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Cytotoxic activity and apoptosis induction of avocado Persea americana Mill. seed extract on MCF-7 cancer cell line

Indonesian Journal of Biotechnology · Vol. 23 No. 2 (2018) · hlm. 61–67

DOI
10.22146/ijbiotech.32141
ISSN
2089-2241, 0853-8654
Terbit
2018-12-24
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Avocado Persea Americana Mill. is a commercially important crop and studies have shown that the pulp may have benefits to cardiovascular health, dermatological health and possibly anti-cancer activity. Avocado seeds have several medicinal properties such as anti-hyperglycemic, antimicrobial, antioxidant and anti-inflammation. This study aim to evaluate the effect of avocado seed extract on viability and apoptosis of breast cancer cell line MCF-7. The anticancer effect was evaluated by cytotoxic test using MTT assay and the effect on apoptosis and cell cycle was examined by flow cytometry method. The cytotoxic test showed that chloroform extract had strong cytotoxic activity against MCF-7 cell lines with IC50 value of 94.87 µg/mL. Furthermore, the chloroform extract was partitioned with methanol and yield of soluble methanol fraction (FLM) and non soluble methanol fraction (FTLM). The cytotoxic activity of the methanol soluble fraction (FLM) and non soluble methanol fraction (FTLM) against MCF-7 cell lines was increased with IC50 of 34.52 and 66.03 µg/mL, respectively. Flow cytometry analysis using annexin-V and propidium iodide staining revealed that methanol soluble fraction could induce apoptosis and modulating the cell cycle arrest in MCF-7 cell. This research indicated that avocado seed has a potency to induce apoptosis and as anti-proliferative to MCF-7 cells lines.

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  • University of Brawijaya
  • Muhammadiyah University of Yogyakarta
  • Universitas Trisakti
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Rekan penulisOrganisasiArtikel bersama
M. MurwantokoAfiliasi tidak diketahui3
Rumiyati RumiyatiAfiliasi tidak diketahui3
Firman AlamsyahAfiliasi tidak diketahui3
Puji AstutiAfiliasi tidak diketahui2
Dyah SubositiAfiliasi tidak diketahui2
Rohmat MujahidAfiliasi tidak diketahui2
Sari HaryantiAfiliasi tidak diketahui2
Pudji AstutiUniversitas Trisakti2
Woro Anindito Sri TunjungAfiliasi tidak diketahui2
Alfi Rizqi AmaliaAfiliasi tidak diketahui2
Yekti Asih PurwestriAfiliasi tidak diketahui2
Rizal Maarif RukmanaAfiliasi tidak diketahui2
Nastiti WijayantiAfiliasi tidak diketahui2
Lilik WahyuniUniversity of Brawijaya1
Jhauharotul MuchlisyiyahUniversity of Brawijaya1
Dian Islami PrasetyaningrumUniversity of Brawijaya1
Aulia Surya SeptaniaMuhammadiyah University of Yogyakarta1
Ririn SuharsantiAfiliasi tidak diketahui1
Nunung YuniartiAfiliasi tidak diketahui1
Nina KurnianingrumAfiliasi tidak diketahui1
S. M. WidyastutiAfiliasi tidak diketahui1
Etik Erna Wati HadiAfiliasi tidak diketahui1
Dian SusantiAfiliasi tidak diketahui1
Aditya Dwi Permana PutraAfiliasi tidak diketahui1
Devi SafrinaAfiliasi tidak diketahui1
Nur Rahmawati WijayaAfiliasi tidak diketahui1
M Bakti Samsu AdiAfiliasi tidak diketahui1
Usman SiswantoAfiliasi tidak diketahui1
Rita MalizaAfiliasi tidak diketahui1
Putra SantosoAfiliasi tidak diketahui1
Bramadi AryaAfiliasi tidak diketahui1
Alimuddin TofrizalAfiliasi tidak diketahui1
Sari Budi Moria SembiringAfiliasi tidak diketahui1
Jhon Harianto HutapeaAfiliasi tidak diketahui1
I Nyoman Adiasmara GiriAfiliasi tidak diketahui1
Ketut MahardikaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Cytotoxic activity and apoptosis induction of avocado Persea americana Mill. seed extract on MCF-7 cancer cell line</dc:title>
	<dc:creator>Widiyastuti, Yuli</dc:creator>
	<dc:creator>Pratiwi, Rarastoeti</dc:creator>
	<dc:creator>Riyanto, Sugeng</dc:creator>
	<dc:creator>Wahyuono, Subagus</dc:creator>
	<dc:subject xml:lang="en-US">apoptosis; cytotoxic; MCF-7; Persea americana</dc:subject>
	<dc:description xml:lang="en-US">Avocado Persea Americana Mill. is a commercially important crop and studies have shown that the pulp may have benefits to cardiovascular health, dermatological health and possibly anti-cancer activity. Avocado seeds have several medicinal properties such as anti-hyperglycemic, antimicrobial, antioxidant and anti-inflammation. This study aim to evaluate the effect of avocado seed extract on viability and apoptosis of breast cancer cell line MCF-7. The anticancer effect was evaluated by cytotoxic test using MTT assay and the effect on apoptosis and cell cycle was examined by flow cytometry method. The cytotoxic test showed that chloroform extract had strong cytotoxic activity against MCF-7 cell lines with IC50 value of 94.87 µg/mL. Furthermore, the chloroform extract was partitioned with methanol and yield of soluble methanol fraction (FLM) and non soluble methanol fraction (FTLM). The cytotoxic activity of the methanol soluble fraction (FLM) and non soluble methanol fraction (FTLM) against MCF-7 cell lines was increased with IC50 of 34.52 and 66.03 µg/mL, respectively. Flow cytometry analysis using annexin-V and propidium iodide staining revealed that methanol soluble fraction could induce apoptosis and modulating the cell cycle arrest in MCF-7 cell. This research indicated that avocado seed has a potency to induce apoptosis and as anti-proliferative to MCF-7 cells lines.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2018-12-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/32141</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.32141</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 23, No 2 (2018); 61-67</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/32141/23711</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>