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Use of microsatellite markers to detect heterozygosity in an F2 generation of a black rice and white rice cross

Indonesian Journal of Biotechnology · Vol. 23 No. 1 (2018) · hlm. 28–34

DOI
10.22146/ijbiotech.33111
ISSN
2089-2241, 0853-8654
Terbit
2018-06-11
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The aim of this research was to know the heterozygosity of F2 generation from black rice and white rice crossing using microsatellite marker. The research material consisted of F2 Sx G plant population from black rice (S) and white rice Situbagendit (G) crosses, female parent of black rice (S), male parent of white rice (G), chemical and organic fertilizer, chemicals and tools for molecular activity and 3 microsatellite markers related to color properties (RM 220, RM 224 and RM 252). All of plant populations (generation F2, parent female, parent male) were planted in fields up to harvest. Young leaves (30 days after planting) all of plant populations were molecularly analyzed using 3 microsatellite markers (RM 220, RM 224 and RM 252). Stages of this activity include DNA isolation, PCR reaction, and visualization of PCR results using Metaphore Agarose Gel Electrophoresis. The results showed that the percentage of the number of individual plants showing heterozygous pattern in F2 S × G plant generation was 50% (RM 220); 40% (RM 224) and 60% (RM 252), so the RM 252 microsatellite marker was effectively used as a DNA-assisted selection tool on the crossbreed of black rice with white rice.

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Rani Agustina WulandariAfiliasi tidak diketahui3
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Yunus Pajanjan PaulanganCenderawasih University1
Muh. Arsyad Al. AminIPB University1
Barnabas BarapadangCenderawasih University1
Yudi WahyudinIPB University1
Mahfud MahfudAfiliasi tidak diketahui1
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Eko HanudinAfiliasi tidak diketahui1
Dewi Ratna NurhayatiAfiliasi tidak diketahui1
Yuli PriyanaAfiliasi tidak diketahui1
Elisa AnggraeniIPB University1
Machfud MachfudIPB University1
Marimin MariminIPB University1
Raden Bagus Tri Joko WibowoAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Use of microsatellite markers to detect heterozygosity in an F2 generation of a black rice and white rice cross</dc:title>
	<dc:creator>Kristamtini, Kristamtini</dc:creator>
	<dc:creator>Taryono, Taryono</dc:creator>
	<dc:creator>Basunanda, Panjisakti</dc:creator>
	<dc:creator>Murti, Rudi Hari</dc:creator>
	<dc:subject xml:lang="en-US">black rice; F2 generation; heterozygosity; microsatellite</dc:subject>
	<dc:description xml:lang="en-US">The aim of this research was to know the heterozygosity of F2  generation from black rice and white rice crossing using microsatellite marker.  The research material consisted of F2 Sx G plant population from black rice (S) and white rice Situbagendit (G) crosses, female parent of black rice (S), male parent of white rice (G), chemical and organic fertilizer, chemicals and tools for molecular activity and 3 microsatellite markers related to color properties  (RM 220, RM 224 and RM 252). All of plant populations (generation F2, parent female, parent male) were planted in fields up to harvest. Young leaves (30 days after planting) all of plant populations were molecularly analyzed using 3 microsatellite markers (RM 220, RM 224 and RM 252). Stages of this activity include DNA isolation, PCR reaction, and visualization of PCR results using Metaphore Agarose Gel Electrophoresis. The results showed that the percentage of the number of individual plants showing heterozygous pattern in F2 S × G plant generation was 50% (RM 220); 40% (RM 224) and 60% (RM 252), so the RM 252 microsatellite marker was effectively used as a DNA-assisted selection tool on the crossbreed of black rice with white rice.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Assessment Institute for Agricultural Technology Yogyakarta</dc:contributor>
	<dc:date>2018-06-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/33111</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.33111</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 23, No 1 (2018); 28-34</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/33111/21223</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>