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Molecular analyses of antimicrobial resistance characteristic of Klebsiella pneumoniae strain CJK 500 2.1.2 isolated from Cikijing River

Indonesian Journal of Biotechnology · Vol. 31 No. 2 (2026) · hlm. 137–149

DOI
10.22146/ijbiotech.110454
ISSN
2089-2241, 0853-8654
Terbit
2026-06-30
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Many areas in Indonesia face severe pollution from copper and antibiotics, impacting microbial diversity and underscoring the need for effective remediation strategies. Harnessing of copper- and antibiotic-resistant bacteria has become a promising approach. This study evaluates the antimicrobial resistance (AMR) profile of copper-accumulating Klebsiella pneumoniae strain CJK 500 2.1.2 as a bioremediation agent candidate. Whole-genome analysis of K. pneumoniae CJK 500 2.1.2 revealed multiple AMR genes, primarily encoding efflux pumps, with one fieF gene facilitating resistance to heavy metals. The disk-diffusion assay highlighted multidrug-resistance traits, regardless of CuSO4. Notably, increased cefoxitin resistance in the presence of CuSO4 and intermediate susceptibility to tetracycline were observed, which was corroborated by a broth microdilution assay. The genome analysis identified 22 virulence factors (VFs) and 16 insertion sequences (ISs), with some ISs located near AMR and VF genes, suggesting possible horizontal gene transfer (HGT). Overall, K. pneumoniae CJK 500 2.1.2 showed a potential as a model study for bioremediation research on genetically engineered microorganism (GEM). Through risk assessment, GEMs have the possibility to be applied to copper and antibiotics bioremediation.

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  • Pelita Harapan University
  • Cenderawasih University
  • Australian National University
  • Padjadjaran University
  • Universitas Gadjah Mada
  • University of Indonesia
  • The University of Queensland
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Hans VictorPelita Harapan University2
Candra Yulius TahyaPelita Harapan University2
Aaron HasthosaputroPelita Harapan University1
Lucia KusumawatiAustralian National University1
Argorby SimanullangCenderawasih University1
Naomi DwanyCenderawasih University1
Ruth Y. PurbaCenderawasih University1
Melany DjamiCenderawasih University1
Elysabeth SimatupangPelita Harapan University1
Pahotkon PurbaPelita Harapan University1
Sherina JulianaPelita Harapan University1
Yohana Delvia AyustinPelita Harapan University1
Vania Austine Callista TimotiusPelita Harapan University1
Ruben Patrick AdhiwijayaPelita Harapan University1
Eunike Bunga MarvellaAfiliasi tidak diketahui1
Polin Parulian AmbaritaPelita Harapan University1
Vannesa El Shaday Ruth AdvenitaPelita Harapan University1
Sintia Y. RahmawatiPelita Harapan University1
Damai Yanti ManaluPelita Harapan University1
Elisabeth M.T. RidoPelita Harapan University1
Rusliani S. NoveraPelita Harapan University1
Apriliana AngelicaPelita Harapan University1
Dwi P. MaharajaPelita Harapan University1
Stefani SitumorangPelita Harapan University1
Budi SetiabudiawanPadjadjaran University1
Juwalita SurapsariAfiliasi tidak diketahui1
Melissa Stephanie KartjitoUniversitas Gadjah Mada1
Nova Lidia SitorusAfiliasi tidak diketahui1
Charisma DilantikaAfiliasi tidak diketahui1
Ray Wagiu BasrowiUniversity of Indonesia1
. PATRICIAAfiliasi tidak diketahui1
YENNY SORAYAPelita Harapan University1
ABYATAR HUGO BASKOROPelita Harapan University1
FigoPelita Harapan University1
Patricia CelineThe University of Queensland1
Melanie CorneliaPelita Harapan University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Molecular analyses of antimicrobial resistance characteristic of Klebsiella pneumoniae strain CJK 500 2.1.2 isolated from Cikijing River</dc:title>
	<dc:creator>Irawati, Wahyu</dc:creator>
	<dc:creator>Ong, Michael Kayne</dc:creator>
	<dc:creator>Purnomo, Jonathan Suciono</dc:creator>
	<dc:creator>Jo, Juandy</dc:creator>
	<dc:subject xml:lang="en-US">Antimicrobial resistance genes; Bioremediation; Copper; Klebsiella pneumoniae; Mobile elements</dc:subject>
	<dc:description xml:lang="en-US">Many areas in Indonesia face severe pollution from copper and antibiotics, impacting microbial diversity and underscoring the need for effective remediation strategies. Harnessing of copper- and antibiotic-resistant bacteria has become a promising approach. This study evaluates the antimicrobial resistance (AMR) profile of copper-accumulating Klebsiella pneumoniae strain CJK 500 2.1.2 as a bioremediation agent candidate. Whole-genome analysis of K. pneumoniae CJK 500 2.1.2 revealed multiple AMR genes, primarily encoding efflux pumps, with one fieF gene facilitating resistance to heavy metals. The disk-diffusion assay highlighted multidrug-resistance traits, regardless of CuSO4. Notably, increased cefoxitin resistance in the presence of CuSO4 and intermediate susceptibility to tetracycline were observed, which was corroborated by a broth microdilution assay. The genome analysis identified 22 virulence factors (VFs) and 16 insertion sequences (ISs), with some ISs located near AMR and VF genes, suggesting possible horizontal gene transfer (HGT). Overall, K. pneumoniae CJK 500 2.1.2 showed a potential as a model study for bioremediation research on genetically engineered microorganism (GEM). Through risk assessment, GEMs have the possibility to be applied to copper and antibiotics bioremediation.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2026-06-30</dc:date>
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	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/110454</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.110454</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 31, No 2 (2026); 137–149</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/110454/44410</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/110454/40460</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/110454/40461</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/110454/40462</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/110454/40463</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/110454/40464</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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