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Ethanolic extract of sappan wood (Caesalpinia sappan L.) inhibits MCF-7 and MCF-7/HER2 mammospheres' formation: an in vitro and bioinformatic study

Indonesian Journal of Biotechnology · Vol. 26 No. 3 (2021) · hlm. 133–141

DOI
10.22146/ijbiotech.63510
ISSN
2089-2241, 0853-8654
Terbit
2021-09-30
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

One of the mechanisms of cancer cell resistance toward chemotherapy is through cancer stem cells (CSCs), which are characterized by excessive activation of regulator proteins such as human epidermal receptor 2 (HER2). Sappan wood (Caesalpinia sappan L.) contains brazilin and brazilein that exhibit cytotoxic effects on several cancer cell lines. We aimed to explore the potency of the ethanolic extract of sappan (EES) in CSCs through bioinformatic analyses and by using a three-dimensional (3D) breast cancer stem cells (BCSCs) for in vitro assay with two different models (i.e., BCSCs and HER2-BCSCs) in order to identify the potential therapeutic targets of genes (PTTGs). Bioinformatic analyses identified PTTGs, which were further analyzed by gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, protein-protein interaction (PPI) networks, and hub protein selection. Mammospheres were cultured under conditioned media. The cytotoxic effects of EES were then measured by direct counting and based on the mammosphere-forming potential (MFP). Bioinformatic analysis disclosed PIK3CA and TP53 as PTTGs in BCSCs and HER2-BCSCs, respectively. In addition, the KEGG pathway analyses also demonstrated that PTTGs could regulate the ERBB pathway. EES thus demonstrated cytotoxicity and inhibited the formation of mammospheres. Collectively, EES exhibited excellent potential for further development as an inhibitor of cancer stem cells in breast cancer.

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  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Kementerian Kesehatan Republik Indonesia
  • Nara Institute of Science and Technology
  • Atma Jaya University Yogyakarta
  • Muhammadiyah University Purwokerto
  • Tanjungpura University
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Rekan penulisOrganisasiArtikel bersama
Riris Istighfari JenieUniversitas Gadjah Mada22
Ratna Asmah SusidartiUniversitas Gadjah Mada21
Muthi IkawatiUniversitas Gadjah Mada13
Rohmad Yudi UtomoUniversitas Gadjah Mada12
Dyaningtyas Dewi Pamungkas PutriUniversitas Gadjah Mada9
Faaza Aulia RahmanUniversitas Gadjah Mada6
Sari HaryantiKementerian Kesehatan Republik Indonesia6
Retno MurwantiUniversitas Gadjah Mada5
Desty Restia RahmawatiUniversitas Gadjah Mada5
Anindyajati AnindyajatiUniversitas Gadjah Mada5
Sri HandayaniIndonesian Institute of Sciences4
Larasati LarasatiUniversitas Gadjah Mada4
Lailatul QodriaUniversitas Gadjah Mada4
Gergorius Gena MaranUniversitas Gadjah Mada4
Beni LestariUniversitas Gadjah Mada4
Yurista GilangUniversitas Gadjah Mada4
Herwandhani PutriUniversitas Gadjah Mada4
Ummi Maryam ZulfinUniversitas Gadjah Mada3
Erlina RivantiUniversitas Gadjah Mada3
Sendy JunediAtma Jaya University Yogyakarta3
Sofa FaridaUniversitas Gadjah Mada3
Indri KusharyantiTanjungpura University3
Yuni Fajar EstiUniversitas Gadjah Mada3
Masashi KawaichiNara Institute of Science and Technology2
Nunuk Aries NurulitaMuhammadiyah University Purwokerto2
Suwijiyo PramonoUniversitas Gadjah Mada2
Zalinar UdinIndonesian Institute of Sciences2
Yogi ErtantoUniversitas Gadjah Mada2
Aulia Nur SeptianiUniversitas Gadjah Mada2
Asri Mega PutriUniversitas Gadjah Mada2
Hilyatul FadliyahUniversitas Gadjah Mada2
Yonika Arum LarasatiUniversitas Gadjah Mada2
Raditya Prima IstiajiUniversitas Gadjah Mada2
Ni Putu Linda LaksmianiUdayana University2
Astrid Ayu MarutiUniversitas Gadjah Mada2

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Ethanolic extract of sappan wood (Caesalpinia sappan L.) inhibits MCF-7 and MCF-7/HER2 mammospheres' formation: an in vitro and bioinformatic study</dc:title>
	<dc:creator>Novitasari, Dhania</dc:creator>
	<dc:creator>Muntafiah, Laeli</dc:creator>
	<dc:creator>Sari, Nur Fitra</dc:creator>
	<dc:creator>Meiyanto, Edy</dc:creator>
	<dc:creator>Hermawan, Adam</dc:creator>
	<dc:subject xml:lang="en-US">Bioinformatics; breast cancer stem cells; sappan wood (Caesalpinia sappan L.)</dc:subject>
	<dc:description xml:lang="en-US">One of the mechanisms of cancer cell resistance toward chemotherapy is through cancer stem cells (CSCs), which are characterized by excessive activation of regulator proteins such as human epidermal receptor 2 (HER2). Sappan wood (Caesalpinia sappan L.) contains brazilin and brazilein that exhibit cytotoxic effects on several cancer cell lines. We aimed to explore the potency of the ethanolic extract of sappan (EES) in CSCs through bioinformatic analyses and by using a three-dimensional (3D) breast cancer stem cells (BCSCs) for in vitro assay with two different models (i.e., BCSCs and HER2-BCSCs) in order to identify the potential therapeutic targets of genes (PTTGs). Bioinformatic analyses identified PTTGs, which were further analyzed by gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, protein-protein interaction (PPI) networks, and hub protein selection. Mammospheres were cultured under conditioned media. The cytotoxic effects of EES were then measured by direct counting and based on the mammosphere-forming potential (MFP). Bioinformatic analysis disclosed PIK3CA and TP53 as PTTGs in BCSCs and HER2-BCSCs, respectively. In addition, the KEGG pathway analyses also demonstrated that PTTGs could regulate the ERBB pathway. EES thus demonstrated cytotoxicity and inhibited the formation of mammospheres. Collectively, EES exhibited excellent potential for further development as an inhibitor of cancer stem cells in breast cancer.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Ministry of Research, Technology and Higher Education of the Republic of Indonesia for granting this study through Program Kreativitas Mahasiswa (PKM-PE) 2016.</dc:contributor>
	<dc:date>2021-09-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/63510</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.63510</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 26, No 3 (2021); 133-141</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/63510/32065</dc:relation>
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	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/63510/15485</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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