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Amylolytic ability of bacteria isolated from termite (Coptotermes sp.) gut

Indonesian Journal of Biotechnology · Vol. 23 No. 1 (2018) · hlm. 14–20

DOI
10.22146/ijbiotech.32445
ISSN
2089-2241, 0853-8654
Terbit
2018-06-11
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

BSR 2, BSR 3, BSR 8, and BSR 9, different bacteria isolated from the termite gut, have been shown to possess cellulolytic activities, but their amylolytic ability has heretofore been unknown. This study attempted to fill in this knowledge gap. The formation of a clear zone using the iodine test showed that the bacteria were able to produce and secrete amylase. Based on the results, the best cultivation times for strains BSR 2, BSR 3, BSR 8, and BSR 9 were 6, 3, 2, and 2 d, respectively, yielding amylase activities of 2.59 ± 0.13 U/mg, 2.00 ± 0.08 U/mg, 1.67 ± 0.10 U/mg, and 1.55 ± 0.12 U/mg, respectively. BSR 2 had the highest amylase activity compared with the other bacterial isolates. The optimum ph for bacterial amylase activity of BSR 2 was 7.0, and the optimum temperature was 40°C. The molecular characterization of isolates BSR 2, BSR 3, BSR 8, and BSR 9 was based on 16S rRNA gene sequences. Isolates BSR 8 and BSR 9 were thus identified as Brevibacillus parabrevis and Brevibacillus sp. With similarities amounting to 92.48% and 95.91%, while the BSR 3 isolate was identified as Pseudomonas alcaligenes with a similarity of 94.29%, and the BSR 2 isolate could not be identified yet.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Universitas Muhammadiyah Pontianak
  • Universitas Islam Negeri Sultan Maulana Hasanuddin Banten
  • Universitas Bangun Nusantara
  • Institut Pertanian Stiper
  • Tokyo University of Agriculture and Technology
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  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Ardaning NurilianiUniversitas Gadjah Mada4
Woro Anindito Sri TunjungUniversitas Gadjah Mada4
Langkah SembiringUniversitas Gadjah Mada3
Didik IndradewaUniversitas Gadjah Mada3
Ghea Putri ChristyAfiliasi tidak diketahui3
Charlie Ester de FretesNational Research and Innovation Agency2
Rarastoeti PratiwiUniversitas Gadjah Mada2
Tri Rini NuringtyasUniversitas Gadjah Mada2
Donny WidiantoUniversitas Gadjah Mada2
Alfino SebastianUniversitas Gadjah Mada2
Wahyu Aristyaning PutriUniversitas Gadjah Mada2
Hanum Mukti RahayuUniversitas Muhammadiyah Pontianak2
Anis Uswatun KhasanahUniversitas Islam Negeri Sultan Maulana Hasanuddin Banten2
Yuni SineUniversitas Gadjah Mada2
Taryono TaryonoUniversitas Gadjah Mada2
Ratna Dewi EskundariUniversitas Bangun Nusantara2
Bima MahendraUniversitas Gadjah Mada1
Nabila Ramiza PuteriUniversitas Gadjah Mada1
Sekar RahayuUniversitas Gadjah Mada1
Yuka OgakiAfiliasi tidak diketahui1
Hiroyuki TsujiAfiliasi tidak diketahui1
Ko ShimamotoAfiliasi tidak diketahui1
Kumala DewiUniversitas Gadjah Mada1
Yohana Theresia Maria AstutiInstitut Pertanian Stiper1
Lila NatasaputraAfiliasi tidak diketahui1
P. ParmiUniversitas Gadjah Mada1
Arnia Sari MukaromahUniversitas Gadjah Mada1
Yoshiharu FujiiTokyo University of Agriculture and Technology1
Pratiwi ApridamayantiTanjungpura University1
Rumiyati RumiyatiUniversitas Gadjah Mada1
Dyah IsmoyowatiUniversitas Gadjah Mada1
Dio N. WijayaUniversitas Gadjah Mada1
Febri Adi SusantoUniversitas Gadjah Mada1
Ratna Stia DewiJenderal Soedirman University1
Rina Sri KasiamdariUniversitas Gadjah Mada1
Erni MartaniUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Amylolytic ability of bacteria isolated from termite (Coptotermes sp.) gut</dc:title>
	<dc:creator>Mulyani, Putri Dwi</dc:creator>
	<dc:creator>Hamid, Radhiyah Mardhiyah</dc:creator>
	<dc:creator>Janatunaim, Rifqi Zahroh</dc:creator>
	<dc:creator>Purwestri, Yekti Asih</dc:creator>
	<dc:subject xml:lang="en-US">16 rRNA gene; amylase; amylolytic bacteria; termite gut</dc:subject>
	<dc:description xml:lang="en-US">BSR 2, BSR 3, BSR 8, and BSR 9, different bacteria isolated from the termite gut, have been shown to possess cellulolytic activities, but their amylolytic ability has heretofore been unknown. This study attempted to fill in this knowledge gap. The formation of a clear zone using the iodine test showed that the bacteria were able to produce and secrete amylase. Based on the results, the best cultivation times for strains BSR 2, BSR 3, BSR 8, and BSR 9 were 6, 3, 2, and 2 d, respectively, yielding amylase activities of 2.59 ± 0.13 U/mg, 2.00 ± 0.08 U/mg, 1.67 ± 0.10 U/mg, and 1.55 ± 0.12 U/mg, respectively. BSR 2 had the highest amylase activity compared with the other bacterial isolates. The optimum ph for bacterial amylase activity of BSR 2 was 7.0, and the optimum temperature was 40°C. The molecular characterization of isolates BSR 2, BSR 3, BSR 8, and BSR 9 was based on 16S rRNA gene sequences. Isolates BSR 8 and BSR 9 were thus identified as Brevibacillus parabrevis and Brevibacillus sp. With similarities amounting to 92.48% and 95.91%, while the BSR 3 isolate was identified as Pseudomonas alcaligenes with a similarity of 94.29%, and the BSR 2 isolate could not be identified yet.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2018-06-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/32445</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.32445</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 23, No 1 (2018); 14-20</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/32445/21228</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>