Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Performance of CO1 and ITS2 nested PCR in molecular identification of ordinary scabies (Sarcoptes scabiei var. hominis)

Indonesian Journal of Biotechnology · Vol. 26 No. 4 (2021) · hlm. 183–189

DOI
10.22146/ijbiotech.64472
ISSN
2089-2241, 0853-8654
Terbit
2021-12-31
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Scabies is a global disease with a high prevalence, causing morbidity and even mortality, especially in poor and developing countries. However, it is often misdiagnosed due to varied and unspecified lesions. The gold standard technique for diagnosis is a microscopic examination, which requires experienced experts in finding mites, mainly in ordinary scabies. CO1 and ITS2 genes have been widely used in molecular identification to detect Sarcoptes scabiei and its variants. This study aimed to determine and compare the sensitivity and specificity of CO1 and ITS2 S. scabiei genes to the microscopic examination of scabies skin scrapings. The skin scrapings of 52 subjects with scabies diagnosed by anamnesis, physical examination, and dermoscopic examination were examined under a microscope and analyzed by nested PCR. The diagnostic test result showed that the sensitivity of nested PCR of both CO1 and ITS2 genes to micro‐ scope examination was 100%. However, the specificity of both CO1 and ITS2 nested PCR was poor (24% and 0%). Hence, CO1 and ITS2 nested PCR could be more suitable for screening ordinary scabies in humans than the microscopic examination.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 117 tautan kepenulisan bersama · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Padjadjaran University
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Teguh KuncoroAfiliasi tidak diketahui2
Muhammad IlyasAfiliasi tidak diketahui2
Gunanti MahasriAfiliasi tidak diketahui2
Anord Charles NkubaAfiliasi tidak diketahui2
Adamu Ayubu MwendolwaAfiliasi tidak diketahui2
Mufasirin MufasirinAfiliasi tidak diketahui2
Jumina JuminaAfiliasi tidak diketahui2
Aprilita HajarAfiliasi tidak diketahui1
MaskuriAfiliasi tidak diketahui1
Abdul JalilAfiliasi tidak diketahui1
Soegiyanto SoegiyantoAfiliasi tidak diketahui1
Tri RustiadiAfiliasi tidak diketahui1
Bagas KurniantoAfiliasi tidak diketahui1
Aisyah Sausan FadhilaAfiliasi tidak diketahui1
Nungki MaulidiyanaAfiliasi tidak diketahui1
Achmad Figo Danendra RachmanAfiliasi tidak diketahui1
Alfaiz Alafi LuthfieAfiliasi tidak diketahui1
Fadhil Reza MahendraAfiliasi tidak diketahui1
LisdianaAfiliasi tidak diketahui1
Siti Harnina BintariAfiliasi tidak diketahui1
Rosyada ElvianiAfiliasi tidak diketahui1
Rico Januar SitorusAfiliasi tidak diketahui1
Aat SriatiPadjadjaran University1
Efri WidiantiAfiliasi tidak diketahui1
Dyah Ayu KurniawatiAfiliasi tidak diketahui1
Lucia Tri SuwantiAfiliasi tidak diketahui1
Setiawan KoesdartoAfiliasi tidak diketahui1
Endang SuprihatiAfiliasi tidak diketahui1
Arif PratiwiAfiliasi tidak diketahui1
Kusnoto KusnotoAfiliasi tidak diketahui1
Ayu Komang Ria Trie Dewi PinatihAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Performance of CO1 and ITS2 nested PCR in molecular identification of ordinary scabies (Sarcoptes scabiei var. hominis)</dc:title>
	<dc:creator>Prasasty, Gita Dwi</dc:creator>
	<dc:creator>Miftahurrizqiyah, Miftahurrizqiyah</dc:creator>
	<dc:creator>Anwar, Chairil</dc:creator>
	<dc:creator>Handayani, Dwi</dc:creator>
	<dc:creator>Dalilah, Dalilah</dc:creator>
	<dc:creator>Ghiffari, Ahmad</dc:creator>
	<dc:creator>Aryani, Inda Astri</dc:creator>
	<dc:creator>Lastuti, Nunuk Dyah Retno</dc:creator>
	<dc:creator>Berbudi, Afiat</dc:creator>
	<dc:subject xml:lang="en-US">sensitivity; CO1; ITS2; scabies</dc:subject>
	<dc:description xml:lang="en-US">Scabies is a global disease with a high prevalence, causing morbidity and even mortality, especially in poor and developing countries. However, it is often misdiagnosed due to varied and unspecified lesions. The gold standard technique for diagnosis is a microscopic examination, which requires experienced experts in finding mites, mainly in ordinary scabies. CO1 and ITS2 genes have been widely used in molecular identification to detect Sarcoptes scabiei and its variants. This study aimed to determine and compare the sensitivity and specificity of CO1 and ITS2 S. scabiei genes to the microscopic examination of scabies skin scrapings. The skin scrapings of 52 subjects with scabies diagnosed by anamnesis, physical examination, and dermoscopic examination were examined under a microscope and analyzed by nested PCR. The diagnostic test result showed that the sensitivity of nested PCR of both CO1 and ITS2 genes to micro‐ scope examination was 100%. However, the specificity of both CO1 and ITS2 nested PCR was poor (24% and 0%). Hence, CO1 and ITS2 nested PCR could be more suitable for screening ordinary scabies in humans than the microscopic examination.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Sriwijaya University</dc:contributor>
	<dc:date>2021-12-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/64472</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.64472</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 26, No 4 (2021); 183-189</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/64472/32895</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/64472/16033</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/64472/16034</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/64472/16035</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/64472/16036</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/64472/16037</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/64472/16038</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/64472/16039</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>