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Identification of single nucleotide polymorphisms in GDF9 gene associated with litter size in Garut sheep

Indonesian Journal of Biotechnology · Vol. 24 No. 1 (2019) · hlm. 51–56

DOI
10.22146/ijbiotech.42095
ISSN
2089-2241, 0853-8654
Terbit
2019-06-26
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The growth differentiation factor 9 (GDF9) gene has been regarded as having major impacts on ovulation rate and litter size in sheep. The aim of this study was to identify the single nucleotide polymorphisms (SNPs) of the GDF9 gene and their association with litter size in Garut sheep. For this purpose, a total of 60 ewes of Garut sheep were included in this study. Based on the sheep GDF9 reference sequences (Genbank Acc. No. AF078545.2), one pair of primers (5’-CTGCTGTTTAACCTGGATCGTG-3 5’-GGAGAGCCATACCGATGTCC-3 as forward and reverse, respectively) was used for PCR amplification. The results revealed that four SNPs (g.54C>T, g.60G>A, g.304G>A, and g.333G>A) were found in Garut sheep by direct sequencing. For SNP g.54C>T, the sheep exhibited the highest frequency of allele C and genotype CC. On the other hand, SNPs g.60G>A, g.304G>A, and g.333G>A showed a higher frequency of allele G than allele A, and the GG genotype was predominant in the population. SNP g.333G>A had a significant effect on litter size (p A may be useful as a genetic marker for litter size in Garut sheep.

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  • State University of Semarang
  • Cenderawasih University
  • Universitas Negeri Surabaya
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Naila Rofi’atiAfiliasi tidak diketahui1
Lina HerlinaAfiliasi tidak diketahui1
Nugroho Edi KartijonoState University of Semarang1
Riskawati YuanitaAfiliasi tidak diketahui1
Wiwi IsnaeniState University of Semarang1
Ai AstutiAfiliasi tidak diketahui1
Amin RetnoningsihAfiliasi tidak diketahui1
Idaliani PrikadiastutiAfiliasi tidak diketahui1
Saiful RidloState University of Semarang1
Muhammad IrhasAfiliasi tidak diketahui1
Evita AnggereiniAfiliasi tidak diketahui1
Upik YeliantiAfiliasi tidak diketahui1
Sarah Sakinah UmadiAfiliasi tidak diketahui1
Denny Sobardini SobarnaAfiliasi tidak diketahui1
Mieke Rochimi SetiawatiAfiliasi tidak diketahui1
Anne NurainiAfiliasi tidak diketahui1
Benang - PurwantoAfiliasi tidak diketahui1
Indrawati - IndrawatiAfiliasi tidak diketahui1
Tualar SimarmataAfiliasi tidak diketahui1
Yuyun YuwariahAfiliasi tidak diketahui1
Purwanto PurwantoCenderawasih University1
Dodi SukmayanaAfiliasi tidak diketahui1
Kovertina Rakhmi IndrianaAfiliasi tidak diketahui1
Cucu SuhermanAfiliasi tidak diketahui1
Santi RosniawatyAfiliasi tidak diketahui1
W. WidodoUniversitas Negeri Surabaya1
Slamet Diah VolkandariAfiliasi tidak diketahui1
S. SumadiAfiliasi tidak diketahui1
Sulthon SulthonAfiliasi tidak diketahui1
I Nyoman S. DegengAfiliasi tidak diketahui1
Waras WarasAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Identification of single nucleotide polymorphisms in GDF9 gene associated with litter size in Garut sheep</dc:title>
	<dc:creator>Rahmawati, Resti Yuliana</dc:creator>
	<dc:creator>Sumadi, Sumadi</dc:creator>
	<dc:creator>Hartatik, Tety</dc:creator>
	<dc:subject xml:lang="en-US">Garut sheep; GDF9 gene; prolificacy; single nucleotide polymorphism (SNP)</dc:subject>
	<dc:description xml:lang="en-US">The growth differentiation factor 9 (GDF9) gene has been regarded as having major impacts on ovulation rate and litter size in sheep. The aim of this study was to identify the single nucleotide polymorphisms (SNPs) of the GDF9 gene and their association with litter size in Garut sheep. For this purpose, a total of 60 ewes of Garut sheep were included in this study. Based on the sheep GDF9 reference sequences (Genbank Acc. No. AF078545.2), one pair of primers (5’-CTGCTGTTTAACCTGGATCGTG-3 5’-GGAGAGCCATACCGATGTCC-3 as forward and reverse, respectively) was used for PCR amplification. The results revealed that four SNPs (g.54C&gt;T, g.60G&gt;A, g.304G&gt;A, and g.333G&gt;A) were found in Garut sheep by direct sequencing. For SNP g.54C&gt;T, the sheep exhibited the highest frequency of allele C and genotype CC. On the other hand, SNPs g.60G&gt;A, g.304G&gt;A, and g.333G&gt;A showed a higher frequency of allele G than allele A, and the GG genotype was predominant in the population. SNP g.333G&gt;A had a significant effect on litter size (p &lt; 0.05), and ewes with the GG genotype had a higher litter size than those with the GA genotype. Genotype distributions for all identified SNPs were in agreement with Hardy-Weinberg equilibrium. We highlight that SNP g.333G&gt;A may be useful as a genetic marker for litter size in Garut sheep.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">The study was supported by postgraduate program, Faculty of Animal Science, Universitas Gadjah Mada. Also, we thank Retno Setyawati for helping DNA analysis.</dc:contributor>
	<dc:date>2019-06-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/42095</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.42095</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 24, No 1 (2019); 51-56</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/42095/24784</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>