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Evaluation of synthetic‐gene recombinant LipL32 antigen for IgM ELISA detection of Leptospira infection

Indonesian Journal of Biotechnology · Vol. 30 No. 4 (2025) · hlm. 226–235

DOI
10.22146/ijbiotech.111102
ISSN
2089-2241, 0853-8654
Terbit
2025-12-31
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Leptospirosis presents with nonspecific clinical features and requires time‐consuming laboratory tests for gold standard diagnosis. This study aims to design and characterize the recombinant LipL32 from synthetic gene and assess its performance as an antigen for detecting leptospirosis. The antigen was developed by cloning the LipL32 gene conserved portion of Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi. The immunoinformatic was used to characterize the developed rLipL32. Western blot results using anti‐histidine revealed a band of rLipL32 protein at ~40 kDa. Subsequently, it was used to examine the IgM antibody on human sera by using ELISA. The IgM‐LipL32 ELISA was evaluated using 67‐positive and 25‐negative sera and compared with a commercial ELISA. With a cut‐off value of 0.8, it showed 85.7% sensitivity, 83.3% specificity, a 48% positive prediction value (PPV), and 97% negative prediction value (NPV), indicating modest performance compared to existing commercial kits. The rLipL32 is a potential antigen for detecting IgM using ELISA; however, for use in low incidence areas, a confirmation test is crucial.

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  • Universitas Gadjah Mada
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Rekan penulisOrganisasiArtikel bersama
Michael KannAfiliasi tidak diketahui7
Edy MeiyantoAfiliasi tidak diketahui4
Tri WibawaUniversitas Gadjah Mada4
Hevi WihadmadyatamiAfiliasi tidak diketahui4
Warih Pulung NugrahaniAfiliasi tidak diketahui3
Krisna Noli AndrianAfiliasi tidak diketahui3
Sari HaryantiAfiliasi tidak diketahui2
Suwijiyo PramonoAfiliasi tidak diketahui2
Diana SavitriAfiliasi tidak diketahui2
Irhamna PutriAfiliasi tidak diketahui2
Medania PurwaningrumAfiliasi tidak diketahui2
Asri Mega PutriAfiliasi tidak diketahui2
Nindya Budiana PutriAfiliasi tidak diketahui2
Sidiq PurwokoAfiliasi tidak diketahui2
Hera NirwatiAfiliasi tidak diketahui2
S. SutaryoAfiliasi tidak diketahui2
Nenny Sri MulyaniAfiliasi tidak diketahui2
Titis WidowatiAfiliasi tidak diketahui2
Purnomo HadiAfiliasi tidak diketahui2
Rinawati RinawatiAfiliasi tidak diketahui1
E SuharyantoAfiliasi tidak diketahui1
Muhammad AdnanAfiliasi tidak diketahui1
Matin NuhamunadaAfiliasi tidak diketahui1
Lisna HidayatiAfiliasi tidak diketahui1
Abdul Rahman SiregarAfiliasi tidak diketahui1
Jaka WidadaUniversitas Gadjah Mada1
Shinta HartantoAfiliasi tidak diketahui1
Sofia MubarikaAfiliasi tidak diketahui1
Camelia HerdiniAfiliasi tidak diketahui1
Bambang HariwiyantoAfiliasi tidak diketahui1
Akira HosoyamaAfiliasi tidak diketahui1
Atsushi YamazoeAfiliasi tidak diketahui1
Hideaki NojiriAfiliasi tidak diketahui1
Aushia Tanzih Al HaqAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Evaluation of synthetic‐gene recombinant LipL32 antigen for IgM ELISA detection of Leptospira infection</dc:title>
	<dc:creator>Widiastuti, Dyah</dc:creator>
	<dc:creator>Paramita, Dewi Kartikawati</dc:creator>
	<dc:creator>Murwanti, Retno</dc:creator>
	<dc:creator>Kusrini, Ina</dc:creator>
	<dc:creator>Wijayanti, Nastiti</dc:creator>
	<dc:creator>Haryanto, Aris</dc:creator>
	<dc:subject xml:lang="en-US">Detection; ELISA; Leptospirosis; LipL32; Recombinant</dc:subject>
	<dc:description xml:lang="en-US">Leptospirosis presents with nonspecific clinical features and requires time‐consuming laboratory tests for gold standard diagnosis. This study aims to design and characterize the recombinant LipL32 from synthetic gene and assess its performance as an antigen for detecting leptospirosis. The antigen was developed by cloning the LipL32 gene conserved portion of Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi. The immunoinformatic was used to characterize the developed rLipL32. Western blot results using anti‐histidine revealed a band of rLipL32 protein at ~40 kDa. Subsequently, it was used to examine the IgM antibody on human sera by using ELISA. The IgM‐LipL32 ELISA was evaluated using 67‐positive and 25‐negative sera and compared with a commercial ELISA. With a cut‐off value of 0.8, it showed 85.7% sensitivity, 83.3% specificity, a 48% positive prediction value (PPV), and 97% negative prediction value (NPV), indicating modest performance compared to existing commercial kits. The rLipL32 is a potential antigen for detecting IgM using ELISA; however, for use in low incidence areas, a confirmation test is crucial.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Directorate General of Health Human Resources of the Indonesian Ministry of Health</dc:contributor>
	<dc:date>2025-12-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/111102</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.111102</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 30, No 4 (2025); 226–235</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/111102/43169</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/111102/40852</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/111102/40853</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/111102/40854</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/111102/40859</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/111102/40860</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/111102/40861</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/111102/40862</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/111102/40863</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/111102/40864</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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