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Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Indonesian Journal of Biotechnology · Vol. 26 No. 4 (2021) · hlm. 206–213

DOI
10.22146/ijbiotech.65989
ISSN
2089-2241, 0853-8654
Terbit
2021-12-31
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Streptomyces is one of mercury‐resistant bacteria which can convert Hg2+ into nontoxic Hg0. This study aimed to identify mercury‐resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small‐scale gold mining (ASGM) area and clone merA gene to the cloning and expression vectors. Molecular identification was conducted using 16s rRNA gene with the maximum likelihood algorithms. Results revealed that the AS1 and AS2 strains were a group of Streptomyces ardesiacus and the BR28 strain was closed to Brevibacillus agri. The AS2 merA gene was cloned to pMD20 cloning vectors, pGEX‐5x‐1 and pET‐28c expression vectors. The transformation was successfully performed in BL21 and DH5α competent cells. The full length of the merA gene was confirmed to be 1,425 bp. This study is the first research on identifying mercury‐resistant Streptomyces and cloning the full‐length merA gene in Indonesia.

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  • Universitas Gadjah Mada
  • Universitas Muhammadiyah Pontianak
  • Muhammadiyah University of Yogyakarta
  • Jenderal Soedirman University
  • Universitas Islam Negeri Sultan Maulana Hasanuddin Banten
  • Nara Institute of Science and Technology
  • Universitas Halu Oleo
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Rekan penulisOrganisasiArtikel bersama
Ardaning NurilianiUniversitas Gadjah Mada4
Didik IndradewaUniversitas Gadjah Mada3
Widya AsmaraUniversitas Gadjah Mada3
Wayan Tunas ArtamaUniversitas Gadjah Mada3
Sri DarmawatiUniversitas Muhammadiyah Semarang3
Charlie Ester de FretesNational Research and Innovation Agency3
Rarastoeti PratiwiUniversitas Gadjah Mada3
Ari SunandarUniversitas Muhammadiyah Pontianak2
M. MurwantokoAfiliasi tidak diketahui2
Nur Arfa YantiUniversitas Halu Oleo2
Sebastian MarginoUniversitas Gadjah Mada2
Edy MeiyantoUniversitas Gadjah Mada2
Woro Anindito Sri TunjungUniversitas Gadjah Mada2
Radhiyah Mardhiyah HamidUniversitas Gadjah Mada2
Rifqi Zahroh JanatunaimUniversitas Gadjah Mada2
Tri Rini NuringtyasUniversitas Gadjah Mada2
Donny WidiantoUniversitas Gadjah Mada2
Alfino SebastianUniversitas Gadjah Mada2
Yuni SineUniversitas Gadjah Mada2
Taryono TaryonoUniversitas Gadjah Mada2
Ratna Dewi EskundariUniversitas Bangun Nusantara2
Yoshihiko YanoKobe University Hospital1
Yoni AstutiMuhammadiyah University of Yogyakarta1
Anisa Zuliana UtamiMuhammadiyah University of Yogyakarta1
Agus SuhartoMuhammadiyah University of Yogyakarta1
Resti AprianiUniversitas Muhammadiyah Pontianak1
Friti May SandiUniversitas Muhammadiyah Pontianak1
Mahwar QurbaniahUniversitas Muhammadiyah Pontianak1
Nurhayati NurhayatiUniversitas Muhammadiyah Pontianak1
Taufik Budi PramonoAfiliasi tidak diketahui1
Norman Arie PrayogoJenderal Soedirman University1
Asrul Sahri SiregarJenderal Soedirman University1
Purnama SukardiJenderal Soedirman University1
Bima MahendraUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene</dc:title>
	<dc:creator>Putri, Wahyu Aristyaning</dc:creator>
	<dc:creator>Rahayu, Hanum Mukti</dc:creator>
	<dc:creator>Khasanah, Anis Uswatun</dc:creator>
	<dc:creator>Sembiring, Langkah</dc:creator>
	<dc:creator>Kawaichi, Masashi</dc:creator>
	<dc:creator>Purwestri, Yekti Asih</dc:creator>
	<dc:subject xml:lang="en-US">artisanal gold mining, mercuric reductase, 16s rRNA, plasmid vector</dc:subject>
	<dc:description xml:lang="en-US">Streptomyces is one of mercury‐resistant bacteria which can convert Hg2+ into nontoxic Hg0 . This study aimed to identify mercury‐resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small‐scale gold mining (ASGM) area and clone merA gene to the cloning and expression vectors. Molecular identification was conducted using 16s rRNA gene with the maximum likelihood algorithms. Results revealed that the AS1 and AS2 strains were a group of Streptomyces ardesiacus and the BR28 strain was closed to Brevibacillus agri. The AS2 merA gene was cloned to pMD20 cloning vectors, pGEX‐5x‐1 and pET‐28c expression vectors. The transformation was successfully performed in BL21 and DH5α competent cells. The full length of the merA gene was confirmed to be 1,425 bp. This study is the first research on identifying mercury‐resistant Streptomyces and cloning the full‐length merA gene in Indonesia.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Universitas Gadjah Mada, Nara Institute of Science and Technology</dc:contributor>
	<dc:date>2021-12-31</dc:date>
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	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
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	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/65989</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.65989</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 26, No 4 (2021); 206-213</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
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	<dc:rights xml:lang="en-US">Copyright (c) 2021 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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