Akses terbuka
Jurnal
SINTA 1
CRISPR/Cas9‐mediated knockout of an oil palm defense‐related gene to the pathogenic fungus Ganoderma boninense
Indonesian Journal of Biotechnology
ISSN 2089-2241, 0853-8654
Terbit 2020-01-02
Penerbit Universitas Gadjah Mada
Bahasa eng
Abstrak
Oil palm plantation in Indonesia is significantly affected by basal stem rot disease caused by the pathogenic fungus Ganoderma boninense. Tolerant oil palm cultivars toward G. boninense have been developed through a breeding program accelerated by the implementation of the CRISPR/Cas9 technology. This study was conducted to perform a gene knockout (KO) of oil palm that confers a putative defense‐related trait toward G. boninense. A plasmid pCRISPR_EMLP containing modules, i.e., 35S‐CaMV‐promoter‐driven CRISPR/Cas9, U6‐promoter‐driven sgRNA to the target EgEMLP gene (EL695076), and hygromycin resistance gene as the selectable marker, was established for Agrobacterium‐mediated delivery into oil palm calli (OPC). The transformed OPCs were regenerated and screened in DF (de Fossard) media containing hygromycin. The working concentration of hygromycin was successfully optimized for selection at 20 ppm. Through PCR‐based selection using HYG primers, we succeeded in discerning positive transformed OPC clones. The sequenced PCR products of genomic DNA as the template amplified using EMLP1 primers showed a point mutation, causing a frameshift in the edited EgEMLP and premature stop codon. Furthermore, in silico modeling demonstrated that the mutation resulted in a change in the C‐terminal region, affecting the tertiary protein structure. Moreover, electrophoresis analysis of PCR products of cDNA as the template from transformed OPC clones showed several samples with faint or undetected bands. This indicated that the CRISPR/Cas9 module induced a mutation that could destabilize the transcribed mRNA, e.g., premature degradation. Altogether, this study has successfully implemented CRISPR/Cas9 gene editing in oil palm in a model gene that is responsible for putative defense‐related traits toward the pathogenic fungus G. boninense.
Jaringan kolaborasi para penulis
28 penulis ·
101 tautan kepenulisan bersama
· 41 lintas organisasi
Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.
Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.
Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
<dc:title xml:lang="en-US">CRISPR/Cas9‐mediated knockout of an oil palm defense‐related gene to the pathogenic fungus Ganoderma boninense</dc:title>
<dc:creator>Budiani, Asmini</dc:creator>
<dc:creator>Nugroho, Imam Bagus</dc:creator>
<dc:creator>Sari, Dini Astika</dc:creator>
<dc:creator>Palupi, Inez</dc:creator>
<dc:creator>Putranto, Riza Arief</dc:creator>
<dc:subject xml:lang="en-US">gene editing; plant‐microbe interaction; Ganoderma boninense; defense trait; SNP</dc:subject>
<dc:description xml:lang="en-US">Oil palm plantation in Indonesia is significantly affected by basal stem rot disease caused by the pathogenic fungus Ganoderma boninense. Tolerant oil palm cultivars toward G. boninense have been developed through a breeding program accelerated by the implementation of the CRISPR/Cas9 technology. This study was conducted to perform a gene knockout (KO) of oil palm that confers a putative defense‐related trait toward G. boninense. A plasmid pCRISPR_EMLP containing modules, i.e., 35S‐CaMV‐promoter‐driven CRISPR/Cas9, U6‐promoter‐driven sgRNA to the target EgEMLP gene (EL695076), and hygromycin resistance gene as the selectable marker, was established for Agrobacterium‐mediated delivery into oil palm calli (OPC). The transformed OPCs were regenerated and screened in DF (de Fossard) media containing hygromycin. The working concentration of hygromycin was successfully optimized for selection at 20 ppm. Through PCR‐based selection using HYG primers, we succeeded in discerning positive transformed OPC clones. The sequenced PCR products of genomic DNA as the template amplified using EMLP1 primers showed a point mutation, causing a frameshift in the edited EgEMLP and premature stop codon. Furthermore, in silico modeling demonstrated that the mutation resulted in a change in the C‐terminal region, affecting the tertiary protein structure. Moreover, electrophoresis analysis of PCR products of cDNA as the template from transformed OPC clones showed several samples with faint or undetected bands. This indicated that the CRISPR/Cas9 module induced a mutation that could destabilize the transcribed mRNA, e.g., premature degradation. Altogether, this study has successfully implemented CRISPR/Cas9 gene editing in oil palm in a model gene that is responsible for putative defense‐related traits toward the pathogenic fungus G. boninense.</dc:description>
<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
<dc:contributor xml:lang="en-US"></dc:contributor>
<dc:date>2020-01-02</dc:date>
<dc:type>info:eu-repo/semantics/article</dc:type>
<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
<dc:type xml:lang="en-US">Original Research Articles</dc:type>
<dc:format>application/pdf</dc:format>
<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/52170</dc:identifier>
<dc:identifier>10.22146/ijbiotech.52170</dc:identifier>
<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 24, No 2 (2019); 101-105</dc:source>
<dc:source>2089-2241</dc:source>
<dc:source>0853-8654</dc:source>
<dc:language>eng</dc:language>
<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/52170/26701</dc:relation>
<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/52170/10277</dc:relation>
<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/52170/10279</dc:relation>
<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/52170/10280</dc:relation>
<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/52170/10281</dc:relation>
<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/52170/10282</dc:relation>
<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/52170/10283</dc:relation>
<dc:rights xml:lang="en-US">Copyright (c) 2020 The Author(s)</dc:rights>
<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>
Sitasi dan pengindeksan
Jumlah menurut tiap indeks pada 13 Nov 2026; setiap indeks menghitung sitasi dengan cara berbeda.
OpenAlex juga mencatat karya ini di: crossref, doaj.
Sitasi artikel ini
APA 7
Budiani, A., Nugroho, I. B., Sari, D. A., Palupi, I., & Putranto, R. A. (2020). CRISPR/Cas9‐mediated knockout of an oil palm defense‐related gene to the pathogenic fungus Ganoderma boninense. Indonesian Journal of Biotechnology, 24(2), 101–105. https://doi.org/10.22146/ijbiotech.52170
Salin
IEEE
A. Budiani, I. B. Nugroho, D. A. Sari, I. Palupi, and R. A. Putranto, “CRISPR/Cas9‐mediated knockout of an oil palm defense‐related gene to the pathogenic fungus Ganoderma boninense,” Indonesian Journal of Biotechnology, vol. 24, no. 2, pp. 101–105, 2020, doi: 10.22146/ijbiotech.52170.
Salin
Dibuat dari metadata jurnal; periksa nama penulis sebelum dipakai.