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Anti‐diabetic effect of andrographolide from Sambiloto herbs (Andrographis paniculata (Burm.f.) Nees) through the expression of PPARγ and GLUT‐4 in adipocytes

Indonesian Journal of Biotechnology · Vol. 27 No. 4 (2022) · hlm. 203–211

DOI
10.22146/ijbiotech.68800
ISSN
2089-2241, 0853-8654
Terbit
2022-12-30
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Andrographolide has been shown to have a pharmacological effect as an antidiabetic. Nevertheless, the comprehensive mechanism of action has yet to be determined. Andrographolide is a primary component of the sambiloto herb (Andrographis paniculata (Burm.f.) Nees), in which a simple isolation process can obtain high yields. This study aimed to explain the anti‐diabetic effect of andrographolide compared to pioglitazone (a positive control) on glucose uptake by measuring the expression levels of peroxisome proliferator‐activated receptor gamma (PPARγ) and glucose transporter type 4 (GLUT‐4) genes in 3T3‐LI mouse adipocytes as an in vitro model. The differentiation of mature adipocytes from 3T3‐L1 fibroblasts was induced with 3‐isobutyl‐1‐methylxanthine, dexamethasone, and insulin. Andrographolide was provided through direct isolation from A. paniculata herbs. The gene expression was detected using the reverse transcription‐polymerase chain reaction (RT‐PCR). Pioglitazone and andrographolide significantly increased glucose uptake capability. Andrographolide was able to increase the mRNA levels of PPARγ and GLUT‐4 compared to pioglitazone with the best concentration at 5.6 µM. In conclusion, andrographolide can improve glucose uptake by increasing mRNA levels of PPARγ and GLUT‐4 that encodes protein, which are key factors for glucose homeostasis. Therefore, this finding further establishes the potency of andrographolide from A. paniculata as an antidiabetic.

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  • Universitas Gadjah Mada
  • Universitas Ahmad Dahlan
  • Bandung Institute of Technology
  • PT Dexa Medica (Indonesia)
  • IPB University
  • Atma Jaya Catholic University of Indonesia
  • Kementerian Kesehatan Republik Indonesia
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Rekan penulisOrganisasiArtikel bersama
Edy MeiyantoUniversitas Gadjah Mada3
Sari HaryantiKementerian Kesehatan Republik Indonesia2
Retno MurwantiUniversitas Gadjah Mada2
Anindyajati AnindyajatiUniversitas Gadjah Mada2
Maggy Thenawidjaja SuhartonoIPB University1
Wenny Silvia L. Br. SinagaIPB University1
Wangsa Tirta IsmayaAfiliasi tidak diketahui1
Debbie S. RetroningrumBandung Institute of Technology1
DEBBIE SOEFIE RETNONINGRUMBandung Institute of Technology1
Anita ArtariniBandung Institute of Technology1
Armini SyamsidiBandung Institute of Technology1
S. SardjimanAfiliasi tidak diketahui1
Kazutaka MaeyamaAfiliasi tidak diketahui1
Nadia WidjajaPT Dexa Medica (Indonesia)1
Stefeny Theresia SimatupangPT Dexa Medica (Indonesia)1
Santi TanPT Dexa Medica (Indonesia)1
Ika NurzijahUniversitas Gadjah Mada1
Dita Brenna SeptheaUniversitas Gadjah Mada1
Andita Pra DarmaUniversitas Gadjah Mada1
Herwandhani PutriUniversitas Gadjah Mada1
Gagas Pradani Nur IlmawatiUniversitas Gadjah Mada1
Fatma NuraisyahUniversitas Ahmad Dahlan1
Pramudita BudiastutiUniversitas Ahmad Dahlan1
Adi PermadiUniversitas Ahmad Dahlan1
Barry Nur SetyantoUniversitas Ahmad Dahlan1
Rachmadian WulandanaSUNY New Paltz1
Arief SyamsuddinUniversitas Ahmad Dahlan1
Muhammad Kaisar Sutomo RamadhanUniversitas Ahmad Dahlan1
Andi Rifki RosandyBandung Institute of Technology1
Sugeng RiyantoUniversitas Gadjah Mada1
Puguh NoviarsitoMuhammadiyah University of Yogyakarta1
Prajona MarbunAfiliasi tidak diketahui1
Endang LukitaningsihUniversitas Gadjah Mada1
Annik MegawatiUniversitas Gadjah Mada1
Arief NurrochmadUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Anti‐diabetic effect of andrographolide from Sambiloto herbs (Andrographis paniculata (Burm.f.) Nees) through the expression of PPARγ and GLUT‐4 in adipocytes</dc:title>
	<dc:creator>Astuti, Novia Tri</dc:creator>
	<dc:creator>Novitasari, Putri Rachma</dc:creator>
	<dc:creator>Tjandrawinata, Raymond</dc:creator>
	<dc:creator>Nugroho, Agung Endro</dc:creator>
	<dc:creator>Pramono, Suwijiyo</dc:creator>
	<dc:subject xml:lang="en-US">3T3‐L1 adipocytes; andrographolide; glucose uptake; PPARγ; GLUT‐4</dc:subject>
	<dc:description xml:lang="en-US">Andrographolide has been shown to have a pharmacological effect as an antidiabetic. Nevertheless, the comprehensive mechanism of action has yet to be determined. Andrographolide is a primary component of the sambiloto herb (Andrographis paniculata (Burm.f.) Nees), in which a simple isolation process can obtain high yields. This study aimed to explain the anti‐diabetic effect of andrographolide compared to pioglitazone (a positive control) on glucose uptake by measuring the expression levels of peroxisome proliferator‐activated receptor gamma (PPARγ) and glucose transporter type 4 (GLUT‐4) genes in 3T3‐LI mouse adipocytes as an in vitro model. The differentiation of mature adipocytes from 3T3‐L1 fibroblasts was induced with 3‐isobutyl‐1‐methylxanthine, dexamethasone, and insulin. Andrographolide was provided through direct isolation from A. paniculata herbs. The gene expression was detected using the reverse transcription‐polymerase chain reaction (RT‐PCR). Pioglitazone and andrographolide significantly increased glucose uptake capability. Andrographolide was able to increase the mRNA levels of PPARγ and GLUT‐4 compared to pioglitazone with the best concentration at 5.6 µM. In conclusion, andrographolide can improve glucose uptake by increasing mRNA levels of PPARγ and GLUT‐4 that encodes protein, which are key factors for glucose homeostasis. Therefore, this finding further establishes the potency of andrographolide from A. paniculata as an antidiabetic.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Dexa Laboratories of Biomolecular Sciences (DLBS)</dc:contributor>
	<dc:contributor xml:lang="en-US">UGM Research Directorate through the 2019 Research Assistance Program Grant</dc:contributor>
	<dc:date>2022-12-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/68800</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.68800</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 27, No 4 (2022); 203-211</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
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	<dc:rights xml:lang="en-US">Copyright (c) 2022 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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