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Antimicrobial compounds from intracellular and extracellular secondary metabolites of Actinobacteria InaCC A759

Indonesian Journal of Biotechnology · Vol. 29 No. 4 (2024) · hlm. 221–230

DOI
10.22146/ijbiotech.82376
ISSN
2089-2241, 0853-8654
Terbit
2024-12-31
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The World Health Organization (WHO) has determined a list of pathogens that require the development of new antimicrobials due to resistance problems; these include Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. In addition, Mycobacterium smegmatis has been used for antimycobacterial discovery to address the increasing burden of tuberculosis. In this study, optimization of antimicrobial activity, secondary metabolite profiling, and strain identification was conducted on Actinobacteria InaCC A759. Intracellular and extracellular extracts of Actinobacteria InaCC A759 were found to have different antimicrobial activities. The minimum inhibitory concentration (MIC) values of the extract to inhibit the growth of M. smegmatis, E. coli, and P. aeruginosa were 50, 25, and 100 µg/mL (intracellular), and 25, 25, and 100 µg/mL (extracellular), respectively. However, neither extract was able to inhibit the growth of S. aureus. Metabolite profiling using High resolution‐mass spectrometry (HR‐MS) resulted in differences in the major compound between the two extracts of Actinobacteria InaCC A759, namely n‐acetyltyramine (C10H13NO2/179.0945) (24.24%) (intracellular) and palmitic acid (C16H32O2/273.27034) (86.92%) (extracellular). Based on molecular analysis of the 16S rRNA gene, Actinobacteria InaCC A759 is identical to the Streptomyces olivaceus strain FoRh46. The antimicrobial activity and secondary metabolites profiles of Streptomyces olivaceus InaCC A759 have not been previously reported.

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  • Universitas Muhammadiyah Semarang
  • Universitas Gadjah Mada
  • Tadulako University
  • Universitas Jambi
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Rekan penulisOrganisasiArtikel bersama
Nastiti WijayantiAfiliasi tidak diketahui3
Jaka WidadaUniversitas Gadjah Mada2
Hisam AhyaniAfiliasi tidak diketahui2
Haris Maiza PutraAfiliasi tidak diketahui2
Tri Ratna SulistiyaniAfiliasi tidak diketahui2
Siti MeliahAfiliasi tidak diketahui2
Hera NirwatiAfiliasi tidak diketahui2
Farida J. RachmawatyAfiliasi tidak diketahui2
Marsetyawan H.N.E. SoesatyoAfiliasi tidak diketahui2
Danny Syamsu WAfiliasi tidak diketahui1
Andi Idhan MuchsinAfiliasi tidak diketahui1
Diani MentariAfiliasi tidak diketahui1
Nastiti WijayantiUniversitas Gadjah Mada1
Dwi Agus KurniawanUniversitas Jambi1
Devita DiatriUniversitas Muhammadiyah Semarang1
Lourensya Berta JoharlinaUniversitas Muhammadiyah Semarang1
Alif Adlan ZulizarUniversitas Muhammadiyah Semarang1
Siti KomariyahAfiliasi tidak diketahui1
Lilis YuliatiAfiliasi tidak diketahui1
Nely SupeniAfiliasi tidak diketahui1
Ahmad Mahfudzi MafrudloAfiliasi tidak diketahui1
Zuhratul Aini MansurAfiliasi tidak diketahui1
Yadi JanwariAfiliasi tidak diketahui1
Muharir MuharirAfiliasi tidak diketahui1
Encep Taufik RahmanAfiliasi tidak diketahui1
Iwan MulyanaAfiliasi tidak diketahui1
Dewi SadiahAfiliasi tidak diketahui1
Doddy Afandi FirdausAfiliasi tidak diketahui1
Arwansyah bin KirinAfiliasi tidak diketahui1
Zakiyyu MuhammadAfiliasi tidak diketahui1
Shanti RatnakomalaAfiliasi tidak diketahui1
Nita Rosalinda PrayitnoAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Antimicrobial compounds from intracellular and extracellular secondary metabolites of Actinobacteria InaCC A759</dc:title>
	<dc:creator>Rakhmawatie, Maya Dian</dc:creator>
	<dc:creator>Mustofa, Mustofa</dc:creator>
	<dc:creator>Lisdiyanti, Puspita</dc:creator>
	<dc:creator>Wibawa, Tri</dc:creator>
	<dc:creator>Ratnaningrum, Kanti</dc:creator>
	<dc:creator>Umam, Muhammad Mucharom Chairul</dc:creator>
	<dc:creator>Alfi, Muhammad Hasan</dc:creator>
	<dc:creator>Chariri, Listya</dc:creator>
	<dc:subject xml:lang="en-US">Actinobacteria; Antimicrobial agents; Metabolite profiling; N‐acetyltyramine; Palmitic acid</dc:subject>
	<dc:description xml:lang="en-US">The World Health Organization (WHO) has determined a list of pathogens that require the development of new antimicrobials due to resistance problems; these include Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. In addition, Mycobacterium smegmatis has been used for antimycobacterial discovery to address the increasing burden of tuberculosis. In this study, optimization of antimicrobial activity, secondary metabolite profiling, and strain identification was conducted on Actinobacteria InaCC A759. Intracellular and extracellular extracts of Actinobacteria InaCC A759 were found to have different antimicrobial activities. The minimum inhibitory concentration (MIC) values of the extract to inhibit the growth of M. smegmatis, E. coli, and P. aeruginosa were 50, 25, and 100 µg/mL (intracellular), and 25, 25, and 100 µg/mL (extracellular), respectively. However, neither extract was able to inhibit the growth of S. aureus. Metabolite profiling using High resolution‐mass spectrometry (HR‐MS) resulted in differences in the major compound between the two extracts of Actinobacteria InaCC A759, namely n‐acetyltyramine (C10H13NO2/179.0945) (24.24%) (intracellular) and palmitic acid (C16H32O2/273.27034) (86.92%) (extracellular). Based on molecular analysis of the 16S rRNA gene, Actinobacteria InaCC A759 is identical to the Streptomyces olivaceus strain FoRh46. The antimicrobial activity and secondary metabolites profiles of Streptomyces olivaceus InaCC A759 have not been previously reported.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2024-12-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/82376</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.82376</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 29, No 4 (2024); 221-230</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/82376/40744</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82376/25484</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82376/25485</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82376/25486</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82376/25487</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82376/25488</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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