Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Early detection of the orchid flowering gene PaFT1 in tobacco cells using a GFP reporter

Indonesian Journal of Biotechnology · Vol. 21 No. 1 (2016) · hlm. 12–21

DOI
10.22146/ijbiotech.26781
ISSN
2089-2241, 0853-8654
Terbit
2016-06-01
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Here we describe a novel method of using green fluorescence protein (GFP) as a reporter gene for early detection of an integrated T­DNA containing the orchid flowering gene, PaFT1 (Phalaenopsis aphrodite Flowering locus T1) in the tobacco genome. Functional assays that report the presence of exogenous DNA early in development are especially useful in plants where the desired phenotype is only apparent after long periods of vegetative growth. The objective of this study is to establish a method for detecting an inserted Phalaenopsis orchid flowering gene and examining its function in tobacco. The p35S::PaFT1­ 35S::GFP construct was introduced into Agrobacterium tumefaciens strain EHA101. Transformed tobacco leaves were cultured on MS medium with addition of 1 mgL-1 NAA+3 mgL-1 BAP+50 mgL-1 Kanamycin+300 mgL-1 timentin for selection. Results showed bright green GFP fluorescent signals in 11 out of 15 (73%) tobacco leaf cells at a 2­month time point after transformation. GFP and PaFT1 fragments were amplified in genomic PCR using GFP and PaFT1 specific primers. The accumulated PaFT1 transcripts were observed in 3 month­old transgenic tobacco plants containing p35S::PaFT1­35S::GFP. Green florescence was observed only in the transgenic plants at the 5 month­old stage but not in the wild type controls.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 105 tautan kepenulisan bersama · 36 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Lampung University
  • Academia Sinica
  • Universitas Pattimura
  • University of Warwick
  • Diponegoro University
  • Organisasi lain
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Aziz PurwantoroUniversitas Gadjah Mada18
Ari IndriantoUniversitas Gadjah Mada5
Rina Sri KasiamdariUniversitas Gadjah Mada4
Windi MoseUniversitas Pattimura4
Jose Gutierrez‐MarcosUniversity of Warwick4
Nintya SetiariDiponegoro University4
Exsyupransia MursyantiUniversitas Gadjah Mada4
Arif MuazamUniversitas Gadjah Mada3
Setyorini WidyayantiNational Research and Innovation Agency3
Febri Yuda KurniawanUniversitas Gadjah Mada3
Eko Agus SuyonoUniversitas Gadjah Mada3
Jaka WidadaUniversitas Gadjah Mada3
Aries Bagus SasongkoUniversitas Gadjah Mada3
Naufal Ghozi Aditya PerdanaUniversitas Gadjah Mada3
Purnomo PurnomoUniversitas Gadjah Mada2
Adib Fakhruddin YusufUniversitas Gadjah Mada2
Purnomo PurnomoUniversitas Gadjah Mada2
Ganies Riza AristyaUniversitas Gadjah Mada2
Tuty ArisuryantiUniversitas Gadjah Mada2
Yudhistira NugrahaNational Research and Innovation Agency2
Kristamtini KristamtiniNational Research and Innovation Agency2
Keiko T. NatsuakiTokyo University of Agriculture2
Aviesta LinggabuwanaUniversitas Gadjah Mada2
Saifa Usni PutriUniversitas Gadjah Mada2
Endah RetnaningrumUniversitas Gadjah Mada2
Nuzlan RasjidUniversitas Gadjah Mada2
Fitri NadifahUniversitas Gadjah Mada2
Fitriana PuspitasariUniversitas Gadjah Mada2
Dika SundariUniversitas Gadjah Mada2
Wayan Tunas ArtamaUniversitas Gadjah Mada2
Tri Rini NuringtyasUniversitas Gadjah Mada2
Yekti Asih PurwestriUniversitas Gadjah Mada2
Linda WidyastutiPoliteknik Kesehatan Bhakti Mulia1
Novita AndrianiMulawarman University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Early detection of the orchid flowering gene PaFT1 in tobacco cells using a GFP reporter</dc:title>
	<dc:creator>Wahyuningsih, Sri</dc:creator>
	<dc:creator>Lawrie, Muhammad Dylan</dc:creator>
	<dc:creator>Daryono, Budi Setiadi</dc:creator>
	<dc:creator>Moeljopawiro, Sukarti</dc:creator>
	<dc:creator>Jang, Soenghoe</dc:creator>
	<dc:creator>Semiarti, Endang</dc:creator>
	<dc:subject xml:lang="en-US">early flowering; GFP; PaFT1; reporter gene; tobacco</dc:subject>
	<dc:description xml:lang="en-US">Here we describe a novel method of using green fluorescence protein (GFP) as a reporter gene for early detection of an integrated T­DNA containing the orchid flowering gene, PaFT1 (Phalaenopsis aphrodite Flowering locus T1) in the tobacco genome. Functional assays that report the presence of exogenous DNA early in development are especially useful in plants where the desired phenotype is only apparent after long periods of vegetative growth. The objective of this study is to establish a method for detecting an inserted Phalaenopsis orchid flowering gene and examining its function in tobacco. The p35S::PaFT1­ 35S::GFP construct was introduced into Agrobacterium tumefaciens strain EHA101. Transformed tobacco leaves were cultured on MS medium with addition of 1 mgL-1 NAA+3 mgL-1 BAP+50 mgL-1 Kanamycin+300 mgL-1 timentin for selection. Results showed bright green GFP fluorescent signals in 11 out of 15 (73%) tobacco leaf cells at a 2­month time point after transformation. GFP and PaFT1 fragments were amplified in genomic PCR using GFP and PaFT1 specific primers. The accumulated PaFT1 transcripts were observed in 3 month­old transgenic tobacco plants containing p35S::PaFT1­35S::GFP. Green florescence was observed only in the transgenic plants at the 5 month­old stage but not in the wild type controls.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2016-06-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/26781</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.26781</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 21, No 1 (2016); 12-21</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/26781/16536</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>