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Diagnosis and molecular characterization of Anaplasma platys in dog patients in Yogyakarta area, Indonesia

Indonesian Journal of Biotechnology · Vol. 24 No. 1 (2019) · hlm. 43–50

DOI
10.22146/ijbiotech.42750
ISSN
2089-2241, 0853-8654
Terbit
2019-06-18
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Anaplasma platys is a tick-borne, Gram-negative bacterium that causes anaplasmosis, a companion vector-borne disease impacting dogs. Information on this disease remains limited in Indonesia. Its symptoms are not specific, so molecular analysis is required for a rapid and accurate diagnosis. GroEL is an essential gene commonly used for classification and species identification of many groups of bacteria, including Anaplasma spp. In this study, a molecular diagnosis of anaplasmosis based on the groEL gene sequence was conducted using PCR. In addition, the genetic diversity of Anaplasma platys in infected dogs was determined. Blood samples were collected from 51 dogs suspected of anaplasmosis from Prof. Dr. Soeparwi Animal Hospital, animal clinics, and pet shops in the Yogyakarta area, Indonesia, based on anamnesis, histories of tick infestations, and clinical symptom examinations. DNA extraction and PCR targeting the groEL gene were performed, followed by sequencing. Phylogenetic tree analysis and construction were carried out using the BLAST and MEGA programs. Positive PCR sample results (amplicon length of 624 bp) were found in 6 of 51 dogs. Samples A1 (KHJ/C2), A2 (KHJ/A2), A3 (KSK/L), A4 (KHJ/L), and A5 (KNP/M2) had close ties to Anaplasma platys (AF478129.1) from GenBank. Phylogenetic analysis showed a very high homology value (100%) and bootstrap value of 100%. It can be concluded that there was no genetic diversity in the Anaplasma platys found in infected dogs in the Yogyakarta area.

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  • Universitas Gadjah Mada
  • Tadulako University
  • Justus-Liebig-Universität Gießen
  • University of Würzburg
  • Chulalongkorn University
  • Universitas Sulawesi Barat
  • Atma Jaya Catholic University of Indonesia
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Rekan penulisOrganisasiArtikel bersama
Nastiti WijayantiUniversitas Gadjah Mada7
Michael KannJustus-Liebig-Universität Gießen4
Warih Pulung NugrahaniAfiliasi tidak diketahui3
Diana SavitriUniversitas Gadjah Mada2
Irhamna PutriAfiliasi tidak diketahui2
Medania PurwaningrumUniversitas Gadjah Mada2
Hevi WihadmadyatamiUniversitas Gadjah Mada2
Krisna Noli AndrianUniversitas Gadjah Mada2
Tri WibawaUniversitas Gadjah Mada1
Hera NirwatiUniversitas Gadjah Mada1
Sp.A SutaryoUniversitas Gadjah Mada1
Nenny Sri MulyaniAfiliasi tidak diketahui1
Titis Budi WidowatiAfiliasi tidak diketahui1
Purnomo HadiAfiliasi tidak diketahui1
Ratu Fresa Khoirotunnisa HidayatUniversitas Gadjah Mada1
Irhamna Putri RahmawatiAfiliasi tidak diketahui1
Inggit Nindika Dianing RatriUniversitas Gadjah Mada1
Dela Ria NestiUniversitas Gadjah Mada1
Ahmad BaidlowiAfiliasi tidak diketahui1
Achmad FauziAfiliasi tidak diketahui1
Balqis Bahiya Milan NovindasariUniversitas Gadjah Mada1
Isti Ananda NurrahmiUniversitas Gadjah Mada1
Srikanth KarnatiUniversity of Würzburg1
Yohanna KayanavedaUniversitas Gadjah Mada1
Chenphop SawangmakeChulalongkorn University1
Hendro SukocoUniversitas Sulawesi Barat1
Ferbian Milas SiswantoAtma Jaya Catholic University of Indonesia1
Agung BudiyantoUniversitas Gadjah Mada1
Erif Maha Nugraha SetyawanUniversitas Gadjah Mada1
SuriansyahTadulako University1
Annisa Putri CahyaniAfiliasi tidak diketahui1
SalminTadulako University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Diagnosis and molecular characterization of Anaplasma platys in dog patients in Yogyakarta area, Indonesia</dc:title>
	<dc:creator>Faizal, Muh. Disna</dc:creator>
	<dc:creator>Haryanto, Aris</dc:creator>
	<dc:creator>Tjahajati, Ida</dc:creator>
	<dc:subject xml:lang="en-US">Anaplasma platys; companion vector-borne disease (CVBD); groEL gene; phylogenetic analysis; polymerase chain reaction</dc:subject>
	<dc:description xml:lang="en-US">Anaplasma platys is a tick-borne, Gram-negative bacterium that causes anaplasmosis, a companion vector-borne disease impacting dogs. Information on this disease remains limited in Indonesia. Its symptoms are not specific, so molecular analysis is required for a rapid and accurate diagnosis. GroEL is an essential gene commonly used for classification and species identification of many groups of bacteria, including Anaplasma spp. In this study, a molecular diagnosis of anaplasmosis based on the groEL gene sequence was conducted using PCR. In addition, the genetic diversity of Anaplasma platys in infected dogs was determined. Blood samples were collected from 51 dogs suspected of anaplasmosis from Prof. Dr. Soeparwi Animal Hospital, animal clinics, and pet shops in the Yogyakarta area, Indonesia, based on anamnesis, histories of tick infestations, and clinical symptom examinations. DNA extraction and PCR targeting the groEL gene were performed, followed by sequencing. Phylogenetic tree analysis and construction were carried out using the BLAST and MEGA programs. Positive PCR sample results (amplicon length of 624 bp) were found in 6 of 51 dogs. Samples A1 (KHJ/C2), A2 (KHJ/A2), A3 (KSK/L), A4 (KHJ/L), and A5 (KNP/M2) had close ties to Anaplasma platys (AF478129.1) from GenBank. Phylogenetic analysis showed a very high homology value (100%) and bootstrap value of 100%. It can be concluded that there was no genetic diversity in the Anaplasma platys found in infected dogs in the Yogyakarta area.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">2018 Faculty Competitive Research Faculty of Veterinary Medicine, Universitas Gadjah Mada, No. 1584/J01.1.22/HK4/2018</dc:contributor>
	<dc:date>2019-06-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/42750</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.42750</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 24, No 1 (2019); 43-50</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/42750/24731</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>