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Detection of homologous plastic PET‐degrading enzyme‐encoding DNA from enriched plastic‐contaminated soil samples

Indonesian Journal of Biotechnology · Vol. 31 No. 1 (2026) · hlm. 62–71

DOI
10.22146/ijbiotech.112331
ISSN
2089-2241, 0853-8654
Terbit
2026-03-31
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Piscinibacter sakaiensis, first isolated in Japan, is the only well‐characterized bacterium known to possess both PETase and MHETase, enabling complete polyethylene terephthalate (PET) degradation. To date, no additional habitats for the species have been reported. This study aims to identify homologous PETase and MHETase DNA from plastic‐contaminated landfill soils in Indonesia. Enrichment cultures were established from soil samples collected at Galuga (Bogor) and Cipeucang (South Tangerang). PCR amplification and sequencing revealed a full‐length MHETase homolog (G2MHETase, 1,812 bp) from Galuga, showing 99.4% and 99.3% nucleotide identity to MHETase from P. sakaiensis and Delftia sp. respectively. The deduced amino acid sequence shared 98.5% identity with both. In contrast, a partial PETase homolog (502 bp of 873 bp) was amplified from the Cipeucang sample, displaying 96 and 93% amino acid identity to PETase from P. sakaiensis and P. gummiphilus respectively. Nanophore NGS analysis of bacterial diversity indicated distinct microbial community profiles between the two sites. Rare taxa potentially associated with the detected genes included P. gummiphilus, Delftia sp., Delftia tsuruhatensis and Xenophilus aerolatus from Galuga, and Piscinibacter and Acidovorax from Cipeucang. These findings demonstrate the feasibility of detecting homologous PET degrading enzyme genes from plastic‐contaminated soils using PCR‐based approaches.

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  • Universitas Syiah Kuala
  • Ministry of Marine Affairs and Fisheries
  • Politeknik Negeri Balikpapan
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  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Gabriela Christy SabbathiniAfiliasi tidak diketahui11
AbinawantoAfiliasi tidak diketahui6
Agustin Krisna WardaniAfiliasi tidak diketahui4
Lina MulyawatiAfiliasi tidak diketahui3
Fibria KaswinarniAfiliasi tidak diketahui2
AMARILA MALIKAfiliasi tidak diketahui2
Fairuz Andini FatiningtyasAfiliasi tidak diketahui2
Riswanto NapitupuluAfiliasi tidak diketahui2
Naufal AlkhalisUniversitas Syiah Kuala1
Haekal Azief HaridhiUniversitas Syiah Kuala1
Cut Nadilla MaretnaUniversitas Syiah Kuala1
Nur FadliUniversitas Syiah Kuala1
Yudi HaditiarUniversitas Syiah Kuala1
Muhammad NandaUniversitas Syiah Kuala1
Kris HandokoMinistry of Marine Affairs and Fisheries1
Intan MalayanaUniversitas Syiah Kuala1
Arsa Cindy SafitriUniversitas Syiah Kuala1
HusainiAfiliasi tidak diketahui1
Asnath Maria FuahAfiliasi tidak diketahui1
Yuni Cahya EndrawatiAfiliasi tidak diketahui1
Winarno WinarnoAfiliasi tidak diketahui1
Nurwahidah JamalPoliteknik Negeri Balikpapan1
Nur Rahmah Intan SariAfiliasi tidak diketahui1
Pevy Syntia DewiAfiliasi tidak diketahui1
Ade IhtiarAfiliasi tidak diketahui1
Labib AnwarAfiliasi tidak diketahui1
Atip NurwahyunaniAfiliasi tidak diketahui1
Luhur Pujo SantosoAfiliasi tidak diketahui1
Eny EnawatyAfiliasi tidak diketahui1
Rini MuhariniAfiliasi tidak diketahui1
Hanna Rohmatul MaulidiyahAfiliasi tidak diketahui1
Risya SasriAfiliasi tidak diketahui1
Erlina ErlinaAfiliasi tidak diketahui1
Restika PamungkasihAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Detection of homologous plastic PET‐degrading enzyme‐encoding DNA from enriched plastic‐contaminated soil samples</dc:title>
	<dc:creator>Wulandari, Sri Rezeki</dc:creator>
	<dc:creator>Sabbthini, Gabriela Christy</dc:creator>
	<dc:creator>Trinugroho, Joko Pebrianto</dc:creator>
	<dc:creator>Nurhayati, Niknik</dc:creator>
	<dc:creator>Ulfah, Maria</dc:creator>
	<dc:creator>Helianti, Is</dc:creator>
	<dc:subject xml:lang="en-US">MHETase; PCR; PETase; Plastics contaminated soil sample; Sequence homology</dc:subject>
	<dc:description xml:lang="en-US">Piscinibacter sakaiensis, first isolated in Japan, is the only well‐characterized bacterium known to possess both PETase and MHETase, enabling complete polyethylene terephthalate (PET) degradation. To date, no additional habitats for the species have been reported. This study aims to identify homologous PETase and MHETase DNA from plastic‐contaminated landfill soils in Indonesia. Enrichment cultures were established from soil samples collected at Galuga (Bogor) and Cipeucang (South Tangerang). PCR amplification and sequencing revealed a full‐length MHETase homolog (G2MHETase, 1,812 bp) from Galuga, showing 99.4% and 99.3% nucleotide identity to MHETase from P. sakaiensis and Delftia sp. respectively. The deduced amino acid sequence shared 98.5% identity with both. In contrast, a partial PETase homolog (502 bp of 873 bp) was amplified from the Cipeucang sample, displaying 96 and 93% amino acid identity to PETase from P. sakaiensis and P. gummiphilus respectively. Nanophore NGS analysis of bacterial diversity indicated distinct microbial community profiles between the two sites. Rare taxa potentially associated with the detected genes included P. gummiphilus, Delftia sp., Delftia tsuruhatensis and Xenophilus aerolatus from Galuga, and Piscinibacter and Acidovorax from Cipeucang. These findings demonstrate the feasibility of detecting homologous PET degrading enzyme genes from plastic‐contaminated soils using PCR‐based approaches.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">National Research and Innovation Agency (BRIN)</dc:contributor>
	<dc:contributor xml:lang="en-US">LPDP</dc:contributor>
	<dc:date>2026-03-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/112331</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.112331</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 31, No 1 (2026); 62-71</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/112331/43712</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/112331/43711</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/112331/41564</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/112331/41565</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>