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Repetitive DNA sequences accelerate molecular cytogenetic research in plants with small chromosomes

Indonesian Journal of Biotechnology · Vol. 24 No. 2 (2019) · hlm. 82–87

DOI
10.22146/ijbiotech.51726
ISSN
2089-2241, 0853-8654
Terbit
2019-12-05
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Repetitive DNA sequences are highly abundant in plant genomes and are favorable probes for chromosome identification in plants. However, it is difficult to conduct studies on the details of metaphase chromosome structures in plants with small chromosomes due to their highly condensed status. Therefore, identification of homologous chromosomes for karyotyping and analyzing chromosome structures is a challenging issue for cytogeneticists without specific probes and precise chromosome stages. In this study, five repetitive DNA probes, i.e., 5S and 45S ribosomal DNAs (rDNAs), melon centromeric sequence (Cmcent), cucumber subtelomeric sequence (Type I), and microsatellite (CT)10 repeats, were used to identify primary constrictions and homologous chromosomes for karyotyping. Four and two loci of 45S rDNA were respectively observed on metaphase and pachytene chromosomes of Abelia × grandiflora. Cmcent was detected on both primary constrictions of melon pachytene and metaphase chromosomes. Furthermore, one pair of 5S rDNA signals were hybridized on melon metaphase chromosomes. Eight and two loci of 45S and 5S rDNA were respectively detected on cucumber chromosomes. Type I and (CT)10 probes were specifically hybridized on subtelomeric and interstitial regions on the chromosomes, respectively. These results suggest that repetitive DNA sequences are versatile probes for chromosome identification in plants with small chromosomes, particularly for karyotyping analyses.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Chiba University
  • UIN Raden Mas Said Surakarta
  • Indonesian Coffee and Cocoa Research Institute
  • University of Malaya
  • Universiti Sains Malaysia
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Aziz PurwantoroUniversitas Gadjah Mada8
Dyah Weny RespatieUniversitas Gadjah Mada4
Binti Nur AisahUniversitas Gadjah Mada2
Aprilia Tiara IkhwaniUniversitas Gadjah Mada2
Erlina AmbarwatiUniversitas Gadjah Mada2
Listy AnggraeniNational Research and Innovation Agency2
Chandra SetyawanUniversitas Gadjah Mada2
Aniswatul KhamidahNational Research and Innovation Agency2
Ilyas IlyasUniversitas Syiah Kuala2
Yeni FatmawatiUniversitas Gadjah Mada2
Tsalatsatun Nur HidayahUIN Raden Mas Said Surakarta1
Adillah Khairun Nisa’UIN Raden Mas Said Surakarta1
Michael Hagana BangunAfiliasi tidak diketahui1
B. SALLEH B. SALLEHAfiliasi tidak diketahui1
L. JULIAAfiliasi tidak diketahui1
A. SAFINATUniversiti Sains Malaysia1
Irna AuliauzzakiaUniversitas Gadjah Mada1
Rikcy Setiaji NugrahaUniversitas Gadjah Mada1
Sairoh Bisirotil MujtabaUniversitas Gadjah Mada1
Aditya Hanung SetyadiUniversitas Gadjah Mada1
SuhardiAfiliasi tidak diketahui1
Widya Pintaka Bayu PutraNational Research and Innovation Agency1
Pijug SummpunnWalailak University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Repetitive DNA sequences accelerate molecular cytogenetic research in plants with small chromosomes</dc:title>
	<dc:creator>Setiawan, Agus Budi</dc:creator>
	<dc:creator>Wibowo, Ari</dc:creator>
	<dc:creator>Teo, Chee How</dc:creator>
	<dc:creator>Kikuchi, Shinji</dc:creator>
	<dc:creator>Koba, Takato</dc:creator>
	<dc:subject xml:lang="en-US">homologous chromosomes; microsatellite repeats; pachytene chromosomes; precise karyotyping; repetitive DNAs</dc:subject>
	<dc:description xml:lang="en-US">Repetitive DNA sequences are highly abundant in plant genomes and are favorable probes for chromosome identification in plants. However, it is difficult to conduct studies on the details of metaphase chromosome structures in plants with small chromosomes due to their highly condensed status. Therefore, identification of homologous chromosomes for karyotyping and analyzing chromosome structures is a challenging issue for cytogeneticists without specific probes and precise chromosome stages. In this study, five repetitive DNA probes, i.e., 5S and 45S ribosomal DNAs (rDNAs), melon centromeric sequence (Cmcent), cucumber subtelomeric sequence (Type I), and microsatellite (CT)10 repeats, were used to identify primary constrictions and homologous chromosomes for karyotyping. Four and two loci of 45S rDNA were respectively observed on metaphase and pachytene chromosomes of Abelia × grandiflora. Cmcent was detected on both primary constrictions of melon pachytene and metaphase chromosomes. Furthermore, one pair of 5S rDNA signals were hybridized on melon metaphase chromosomes. Eight and two loci of 45S and 5S rDNA were respectively detected on cucumber chromosomes. Type I and (CT)10 probes were specifically hybridized on subtelomeric and interstitial regions on the chromosomes, respectively. These results suggest that repetitive DNA sequences are versatile probes for chromosome identification in plants with small chromosomes, particularly for karyotyping analyses.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2019-12-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/51726</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.51726</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 24, No 2 (2019); 82-87</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/51726/26456</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51726/10096</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51726/10097</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51726/10098</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51726/10099</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51726/10100</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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