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Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device

Indonesian Journal of Biotechnology · Vol. 29 No. 1 (2024) · hlm. 33–39

DOI
10.22146/ijbiotech.83645
ISSN
2089-2241, 0853-8654
Terbit
2024-03-30
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Detecting Ganoderma boninense in Indonesia is crucial for effectively controlling and mitigating the spread of basal stem disease in oil palm fields. While there is ongoing development of tolerant plants, no such plant has been successfully created yet. Consequently, researchers are actively studying detection methods for Ganoderma boninense. One established and highly accurate approach is the use of polymerase chain reaction (PCR) techniques for molecular detection. However, this method requires time‐consuming sample preparation, which can pose challenges in plantation settings. To address this problem, a portable lab‐on‐chip device has been introduced. This technology enables easy and automatic DNA retrieval from liquid samples by absorbing lysed DNA using magnetic beads. An efficient mechanism for manipulating the magnetic bead within the semiconductor has been successfully implemented. The extraction process typically takes around 15 minutes using a modified methodology on the chip device approach. The chip facilitates the retrieval of two samples with a capacity of 120 µL for each sample. The PCR method was utilized to validate the equivalence of the lab‐on‐chip device extraction to the standard extraction method. This represents a promising alternative for expedited and simplified detection of Ganoderma boninense in field conditions.

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  • National Research and Innovation Agency
  • Indonesian Institute of Sciences
  • University of Indonesia
  • Universiti of Malaysia Sabah
  • Bandung Institute of Technology
  • Institut Teknologi Sepuluh Nopember
  • Hallym University
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Rekan penulisOrganisasiArtikel bersama
M. N. A. UdaUniversiti of Malaysia Sabah3
Yuri PamungkasInstitut Teknologi Sepuluh Nopember2
Lily IsmainiAfiliasi tidak diketahui2
Vandra KurniawanNational Research and Innovation Agency2
Suluh NormasiwiIndonesian Institute of Sciences2
Abdul KarimHallym University1
Dwinda Mariska PutriCenter for Plant Conservation1
Rusyda FajaraniPoltekkes Kemenkes Jakarta I1
Armelia RamandhaChiba University1
Muhammad Rifqi HaririIndonesian Institute of Sciences1
Arifin Surya Dwipa IrsyamBandung Institute of Technology1
Rina Ratnasih IrwantoBandung Institute of Technology1
Peniwidiyanti PeniwidiyantiNational Research and Innovation Agency1
Kusnadi KusnadiIndonesian Institute of Sciences1
Eni YuhaeniIndonesian Institute of Sciences1
KasrinaAfiliasi tidak diketahui1
Fesy AntinaUniversity of Bengkulu1
Wiguna RahmanAfiliasi tidak diketahui1
Sunarto KalegNational Research and Innovation Agency1
Danardono Agus SumarsonoUniversity of Indonesia1
Alexander Christantho BudimanIndonesian Institute of Sciences1
Djoko Wahyu KarmiadjiNational Research and Innovation Agency1
Mustasyar PerkasaNational Research and Innovation Agency1
Tresna Priyana SoemardiUniversity of Indonesia1
Arief SetyawanNational Research and Innovation Agency1
Rizky Pratama MulyanaNational Research and Innovation Agency1
Arga Agung NugrohoNational Research and Innovation Agency1
Wahyu SulistiyoNational Research and Innovation Agency1
Masripah MasripahNational Research and Innovation Agency1
Ridho DwimansyahNational Research and Innovation Agency1
Makmuri MakmuriNational Research and Innovation Agency1
Wely PasadenaNational Research and Innovation Agency1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device</dc:title>
	<dc:creator>Josephin, Adella</dc:creator>
	<dc:creator>Whulanza, Yudan</dc:creator>
	<dc:creator>Rahman, Siti Fauziyah</dc:creator>
	<dc:creator>Lischer, Kenny</dc:creator>
	<dc:creator>Surya, Muhammad Imam</dc:creator>
	<dc:creator>Martiansyah, Irfan</dc:creator>
	<dc:creator>Rahman, Wiguna</dc:creator>
	<dc:creator>Hashim, Uda</dc:creator>
	<dc:subject xml:lang="en-US">Ganoderma boninense; Infected oil palms; Lab‐on‐chip; Molecular detection; Polymerase chain reaction</dc:subject>
	<dc:description xml:lang="en-US">Detecting Ganoderma boninense in Indonesia is crucial for effectively controlling and mitigating the spread of basal stem disease in oil palm fields. While there is ongoing development of tolerant plants, no such plant has been successfully created yet. Consequently, researchers are actively studying detection methods for Ganoderma boninense. One established and highly accurate approach is the use of polymerase chain reaction (PCR) techniques for molecular detection. However, this method requires time‐consuming sample preparation, which can pose challenges in plantation settings. To address this problem, a portable lab‐on‐chip device has been introduced. This technology enables easy and automatic DNA retrieval from liquid samples by absorbing lysed DNA using magnetic beads. An efficient mechanism for manipulating the magnetic bead within the semiconductor has been successfully implemented. The extraction process typically takes around 15 minutes using a modified methodology on the chip device approach. The chip facilitates the retrieval of two samples with a capacity of 120 µL for each sample. The PCR method was utilized to validate the equivalence of the lab‐on‐chip device extraction to the standard extraction method. This represents a promising alternative for expedited and simplified detection of Ganoderma boninense in field conditions.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Badan Pengelola Dana Perkebunan Kelapa Sawit (BPDPKS)</dc:contributor>
	<dc:date>2024-03-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/83645</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.83645</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 29, No 1 (2024); 33-39</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/83645/38823</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>