Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Expression of cytokinin responsive and ethylene biosynthesis genes in rice callus with different regeneration rates

Indonesian Journal of Biotechnology · Vol. 27 No. 2 (2022) · hlm. 65–72

DOI
10.22146/ijbiotech.70264
ISSN
2089-2241, 0853-8654
Terbit
2022-06-29
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

This study aimed to investigate the correlation between callus regeneration rate and the expression of several genes responsible for cytokinin response and ethylene biosynthesis in the Ciherang, Mentik Wangi Susu, Hwayoung and Tarabas rice varieties. The callus regeneration rate of each rice variety was in vitro tested using N6 media, while the gene expression during the callus regeneration stages was examined using quantitative real‐time PCR (qRT‐PCR). Our results showed that the callus of Ciherang and Mentik Wangi Susu showed earlier green spot formation that then turned brown at a later stage, resulting in a low regeneration rate. While Hwayoung and Tarabas showed late green spot formation, high shoot regeneration was observed in both calluses. Gene expression analysis of regeneration media‐grown calluses showed that two cytokinin‐responsive genes, OsRR2 and OsRR6, were highly expressed in the Ciherang and Hwayoung callus, respectively. We also observed that ethylene biosynthesis genes such as OsACS1 and OsACO1 were highly expressed in the Mentik Wangi Susu and Hwayoung callus, respectively. Moreover, the expression of OsBBM1 was high in Hwayoung and Tarabas. Thus, the positive correlation between the expression of cytokinin‐responsive and ethylene biosynthesis genes with somatic embryogenesis activity likely depends on the induction level of OsBBM1.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 116 tautan kepenulisan bersama · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Jember
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Didik Pudji RestantoAfiliasi tidak diketahui3
HARDIAN SUSILO ADDYAfiliasi tidak diketahui3
Tri HandoyoAfiliasi tidak diketahui2
Yusuf Dary AhnafAfiliasi tidak diketahui2
IryonoAfiliasi tidak diketahui2
Ali WafaAfiliasi tidak diketahui2
Ahmad Ilham TanzilAfiliasi tidak diketahui2
Dinda Mala MaulidaAfiliasi tidak diketahui2
Intan Ria NelianaAfiliasi tidak diketahui2
Cassidy MagnonAfiliasi tidak diketahui2
Sholeh AviviUniversitas Jember2
Galang Rizki RamadhanAfiliasi tidak diketahui2
Siti Nurul AfidahAfiliasi tidak diketahui2
Inyana Dwi AgustienAfiliasi tidak diketahui2
Popy Hartatie HardjoAfiliasi tidak diketahui1
Mohammad Candra PrayogaAfiliasi tidak diketahui1
Sigit SoeparjonoAfiliasi tidak diketahui1
Numalasari NumalasariAfiliasi tidak diketahui1
Falin Fatimatus ShahroAfiliasi tidak diketahui1
Fakhriyah Nur KhalilahAfiliasi tidak diketahui1
Hayati Aulia MaharaniAfiliasi tidak diketahui1
Kyung-Min KimAfiliasi tidak diketahui1
Intan Hadiatun MaghfirahAfiliasi tidak diketahui1
Ummi SholikhahAfiliasi tidak diketahui1
Tri Wahyu SaputraAfiliasi tidak diketahui1
Mohammad Nur KhozinAfiliasi tidak diketahui1
Mohammad UbaidillahAfiliasi tidak diketahui1
Nabila Nur Aisyah Al AyyubiAfiliasi tidak diketahui1
Rendryana Aulia Nur KhofifaAfiliasi tidak diketahui1
Slameto SlametoAfiliasi tidak diketahui1
Nur BasukiAfiliasi tidak diketahui1
Liliek SulistyowatiAfiliasi tidak diketahui1
Mohammad Nur KhozinAfiliasi tidak diketahui1
Weny Nailul HidayatiAfiliasi tidak diketahui1
Retnosari ApriastiAfiliasi tidak diketahui1
Nur Elia NadhiraAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Expression of cytokinin responsive and ethylene biosynthesis genes in rice callus with different regeneration rates</dc:title>
	<dc:creator>Husna, Syafira Fatihatul</dc:creator>
	<dc:creator>Dewanti, Parawita</dc:creator>
	<dc:creator>Sugiharto, Bambang</dc:creator>
	<dc:creator>Fanata, Wahyu Indra Duwi</dc:creator>
	<dc:subject xml:lang="en-US">rice callus; regeneration rates; ethylene biosynthesis; cytokinin response</dc:subject>
	<dc:description xml:lang="en-US">This study aimed to investigate the correlation between callus regeneration rate and the expression of several genes responsible for cytokinin response and ethylene biosynthesis in the Ciherang, Mentik Wangi Susu, Hwayoung and Tarabas rice varieties. The callus regeneration rate of each rice variety was in vitro tested using N6 media, while the gene expression during the callus regeneration stages was examined using quantitative real‐time PCR (qRT‐PCR). Our results showed that the callus of Ciherang and Mentik Wangi Susu showed earlier green spot formation that then turned brown at a later stage, resulting in a low regeneration rate. While Hwayoung and Tarabas showed late green spot formation, high shoot regeneration was observed in both calluses. Gene expression analysis of regeneration media‐grown calluses showed that two cytokinin‐responsive genes, OsRR2 and OsRR6, were highly expressed in the Ciherang and Hwayoung callus, respectively. We also observed that ethylene biosynthesis genes such as OsACS1 and OsACO1 were highly expressed in the Mentik Wangi Susu and Hwayoung callus, respectively. Moreover, the expression of OsBBM1 was high in Hwayoung and Tarabas. Thus, the positive correlation between the expression of cytokinin‐responsive and ethylene biosynthesis genes with somatic embryogenesis activity likely depends on the induction level of OsBBM1.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2022-06-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/70264</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.70264</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 27, No 2 (2022); 65-72</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/70264/34056</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/70264/18982</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/70264/18984</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/70264/18985</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/70264/18986</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/70264/18987</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/70264/18991</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>