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Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer

Indonesian Journal of Biotechnology · Vol. 29 No. 4 (2024) · hlm. 190–197

DOI
10.22146/ijbiotech.92817
ISSN
2089-2241, 0853-8654
Terbit
2024-12-31
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Breast cancer represents the highest number of cancer cases in Indonesia, with triple‐negative breast cancer (TNBC) being a common subtype (10–15%). MicroRNAs play a role in cancer epigenetics and contributing as core factors to the disease. The expression of miR‐143‐3p have been found to be lower in breast cancer samples from Yogyakarta and Central Java. It is known that miR‐143‐3p functions as a tumor suppressor in breast cancer, and its overexpression corresponds with an increased survival rate. The structure of miRNA is quickly degraded, an enhanced delivery system for miRNA is required. Exosomes are indeed emerging as natural delivery agent. A new approach represents that exosomes will be transfected with mimic‐hsa‐miR‐143‐3p yield an exo‐miR. The research aimed to examine how exo‐miR affects viability, migration, and proliferation using 4T1 cell line. The Exo‐Fect‐based method was used to transfect mimic‐hsa‐miR‐143‐3p into exosomes. The MTT assay, wound healing assay, and colony formation assay were used as functional assay. The MTT assay revealed that 7.5 µL/ 250,000 particles exo‐miR obtained a lower percentage of cell viability (58%) than the control (99.7%). The wound healing assay showed that transfection of 37.5 µL/ 1,250,000 particles exo‐miR was able to suppress migration by the percentage of wound closure (67%) compared to the control (100%). Exo‐miR also had a significant (p < 0.001) effect on colony‐forming abilities, as shown by fewer colonies (32) compared to the control (132). This findings demonstrated that exo‐miR represents a promising targeted approach in cancer therapy.

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  • Universitas Gadjah Mada
  • Universitas Kristen Immanuel
  • Hasanuddin University
  • University of Nigeria
  • Universitas Muhammadiyah Semarang
  • Universitas Pattimura
  • Lombok Institute of Technology
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Rekan penulisOrganisasiArtikel bersama
Dewi AgustinaUniversitas Gadjah Mada2
Jecklyn Shindy TemartenanUniversitas Pattimura2
Hairil FiqriLombok Institute of Technology2
Christantie EffendyUniversitas Gadjah Mada1
Andina SetyawatiHasanuddin University1
Mae Sri Hartati WahyuningsihUniversitas Gadjah Mada1
Sam Chidi IbenemeUniversity of Nigeria1
Wasito WasitoUniversitas Gadjah Mada1
Al SupartinahUniversitas Gadjah Mada1
Pinandi Sri PudyaniUniversitas Gadjah Mada1
Sri SuparwitriUniversitas Gadjah Mada1
Aprilia Indra KartikaUniversitas Muhammadiyah Semarang1
Siti Nur ChasanahUniversitas Gadjah Mada1
Akbar Satria FitriawanUniversitas Gadjah Mada1
Dewi Sahfitri TanjungUniversitas Gadjah Mada1
Addin TrirahmantoUniversitas Gadjah Mada1
Heru PradjatmoUniversitas Gadjah Mada1
Teguh AryandonoUniversitas Gadjah Mada1
Ahmad Hamim SadewaUniversitas Gadjah Mada1
Sarah Puspita AtmajaUniversitas Kristen Immanuel1
Ellsya Angeline RawarUniversitas Kristen Immanuel1
Ani KristiyaniUniversitas Kristen Immanuel1
Christine PatramurtiSanata Dharma University1
Hevi WihadmadyatamiUniversitas Gadjah Mada1
Ulayatul KustiatiUniversity of Brawijaya1
Dwi Liliek KusindartaUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer</dc:title>
	<dc:creator>Nilasari, Fita</dc:creator>
	<dc:creator>Haryana, Sofia Mubarika</dc:creator>
	<dc:creator>Nugrahaningsih, Dwi Aris Agung</dc:creator>
	<dc:creator>Satriyo, Pamungkas Bagus</dc:creator>
	<dc:subject xml:lang="en-US">Exosome; Migration; mimic‐hsa‐miR‐143‐3p; Proliferation; TNBC; Viability</dc:subject>
	<dc:description xml:lang="en-US">Breast cancer represents the highest number of cancer cases in Indonesia, with triple‐negative breast cancer (TNBC) being a common subtype (10–15%). MicroRNAs play a role in cancer epigenetics and contributing as core factors to the disease. The expression of miR‐143‐3p have been found to be lower in breast cancer samples from Yogyakarta and Central Java. It is known that miR‐143‐3p functions as a tumor suppressor in breast cancer, and its overexpression corresponds with an increased survival rate. The structure of miRNA is quickly degraded, an enhanced delivery system for miRNA is required. Exosomes are indeed emerging as natural delivery agent. A new approach represents that exosomes will be transfected with mimic‐hsa‐miR‐143‐3p yield an exo‐miR. The research aimed to examine how exo‐miR affects viability, migration, and proliferation using 4T1 cell line. The Exo‐Fect‐based method was used to transfect mimic‐hsa‐miR‐143‐3p into exosomes. The MTT assay, wound healing assay, and colony formation assay were used as functional assay. The MTT assay revealed that 7.5 µL/ 250,000 particles exo‐miR obtained a lower percentage of cell viability (58%) than the control (99.7%). The wound healing assay showed that transfection of 37.5 µL/ 1,250,000 particles exo‐miR was able to suppress migration by the percentage of wound closure (67%) compared to the control (100%). Exo‐miR also had a significant (p &lt; 0.001) effect on colony‐forming abilities, as shown by fewer colonies (32) compared to the control (132). This findings demonstrated that exo‐miR represents a promising targeted approach in cancer therapy.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Center for Higher Education Funding (BPPT)</dc:contributor>
	<dc:contributor xml:lang="en-US">Indonesia Endowment Fund for Education (LPDP)</dc:contributor>
	<dc:date>2024-12-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/92817</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.92817</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 29, No 4 (2024); 190-197</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/92817/40788</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/92817/31060</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/92817/32838</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>