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Orchid‐associated endophytic Bacillus mediates Fusarium suppression and promotes in vitro regeneration of banana plantlets via culture supernatant

Indonesian Journal of Biotechnology · Vol. 30 No. 4 (2025) · hlm. 236–246

DOI
10.22146/ijbiotech.111727
ISSN
2089-2241, 0853-8654
Terbit
2025-12-31
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The application of Fusarium‐antagonistic endophytic bacteria with plant growth‐promoting traits offers an effective method to enhance the success of banana plantlet tissue culture while combating Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense Tropical Race 4 (FocTR4) (VCG 01213). This study evaluates the endophytic bacterium AP3311, isolated from healthy banana roots in direct association with orchid roots. AP3311 exhibited strong antagonism toward FocTR4, hyphal colonization ability, and multiple growth‐promoting activities, including phosphate solubilization, nitrogen fixation and auxin production. 16S rRNA gene sequencing identified that AP3311 belongs to the genus Bacillus, while metabarcoding analysis revealed that Bacillus species dominate the root microbiomes of both bananas and orchids. The bacterial supernatants stimulated root development and leaf growth in vitro. Metabolomic profiling indicated that antimicrobial compounds, together with plant growth regulators, promoted both root and shoot growth. Overall, the research demonstrates that Bacillus sp. AP3311 and its supernatants are valuable components in banana tissue culture, providing the dual benefits of plant growth promotion and effective disease control.

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  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Tokyo University of Agriculture
  • Institut Pertanian Stiper
  • Universitas Muhammadiyah Pontianak
  • Universitas Islam Negeri Sultan Maulana Hasanuddin Banten
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Rekan penulisOrganisasiArtikel bersama
Rina Sri KasiamdariUniversitas Gadjah Mada5
Endang SemiartiUniversitas Gadjah Mada5
Ardaning NurilianiUniversitas Gadjah Mada4
Tri Rini NuringtyasUniversitas Gadjah Mada4
Arif MuazamUniversitas Gadjah Mada3
Setyorini WidyayantiNational Research and Innovation Agency3
Langkah SembiringUniversitas Gadjah Mada3
Didik IndradewaUniversitas Gadjah Mada3
Arif WibowoUniversitas Gadjah Mada3
Tri JokoUniversitas Gadjah Mada3
Alan SoffanUniversitas Gadjah Mada3
Eko Agus SuyonoUniversitas Gadjah Mada3
Endah RetnaningrumUniversitas Gadjah Mada3
Woro Anindito Sri TunjungUniversitas Gadjah Mada3
Purnomo PurnomoUniversitas Gadjah Mada2
Adib Fakhruddin YusufUniversitas Gadjah Mada2
Purnomo PurnomoUniversitas Gadjah Mada2
Ganies Riza AristyaUniversitas Gadjah Mada2
Tuty ArisuryantiUniversitas Gadjah Mada2
Yudhistira NugrahaNational Research and Innovation Agency2
Kristamtini KristamtiniNational Research and Innovation Agency2
Keiko T. NatsuakiTokyo University of Agriculture2
Charlie Ester de FretesNational Research and Innovation Agency2
Fitri NadifahUniversitas Gadjah Mada2
Radhiyah Mardhiyah HamidUniversitas Gadjah Mada2
Rifqi Zahroh JanatunaimUniversitas Gadjah Mada2
Rarastoeti PratiwiUniversitas Gadjah Mada2
Ari IndriantoUniversitas Gadjah Mada2
Donny WidiantoUniversitas Gadjah Mada2
Alfino SebastianUniversitas Gadjah Mada2
Wahyu Aristyaning PutriUniversitas Gadjah Mada2
Hanum Mukti RahayuUniversitas Muhammadiyah Pontianak2
Anis Uswatun KhasanahUniversitas Islam Negeri Sultan Maulana Hasanuddin Banten2
Yuni SineUniversitas Gadjah Mada2
Yohana Theresia Maria AstutiInstitut Pertanian Stiper2
Diah RachmawatiUniversitas Gadjah Mada2

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Orchid‐associated endophytic Bacillus mediates Fusarium suppression and promotes in vitro regeneration of banana plantlets via culture supernatant</dc:title>
	<dc:creator>Sasongko, Aries Bagus</dc:creator>
	<dc:creator>Purwestri, Yekti Asih</dc:creator>
	<dc:creator>Daryono, Budi Setiadi</dc:creator>
	<dc:creator>Subandiyah, Siti</dc:creator>
	<dc:subject xml:lang="en-US">Endophytic Bacillus; Fusarium oxysporum f. sp. cubense Tropical Race 4; In vitro regeneration; Bacterial culture super‐ natant; Metabolomics</dc:subject>
	<dc:description xml:lang="en-US">The application of Fusarium‐antagonistic endophytic bacteria with plant growth‐promoting traits offers an effective method to enhance the success of banana plantlet tissue culture while combating Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense Tropical Race 4 (FocTR4) (VCG 01213). This study evaluates the endophytic bacterium AP3311, isolated from healthy banana roots in direct association with orchid roots. AP3311 exhibited strong antagonism toward FocTR4, hyphal colonization ability, and multiple growth‐promoting activities, including phosphate solubilization, nitrogen fixation and auxin production. 16S rRNA gene sequencing identified that AP3311 belongs to the genus Bacillus, while metabarcoding analysis revealed that Bacillus species dominate the root microbiomes of both bananas and orchids. The bacterial supernatants stimulated root development and leaf growth in vitro. Metabolomic profiling indicated that antimicrobial compounds, together with plant growth regulators, promoted both root and shoot growth. Overall, the research demonstrates that Bacillus sp. AP3311 and its supernatants are valuable components in banana tissue culture, providing the dual benefits of plant growth promotion and effective disease control.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Beasiswa Pendidikan Indonesia – LPDP 2021 Ministry of Education and Culture of the Republic of Indonesia</dc:contributor>
	<dc:date>2025-12-31</dc:date>
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	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/111727</dc:identifier>
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	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 30, No 4 (2025); 236–246</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
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	<dc:rights xml:lang="en-US">Copyright (c) 2025 The Author(s)</dc:rights>
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