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The effect of non‐contact electro capacitive cancer therapy on DMBA‐induced rat breast tumor angiogenesis

Indonesian Journal of Biotechnology · Vol. 29 No. 2 (2024) · hlm. 74–81

DOI
10.22146/ijbiotech.87396
ISSN
2089-2241, 0853-8654
Terbit
2024-06-30
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Alternating Current‐Electric Field (AC‐EF) generated by non‐contact Electro Capacitive Cancer Therapy (ECCT) can inhibit breast tumor growth. However, its effect on breast tumor angiogenesis remains unclear. Since angiogenesis is involved in normal physiology and tumors, it is crucial to investigate the effect of ECCT on normal and breast tumor angiogenesis. Samples consisting of rat breast normal tissue and breast tumors were obtained from the biobank, with tumors induced by 7,12‐dimethylbenz (α) anthracene (DMBA) at 20 mg/kg BW 10 times over five weeks. Meanwhile, ECCT exposure of 150 kHz and 18 Vpp was conducted for 21 days at 10 hours/day. The qPCR method was used for gene expression analysis, while immunohistochemistry used antibody anti‐Vegfr2 that was used to detect Vegfr2 protein expression. Data were analyzed using one‐way ANOVA and t‐tests performed with GraphPad Prism ver.9.5.1 software. The results revealed no impact of ECCT exposure on normal breast tissue angiogenesis. Interestingly, there was a significant increase in the number of blood vessels following the upregulation of Vascular Endothelial Growth Factor Receptor‐2 (Vegfr2) as opposed to its primary signal, Vascular Endothelial Growth Factor‐A (Vegfa). Furthermore, gene expression of Hypoxia Inducible Factor‐1α (Hif1α) and Specificity Protein‐1 (Sp1) was similar to that of the control group, suggesting that Vegfr2‐dependent angiogenesis regulates ECCT‐treated breast tumor angiogenesis.

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  • Universitas Gadjah Mada
  • Universitas Al-Azhar Indonesia
  • IPB University
  • Bandung Institute of Technology
  • Jenderal Soedirman University
  • University of Indonesia
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Rekan penulisOrganisasiArtikel bersama
M. MurwantokoAfiliasi tidak diketahui3
Rumiyati RumiyatiUniversitas Gadjah Mada3
Hanni GarminiaBandung Institute of Technology3
Hera MaheshwariAfiliasi tidak diketahui2
Claude Mona AirinAfiliasi tidak diketahui2
Sarmin SarminUniversitas Gadjah Mada2
Pradnya ParamitaUniversitas Gadjah Mada2
Luthfia Uswatun KhasanahUniversitas Gadjah Mada2
Alfi Rizqi AmaliaUniversitas Gadjah Mada2
Woro Anindito Sri TunjungUniversitas Gadjah Mada2
Yekti Asih PurwestriUniversitas Gadjah Mada2
Sabarinsyah SabarinsyahBandung Institute of Technology2
Suwarno HadisusantoUniversitas Gadjah Mada2
Nastiti WijayantiUniversitas Gadjah Mada2
Selvi AriyunitaUniversitas Gadjah Mada1
Andhika Puspito NugrohoUniversitas Gadjah Mada1
Nita NorikoUniversitas Al-Azhar Indonesia1
Arief PambudiUniversitas Al-Azhar Indonesia1
Siti Mimah RohimahUniversitas Al-Azhar Indonesia1
Dewi ElfidasariUniversitas Al-Azhar Indonesia1
Rizky Nuur Berlianni MulyantoUniversitas Al-Azhar Indonesia1
Jihan Restu BuanaUniversitas Gadjah Mada1
TUTY LASWARDI YUSUFAfiliasi tidak diketahui1
ERIC HAYESAfiliasi tidak diketahui1
LUTHFIRALDA SJAHFIRDIAfiliasi tidak diketahui1
DONDIN SAJUTHIAfiliasi tidak diketahui1
Hadi Sukardi AlikodraAfiliasi tidak diketahui1
Bambang PurwantaraIPB University1
Dondin SajuthiIPB University1
Reviany WidjajakusumaIPB University1
Luthfiralda SjahfirdiUniversity of Indonesia1
Muhammad Naufal Prayitno PutraAfiliasi tidak diketahui1
Muhammad Fariz Ash ShiddiqUniversitas Gadjah Mada1
Rizki Fitrawan YuneldiUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">The effect of non‐contact electro capacitive cancer therapy on DMBA‐induced rat breast tumor angiogenesis</dc:title>
	<dc:creator>Palupi, Endah Sri</dc:creator>
	<dc:creator>Retnoaji, Bambang</dc:creator>
	<dc:creator>Astuti, Pudji</dc:creator>
	<dc:creator>Alamsyah, Firman</dc:creator>
	<dc:creator>Taruno, Warsito Purwo</dc:creator>
	<dc:creator>Pratiwi, Rarastoeti</dc:creator>
	<dc:subject xml:lang="en-US">AC electric field; Angiogenic gene; Breast cancer; Vegfr2‐dependent angiogenesis</dc:subject>
	<dc:description xml:lang="en-US">Alternating Current‐Electric Field (AC‐EF) generated by non‐contact Electro Capacitive Cancer Therapy (ECCT) can inhibit breast tumor growth. However, its effect on breast tumor angiogenesis remains unclear. Since angiogenesis is involved in normal physiology and tumors, it is crucial to investigate the effect of ECCT on normal and breast tumor angiogenesis. Samples consisting of rat breast normal tissue and breast tumors were obtained from the biobank, with tumors induced by 7,12‐dimethylbenz (α) anthracene (DMBA) at 20 mg/kg BW 10 times over five weeks. Meanwhile, ECCT exposure of 150 kHz and 18 Vpp was conducted for 21 days at 10 hours/day. The qPCR method was used for gene expression analysis, while immunohistochemistry used antibody anti‐Vegfr2 that was used to detect Vegfr2 protein expression. Data were analyzed using one‐way ANOVA and t‐tests performed with GraphPad Prism ver.9.5.1 software. The results revealed no impact of ECCT exposure on normal breast tissue angiogenesis. Interestingly, there was a significant increase in the number of blood vessels following the upregulation of Vascular Endothelial Growth Factor Receptor‐2 (Vegfr2) as opposed to its primary signal, Vascular Endothelial Growth Factor‐A (Vegfa). Furthermore, gene expression of Hypoxia Inducible Factor‐1α (Hif1α) and Specificity Protein‐1 (Sp1) was similar to that of the control group, suggesting that Vegfr2‐dependent angiogenesis regulates ECCT‐treated breast tumor angiogenesis.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Indonesian Endowment Fund for Education (LPDP) via BUDI-DN 2019 scholarship</dc:contributor>
	<dc:contributor xml:lang="en-US">Program Pengembangan Teknologi Industri (PPTI) 2018</dc:contributor>
	<dc:date>2024-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/87396</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.87396</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 29, No 2 (2024); 74-81</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/87396/39477</dc:relation>
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	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/87396/28040</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/87396/28041</dc:relation>
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	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/87396/28043</dc:relation>
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	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/87396/28045</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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