Melola Discovery

← Melola Discovery

Akses terbuka Jurnal SINTA 1

Genetic evaluation of F2 and F3 interspecific hybrids of mung bean (Vigna radiata L. Wilczek) using retrotransposon‐based insertion polymorphism and sequence‐related amplified polymorphism markers

Indonesian Journal of Biotechnology · Vol. 28 No. 3 (2023) · hlm. 143–152

DOI
10.22146/ijbiotech.82760
ISSN
2089-2241, 0853-8654
Terbit
2023-09-30
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Mung bean (Vigna radiata L. Wilczek) is a self‐pollinating and indispensable pulse crop in Indonesia. While low yield productivity is a major concern, genetic improvement is possible through interspecific hybridization. However, interspecific hybridization is relatively infrequent and produces low recombination exchanges, significantly limiting crop breeding efficiency. Thus, a comprehensive study is needed of the selection and genetic diversity evaluation of progenies in advanced generations derived from interspecific hybridization using a specific molecular marker. This study aims to confirm the heterozygosity in the F2 population and assess the genetic diversity in F3 mung bean populations resulting from interspecific hybridization between the mung bean and common bean. We designed the retrotransposon‐based insertion polymorphism (RBIP) marker by identifying the syntenic regions in the flanking sequences of retrotransposon insertion in common bean and mung bean. The RBIP marker can be applied to distinguish the heterozygote progenies from the homozygote progenies. Six combinations of sequence‐related amplified polymorphism (SRAP) primers were used in the genotyping of F3 mung bean progenies. The SRAP marker showed a high degree of polymorphism of up to 100%, while high genetic variation was observed within the population (71%) of mung bean progenies. The F3.4 population had the greatest number of genotypes and displayed the highest number of effective alleles, private alleles, and percentage of polymorphic loci, suggesting the existence of high genetic diversity within this population. These genetic diversity data are exceptionally critical for future genetic research since it has potentially high yield production. The genomic and marker‐assisted selection studies will support the major goals of the mung bean breeding program.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 87 tautan kepenulisan bersama · 43 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Gadjah Mada
  • Universitas Syiah Kuala
  • Politeknik Pertanian Negeri Pangkajene Kepulauan
  • Hasanuddin University
  • State University of Semarang
  • University of Malaya
  • Indonesian Coffee and Cocoa Research Institute
  • Organisasi lain
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Endang SemiartiUniversitas Gadjah Mada11
Sukarti MoeljopawiroUniversitas Gadjah Mada5
Ari WibowoIndonesian Coffee and Cocoa Research Institute4
Ari IndriantoUniversitas Gadjah Mada3
Nintya SetiariDiponegoro University2
Muhammad Dylan LawrieUniversitas Gadjah Mada2
Eka Tarwaca Susila PutraUniversitas Gadjah Mada2
Exsyupransia MursyantiUniversitas Gadjah Mada2
Wahyudin WahyudinTadulako University1
Ita FitriatiSTKIP Taman Siswa Bima1
Anisa AnisaHasanuddin University1
Raupong RaupongHasanuddin University1
Mauli KasmiPoliteknik Pertanian Negeri Pangkajene Kepulauan1
Muhammad RidwanUniversitas Jambi1
Seniorita SenioritaPoliteknik Pertanian Negeri Pangkajene Kepulauan1
Andi Baso Adil NatsirPoliteknik Pertanian Negeri Pangkajene Kepulauan1
Tajuddin TajuddinUniversitas Halu Oleo1
Imam ShofwanState University of Semarang1
Amin YusufState University of Semarang1
Syamsul BahriUniversitas Syiah Kuala1
Faisal A. RaniUniversitas Syiah Kuala1
Sufyan SufyanUniversitas Syiah Kuala1
Suriyani SuriyaniUniversitas Islam Negeri Alauddin Makassar1
Prima Denny SentiaUniversitas Syiah Kuala1
Rizki Agam SyahputraUniversitas Teuku Umar1
Sarika ZuhriUniversitas Syiah Kuala1
Friesca ErwanSzéchenyi István University1
Zikra NopritaUniversitas Syiah Kuala1
Asadi AsadiAfiliasi tidak diketahui1
Sahiral YakubAfiliasi tidak diketahui1
B. SALLEH B. SALLEHAfiliasi tidak diketahui1
L. JULIAAfiliasi tidak diketahui1
A. SAFINATUniversiti Sains Malaysia1
Triono Bagus SaputroInstitut Teknologi Sepuluh Nopember1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Genetic evaluation of F2 and F3 interspecific hybrids of mung bean (Vigna radiata L. Wilczek) using retrotransposon‐based insertion polymorphism and sequence‐related amplified polymorphism markers</dc:title>
	<dc:creator>Fatmawati, Yeni</dc:creator>
	<dc:creator>Ilyas, Ilyas</dc:creator>
	<dc:creator>Setiawan, Agus Budi</dc:creator>
	<dc:creator>Purwantoro, Aziz</dc:creator>
	<dc:creator>Respatie, Dyah Weny</dc:creator>
	<dc:creator>Teo, Chee How</dc:creator>
	<dc:subject xml:lang="en-US">Genetic differentiation; Heterozygosity; Intergeneric hybrids; Mung bean breeding; RBIP marker</dc:subject>
	<dc:description xml:lang="en-US">Mung bean (Vigna radiata L. Wilczek) is a self‐pollinating and indispensable pulse crop in Indonesia. While low yield productivity is a major concern, genetic improvement is possible through interspecific hybridization. However, interspecific hybridization is relatively infrequent and produces low recombination exchanges, significantly limiting crop breeding efficiency. Thus, a comprehensive study is needed of the selection and genetic diversity evaluation of progenies in advanced generations derived from interspecific hybridization using a specific molecular marker. This study aims to confirm the heterozygosity in the F2 population and assess the genetic diversity in F3 mung bean populations resulting from interspecific hybridization between the mung bean and common bean. We designed the retrotransposon‐based insertion polymorphism (RBIP) marker by identifying the syntenic regions in the flanking sequences of retrotransposon insertion in common bean and mung bean. The RBIP marker can be applied to distinguish the heterozygote progenies from the homozygote progenies. Six combinations of sequence‐related amplified polymorphism (SRAP) primers were used in the genotyping of F3 mung bean progenies. The SRAP marker showed a high degree of polymorphism of up to 100%, while high genetic variation was observed within the population (71%) of mung bean progenies. The F3.4 population had the greatest number of genotypes and displayed the highest number of effective alleles, private alleles, and percentage of polymorphic loci, suggesting the existence of high genetic diversity within this population. These genetic diversity data are exceptionally critical for future genetic research since it has potentially high yield production. The genomic and marker‐assisted selection studies will support the major goals of the mung bean breeding program. </dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2023-09-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/82760</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.82760</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 28, No 3 (2023); 143-152</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/82760/37457</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82760/25704</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82760/25705</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82760/25706</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82760/25707</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82760/25708</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/82760/25709</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>