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Effect of galangal essential oils on rumen microbial population and biodiversity on in vitro rumen fermentation

Indonesian Journal of Biotechnology · Vol. 27 No. 4 (2022) · hlm. 195–202

DOI
10.22146/ijbiotech.66680
ISSN
2089-2241, 0853-8654
Terbit
2022-12-30
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The study aimed to evaluate the effect of administering galangal essential oil (EO) on the abundance of rumen bacteria using the 16s rRNA method. The treatments included a control (no EO addition), galangal EO (30, 60, 120 µL), and cineole (5 µL). The treatments were assessed using a 48‐hour in vitro batch culture of rumen fluid containing a 60:40 ratio of forage to concentrate. For amplification of the prokaryotes (bacteria and archaea) in region V4, 16s rRNA primer 5’GTGCCAGCMGCCGCGTAA, GGACTACHVGGGTWTCTAAT3’ was employed. The data for rumen microbial abundance were analysed descriptively, while the data for rumen microbial diversity were obtained from the report on the Next Generation Sequencing Method. The microbial composition of each sample was tested for operational taxonomic units (OTUs) with a 97% identity rate on a valid label. The 16S rRNA gene sequencing yielded a total of 3,977 OTUs. Adding galangal and cineole EOs resulted in the same variation of the Shannon index. The population index (chao1 index) was highest when 60 µL of galangal EO was added, compared to 30 and 120 µL of galangal EO and cineole. In addition, providing 60 µL of galangal EO decreased the abundance of Prevotella ruminicola compared to the control and cineole doses. The addition of galangal EO also led to a decline in the number of Methanobacteriales. The population of the fibre‐degrading bacteria group (Ruminococcus albus and Ruminococcus flavefaciens) was higher in a dose of galangal EO than the control and cineole. Therefore, it can be concluded that the effective dose of galangal EO, i.e. 60 µL/300 mg (DM feed) in vitro, can reduce the abundance of Prevotella bacteria and methanogens.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Politeknik Pembangunan Pertanian Malang
  • Universitas Muhammadiyah Purworejo
  • IPB University
  • Nagoya University
  • Indonesian Institute of Sciences
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Rekan penulisOrganisasiArtikel bersama
Muhamad Nur CahyantoUniversitas Gadjah Mada2
Ali WibowoUniversitas Gadjah Mada2
Setiyono SetiyonoUniversitas Gadjah Mada1
Roisu Eny MudawarochUniversitas Muhammadiyah Purworejo1
Edi SuryantoUniversitas Gadjah Mada1
Widodo WidodoIPB University1
Asih KurniawatiUniversitas Gadjah Mada1
Wayan Tunas ArtamaUniversitas Gadjah Mada1
Pradita Iustitia SitaresmiNational Research and Innovation Agency1
Dio Fico Felsidan DiatmonoUniversitas Gadjah Mada1
Fransisca Gani PadmawatiUniversitas Gadjah Mada1
Sungkono SungkonoUniversitas Gadjah Mada1
Andrian SyahUniversitas Gadjah Mada1
Herdis HerdisNational Research and Innovation Agency1
Satoshi OhkuraNagoya University1
Diah Tri WidayatiUniversitas Gadjah Mada1
Ema DamayantiIndonesian Institute of Sciences1
Achmad DinotoNational Research and Innovation Agency1
Ahimsa Kandi SaririUniversitas Bangun Nusantara1
Kustantinah KustantinahUniversitas Gadjah Mada1
Zaenal BachruddinUniversitas Gadjah Mada1
Cahya Setya UtamaDiponegoro University1
Zuprizal ZuprizalUniversitas Gadjah Mada1
Wihandoyo WihandoyoUniversitas Gadjah Mada1
Nafiatul UmamiUniversitas Gadjah Mada1
Adib Norma RespatiPoliteknik Negeri Jember1
Agung IrawanSebelas Maret University1
Lukita PurnamayantiUniversitas Gadjah Mada1
Jamhari JamhariUniversitas Gadjah Mada1
AgussalimUniversitas Gadjah Mada1
Ida Ketut MudhitaUniversitas Antakusuma1
Ryan Aryadin PutraUniversity of Mataram1
M. M. RahmanUniversiti Malaysia Kelantan1
N. UmamiUniversitas Gadjah Mada1
Siti ZubaidahState University of Malang1
Bambang AriyadiUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Effect of galangal essential oils on rumen microbial population and biodiversity on in vitro rumen fermentation</dc:title>
	<dc:creator>Daning, Dewi Ratih Ayu</dc:creator>
	<dc:creator>Widyobroto, Budi Prasetyo</dc:creator>
	<dc:creator>Yusiati, Lies Mira</dc:creator>
	<dc:creator>Hanim, Chusnul</dc:creator>
	<dc:subject xml:lang="en-US">galangal essential oil; microbial diversity; rumen fermentation; 16sRNA</dc:subject>
	<dc:description xml:lang="en-US">The study aimed to evaluate the effect of administering galangal essential oil (EO) on the abundance of rumen bacteria using the 16s rRNA method. The treatments included a control (no EO addition), galangal EO (30, 60, 120 µL), and cineole (5 µL). The treatments were assessed using a 48‐hour in vitro batch culture of rumen fluid containing a 60:40 ratio of forage to concentrate. For amplification of the prokaryotes (bacteria and archaea) in region V4, 16s rRNA primer 5’GTGCCAGCMGCCGCGTAA, GGACTACHVGGGTWTCTAAT3’ was employed. The data for rumen microbial abundance were analysed descriptively, while the data for rumen microbial diversity were obtained from the report on the Next Generation Sequencing Method. The microbial composition of each sample was tested for operational taxonomic units (OTUs) with a 97% identity rate on a valid label. The 16S rRNA gene sequencing yielded a total of 3,977 OTUs. Adding galangal and cineole EOs resulted in the same variation of the Shannon index. The population index (chao1 index) was highest when 60 µL of galangal EO was added, compared to 30 and 120 µL of galangal EO and cineole. In addition, providing 60 µL of galangal EO decreased the abundance of Prevotella ruminicola compared to the control and cineole doses. The addition of galangal EO also led to a decline in the number of Methanobacteriales. The population of the fibre‐degrading bacteria group (Ruminococcus albus and Ruminococcus flavefaciens) was higher in a dose of galangal EO than the control and cineole. Therefore, it can be concluded that the effective dose of galangal EO, i.e. 60 µL/300 mg (DM feed) in vitro, can reduce the abundance of Prevotella bacteria and methanogens.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Doctoral Scholarship, Ministry of Agriculture of the Republic of Indonesia</dc:contributor>
	<dc:date>2022-12-30</dc:date>
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	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/66680</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.66680</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 27, No 4 (2022); 195-202</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/66680/35505</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/66680/17666</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/66680/17667</dc:relation>
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	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/66680/17672</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 The Author(s)</dc:rights>
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