah Mada5
Alfi Rizqi AmaliaUniversitas Gadjah Mada4
M. MurwantokoAfiliasi tidak diketahui3
Langkah SembiringUniversitas Gadjah Mada3
Didik IndradewaUniversitas Gadjah Mada3
Firman AlamsyahUniversitas Al-Azhar Indonesia3
Radhiyah Mardhiyah HamidUniversitas Gadjah Mada3
Rifqi Zahroh JanatunaimUniversitas Gadjah Mada3
Endang SemiartiUniversitas Gadjah Mada3
PUDJI ASTUTIAfiliasi tidak diketahui2
Charlie Ester de FretesNational Research and Innovation Agency2
Tri Rini NuringtyasUniversitas Gadjah Mada2
Donny WidiantoUniversitas Gadjah Mada2
Alfino SebastianUniversitas Gadjah Mada2
Wahyu Aristyaning PutriUniversitas Gadjah Mada2
Hanum Mukti RahayuUniversitas Muhammadiyah Pontianak2
Anis Uswatun KhasanahUniversitas Islam Negeri Sultan Maulana Hasanuddin Banten2
Yuni SineUniversitas Gadjah Mada2
Riris Istighfari JenieUniversitas Gadjah Mada2
Taryono TaryonoUniversitas Gadjah Mada2
Ratna Dewi EskundariUniversitas Bangun Nusantara2
Masashi KawaichiNara Institute of Science and Technology2
Aries Bagus SasongkoUniversitas Gadjah Mada2
Rita MalizaAndalas University1
Putra SantosoAndalas University1
Bramadi AryaMahidol University1
Alimuddin TofrizalAndalas University1
Suwarno HadisusantoUniversitas Gadjah Mada1
Sari Budi Moria SembiringNational Research and Innovation Agency1
Jhon Harianto HutapeaNational Research and Innovation Agency1
I Nyoman Adiasmara GiriNational Research and Innovation Agency1
Ketut MahardikaNational Research and Innovation Agency1
HaryantiNational Research and Innovation Agency1
Bima MahendraUniversitas Gadjah Mada1
Nabila Ramiza PuteriUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia</dc:title>
	<dc:creator>Apridamayanti, Pratiwi</dc:creator>
	<dc:creator>Pratiwi, Rarastoeti</dc:creator>
	<dc:creator>Purwestri, Yekti Asih</dc:creator>
	<dc:creator>Sri Tunjung, Woro Anindito</dc:creator>
	<dc:creator>Rumiyati, Rumiyati</dc:creator>
	<dc:subject xml:lang="en-US">nthocyanin; antioxidant; black rice bran; nutrient contents</dc:subject>
	<dc:description xml:lang="en-US">The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2017-06-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/26401</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.26401</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 22, No 1 (2017); 49-54</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/26401/19527</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>