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Cytoprotective activity of carrot and tomato callus extracts and the ex‐ pression of cytokines in UV‐B irradiated fibroblast cells

Indonesian Journal of Biotechnology · Vol. 24 No. 2 (2020) · hlm. 94–100

DOI
10.22146/ijbiotech.51734
ISSN
2089-2241, 0853-8654
Terbit
2020-01-02
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Studies have suggested that both carrot (Daucus carota L.) and tomato (Solanum lycopersicum L.) callus extracts contain antoxidant compounds that might have the potental to protect cells from free radicals such as H2O2 that contribute to cell damage. The other sources of free radical exposure in human cells, such as UV‐B, should also be examined. UV‐B exposure can trigger increased expression of inflammatory cytokines such as cyclooxygenase‐2 (COX‐2) and tumor necrosis factor‐α (TNF‐α) and the antinflammatory cytokine interleukin‐10 (IL‐10), which causes photoaging. This study was conducted to investigate the cytoprotectve actvity of carrot and tomato callus aqueous extracts by observing cell viability using the MTT assay. Immunocytochemistry methods were used to examine the effects of carrot and tomato callus aqueous extracts on the expression of COX‐2, TNF‐α, and IL‐10 in human dermal fibroblast adult (HDFa) cells exposed to UV‐B light. Carrot and tomato callus aqueous extracts were obtained by the maceration method using aqua bidistilled solvent. Results showed that both carrot and tomato callus aqueous extracts at 0.5 mg/mL exhibited the highest cytoprotective effect in HDFa cells compared to that at other concentratons. Both carrot and tomato callus aqueous extracts could also decrease the expression of COX‐2 and TNF‐α, whereas carrot callus aqueous extract increased the expression of the anti‐inflammatory cytokine IL‐10 in HDFa cells.

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  • Universitas Gadjah Mada
  • Universitas Pattimura
  • University of Warwick
  • Diponegoro University
  • Cenderawasih University
  • Universitas Muhammadiyah Semarang
  • Jenderal Soedirman University
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Rekan penulisOrganisasiArtikel bersama
Aziz PurwantoroUniversitas Gadjah Mada11
Sukarti MoeljopawiroUniversitas Gadjah Mada6
Ari IndriantoUniversitas Gadjah Mada4
Muhammad Dylan LawrieUniversitas Gadjah Mada4
Windi MoseUniversitas Pattimura3
Febri Yuda KurniawanUniversitas Gadjah Mada3
Rarastoeti PratiwiUniversitas Gadjah Mada3
Jose Gutierrez‐MarcosUniversity of Warwick3
Nintya SetiariDiponegoro University2
Jaka WidadaUniversitas Gadjah Mada2
Budi Setiadi DaryonoUniversitas Gadjah Mada2
Aviesta LinggabuwanaUniversitas Gadjah Mada2
Saifa Usni PutriUniversitas Gadjah Mada2
Nuzlan RasjidUniversitas Gadjah Mada2
Exsyupransia MursyantiUniversitas Gadjah Mada2
Fitriana PuspitasariUniversitas Gadjah Mada2
Riris Istighfari JenieUniversitas Gadjah Mada2
Aries Bagus SasongkoUniversitas Gadjah Mada2
Naufal Ghozi Aditya PerdanaUniversitas Gadjah Mada2
Dika SundariUniversitas Gadjah Mada2
Yekti Asih PurwestriUniversitas Gadjah Mada2
Woro Anindito Sri TunjungUniversitas Gadjah Mada2
Aditya Krishar KarimCenderawasih University1
Tri Joko RaharjoState University of Semarang1
Stalis Norma EthicaUniversitas Muhammadiyah Semarang1
Oedjijono OedjijonoJenderal Soedirman University1
Triono Bagus SaputroInstitut Teknologi Sepuluh Nopember1
Zulfa LayinaUniversitas Gadjah Mada1
Della Rosiana NingtiasUniversitas Gadjah Mada1
Falah Nur AlifiantoUniversitas Gadjah Mada1
Rina ArimarsetiowatiIndonesian Coffee and Cocoa Research Institute1
Yohana Theresia Maria AstutiInstitut Pertanian Stiper1
Ana AininaUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Cytoprotective activity of carrot and tomato callus extracts and the ex‐ pression of cytokines in UV‐B irradiated fibroblast cells</dc:title>
	<dc:creator>Rumiyati, Rumiyati</dc:creator>
	<dc:creator>Sismindari, Sismindari</dc:creator>
	<dc:creator>Semiarti, Endang</dc:creator>
	<dc:creator>Widyarani, Sitarina</dc:creator>
	<dc:creator>Sari, Dewi Tika</dc:creator>
	<dc:creator>Apritadila, Brilliant Kharisma</dc:creator>
	<dc:creator>Riastri, Anami</dc:creator>
	<dc:subject xml:lang="en-US">carrot (Daucus carota L.) and tomato (Solanum lycopersicum L.) callus aqueous extract; viability cell; cyclooxygenase‐2 (COX‐2); tumor necrosis factor‐α (TNF‐α); interleukin‐10 (IL‐10)</dc:subject>
	<dc:description xml:lang="en-US">Studies have suggested that both carrot (Daucus carota L.) and tomato (Solanum lycopersicum L.) callus extracts contain antoxidant compounds that might have the potental to protect cells from free radicals such as H2O2 that contribute to cell damage. The other sources of free radical exposure in human cells, such as UV‐B, should also be examined. UV‐B exposure can trigger increased expression of inflammatory cytokines such as cyclooxygenase‐2 (COX‐2) and tumor necrosis factor‐α (TNF‐α) and the antinflammatory cytokine interleukin‐10 (IL‐10), which causes photoaging. This study was conducted to investigate the cytoprotectve actvity of carrot and tomato callus aqueous extracts by observing cell viability using the MTT assay. Immunocytochemistry methods were used to examine the effects of carrot and tomato callus aqueous extracts on the expression of COX‐2, TNF‐α, and IL‐10 in human dermal fibroblast adult (HDFa) cells exposed to UV‐B light. Carrot and tomato callus aqueous extracts were obtained by the maceration method using aqua bidistilled solvent. Results showed that both carrot and tomato callus aqueous extracts at 0.5 mg/mL exhibited the highest cytoprotective effect in HDFa cells compared to that at other concentratons. Both carrot and tomato callus aqueous extracts could also decrease the expression of COX‐2 and TNF‐α, whereas carrot callus aqueous extract increased the expression of the anti‐inflammatory cytokine IL‐10 in HDFa cells. </dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2020-01-02</dc:date>
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	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/51734</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.51734</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 24, No 2 (2019); 94-100</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/51734/26700</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10112</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10113</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10114</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10115</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10116</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10117</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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