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In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system

Indonesian Journal of Biotechnology · Vol. 25 No. 2 (2020) · hlm. 69–75

DOI
10.22146/ijbiotech.54703
ISSN
2089-2241, 0853-8654
Terbit
2020-11-10
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The aim of this work was the in vitro expression of the recombinant fusion (F) protein of Newcastle disease virus (NDV). The pBT7-N-His-Fusion-NDV expression plasmid which carries the recombinant F protein encoding gene from local Indonesian isolates, was prepared and transformed into E. coli BL21 (DE3). To detect bacterial colonies carrying the recombinant plasmid, a restriction endonuclease analysis was performed using the EcoRI restriction endonuclease. These results showed that the pBT-N-His-Fusion-NDV plasmid was successfully isolated with a size of 4.601 bp, and three recombinant plasmids carrying the gene coding for the recombinant F protein of NDV were obtained. Selected recombinant plasmids were then in vitro by using a cell-free protein expression system followed by visualization of the recombinant F protein on a 12% SDS-PAGE gel both by Coomassie Brilliant Blue staining and Western blotting. Recombinant F protein was successfully in vitro expressed by using a cell-free protein expression system as indicated by a specific single protein band with a molecular mass of 25.6 kDa.

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Rekan penulisOrganisasiArtikel bersama
Michael KannAfiliasi tidak diketahui7
Tri WibawaUniversitas Gadjah Mada4
Krisna Noli AndrianAfiliasi tidak diketahui4
Warih Pulung NugrahaniAfiliasi tidak diketahui3
Ulayatul KustiatiAfiliasi tidak diketahui3
Diana SavitriAfiliasi tidak diketahui2
Irhamna PutriAfiliasi tidak diketahui2
Medania PurwaningrumAfiliasi tidak diketahui2
Teguh BudipitojoAfiliasi tidak diketahui2
Dwi Liliek KusindartaAfiliasi tidak diketahui2
Hera NirwatiAfiliasi tidak diketahui2
S. SutaryoAfiliasi tidak diketahui2
Nenny Sri MulyaniAfiliasi tidak diketahui2
Titis WidowatiAfiliasi tidak diketahui2
Purnomo HadiAfiliasi tidak diketahui2
Devita Uzlah SibaraniAfiliasi tidak diketahui1
Sherly GusnawanAfiliasi tidak diketahui1
Lutfi MuzaqiAfiliasi tidak diketahui1
Dyah SukendraAfiliasi tidak diketahui1
Nungki Hapsari SuryaningtyasAfiliasi tidak diketahui1
Beni ErnawanAfiliasi tidak diketahui1
Wan Fatma ZuharahAfiliasi tidak diketahui1
Yunita Dyah Puspita SantikAfiliasi tidak diketahui1
Intan Tasya FarasyaAfiliasi tidak diketahui1
Najiha RamadhaniAfiliasi tidak diketahui1
Eldasari Myana Yulianingrum YulianingrumAfiliasi tidak diketahui1
Rinawati RinawatiAfiliasi tidak diketahui1
E SuharyantoAfiliasi tidak diketahui1
Muhammad AdnanAfiliasi tidak diketahui1
Matin NuhamunadaAfiliasi tidak diketahui1
Lisna HidayatiAfiliasi tidak diketahui1
Abdul Rahman SiregarAfiliasi tidak diketahui1
Shinta HartantoAfiliasi tidak diketahui1
Sofia MubarikaAfiliasi tidak diketahui1
Camelia HerdiniAfiliasi tidak diketahui1
Bambang HariwiyantoAfiliasi tidak diketahui1
Akira HosoyamaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system</dc:title>
	<dc:creator>Haryanto, Aris</dc:creator>
	<dc:creator>Wihadmadyatami, Hevi</dc:creator>
	<dc:creator>Wijayanti, Nastiti</dc:creator>
	<dc:subject xml:lang="en-US">cell-free protein expression; in vitro expression; Newcastle diseases virus (NDV); recombinant F protein</dc:subject>
	<dc:description xml:lang="en-US">The aim of this work was the in vitro expression of the recombinant fusion (F) protein of Newcastle disease virus (NDV).  The pBT7-N-His-Fusion-NDV expression plasmid which carries the recombinant F protein encoding gene from local Indonesian isolates, was prepared and transformed into E. coli BL21 (DE3). To detect bacterial colonies carrying the recombinant plasmid, a restriction endonuclease analysis was performed using the EcoRI restriction endonuclease. These results showed that the pBT-N-His-Fusion-NDV plasmid was successfully isolated with a size of 4.601 bp, and three recombinant plasmids carrying the gene coding for the recombinant F protein of NDV were obtained. Selected recombinant plasmids were then in vitro by using a cell-free protein expression system followed by visualization of the recombinant F protein on a 12% SDS-PAGE gel both by Coomassie Brilliant Blue staining and Western blotting. Recombinant F protein was successfully in vitro expressed by using a cell-free protein expression system as indicated by a specific single protein band with a molecular mass of 25.6 kDa.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">DRPM Kemenristekdikti</dc:contributor>
	<dc:date>2020-11-10</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/54703</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.54703</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 25, No 2 (2020); 69-75</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/54703/29742</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/54703/13184</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/54703/13185</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/54703/13186</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/54703/13187</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/54703/13188</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/54703/13482</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>