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Generation of a bivalent dengue virus-like particle targeting DENV-1 and DENV-2 in Pichia pastoris

Indonesian Journal of Biotechnology · Vol. 31 No. 2 (2026) · hlm. 119–127

DOI
10.22146/ijbiotech.114770
ISSN
2089-2241, 0853-8654
Terbit
2026-06-30
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Dengue remains a major global health concern, with current licensed vaccines offering variable protection across the four virus serotypes and posing safety considerations related to antibody-dependent enhancement (ADE). To contribute toward improved vaccine design, we developed a bivalent virus-like particle (VLP) targeting DENV-1 and DENV-2, the two most prevalent serotypes in Indonesia. Consensus envelope (CE) protein sequences from Indonesian DENV-1 and DENV-2 strains were codon-optimized and co-expressed in Pichia pastoris GS115 using the AOX1 promoter. The expressed proteins were solubilized from membrane fractions under denaturing conditions, purified via Ni2+-affinity chromatography, and subjected to urea gradient dialysis for VLP assembly. Transmission electron microscopy confirmed the formation of spherical VLPs. Western blot analysis confirmed expression and purification via anti-His detection, and supported antigenic integrity of the envelope proteins as recognized by anti-dengue E antibodies. The study demonstrates the feasibility of using P. pastoris for chimeric dengue VLP production based on regionally relevant strains. The findings support the development of serotype-focused VLP platforms as modular components in the development of future tetravalent dengue vaccines.

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  • Padjadjaran University
  • Bandung Institute of Technology
  • Sekolah Tinggi Farmasi Indonesia
  • Dr. Hasan Sadikin General Hospital
  • PT Bio Farma (Indonesia)
  • Universitas Bhakti Kencana
  • Sunan Gunung Djati State Islamic University Bandung
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Rekan penulisOrganisasiArtikel bersama
Muhammad YusufPadjadjaran University8
Dewi AstrianySekolah Tinggi Farmasi Indonesia4
Rima HandiyaniPadjadjaran University3
Idar IdarUniversitas Bhakti Kencana3
Iman Permana MaksumPadjadjaran University2
Clara ClaudiaAfiliasi tidak diketahui2
Elsa DestianaAfiliasi tidak diketahui2
Khotibul UmamSekolah Tinggi Farmasi Indonesia1
Yani SuryaniSunan Gunung Djati State Islamic University Bandung1
Opik TaupiqurrohmanSekolah Tinggi Analis Bakti Asih1
Sukma NuswantaraOrganisasi Riset Hayati dan Lingkungan1
DEBBIE SOEFIE RETNONINGRUMBandung Institute of Technology1
Patricia Gita NaullyBandung Institute of Technology1
Elvi RestiawatyBandung Institute of Technology1
Dessy NataliaBandung Institute of Technology1
Wardono NiloperbowoBandung Institute of Technology1
Ernawati Arifin Giri-RachmanBandung Institute of Technology1
Rahmaniar MulyaniPadjadjaran University1
Khomairi HasanUniversitas Jenderal Achmad Yani1
Mamlikatu Ilmi AzizahPadjadjaran University1
Wanda DestiaraniPadjadjaran University1
Ahmad Fariz MaulanaPadjadjaran University1
Hesti Lina WiraswatiPadjadjaran University1
Nisa FauziahPadjadjaran University1
Fauzian Giansyah RohmatullohPadjadjaran University1
Siti SoidahPadjadjaran University1
Sari SyahruniPadjadjaran University1
Henry ChandraPadjadjaran University1
Dhiya Ulhaq SalsabilaPadjadjaran University1
Bachti AlisjahbanaDr. Hasan Sadikin General Hospital1
Leonardus WiydatmokoDr. Hasan Sadikin General Hospital1
Basti AndriyokoDr. Hasan Sadikin General Hospital1
Anita YuwitaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Generation of a bivalent dengue virus-like particle targeting DENV-1 and DENV-2 in Pichia pastoris</dc:title>
	<dc:creator>Kusumawardani, Shinta</dc:creator>
	<dc:creator>Yusuf, Muhammad</dc:creator>
	<dc:creator>Yosua, Yosua</dc:creator>
	<dc:creator>Syafrudin, Elza Nurrachmanita</dc:creator>
	<dc:creator>Baroroh, Umi</dc:creator>
	<dc:creator>Nurainy, Neni</dc:creator>
	<dc:creator>Subroto, Toto</dc:creator>
	<dc:subject xml:lang="en-US">Dengue virus; DENV-1/DENV-2; Envelope protein; Pichia pastoris; Virus-like particle</dc:subject>
	<dc:description xml:lang="en-US">Dengue remains a major global health concern, with current licensed vaccines offering variable protection across the four virus serotypes and posing safety considerations related to antibody-dependent enhancement (ADE). To contribute toward improved vaccine design, we developed a bivalent virus-like particle (VLP) targeting DENV-1 and DENV-2, the two most prevalent serotypes in Indonesia. Consensus envelope (CE) protein sequences from Indonesian DENV-1 and DENV-2 strains were codon-optimized and co-expressed in Pichia pastoris GS115 using the AOX1 promoter. The expressed proteins were solubilized from membrane fractions under denaturing conditions, purified via Ni2+-affinity chromatography, and subjected to urea gradient dialysis for VLP assembly. Transmission electron microscopy confirmed the formation of spherical VLPs. Western blot analysis confirmed expression and purification via anti-His detection, and supported antigenic integrity of the envelope proteins as recognized by anti-dengue E antibodies. The study demonstrates the feasibility of using P. pastoris for chimeric dengue VLP production based on regionally relevant strains. The findings support the development of serotype-focused VLP platforms as modular components in the development of future tetravalent dengue vaccines.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">The authors thank PT Bio Farma (Persero) for their support and research collaboration. Special thanks to the Research Center for Molecular Biotechnology and Bioinformatics for providing laboratory infrastructure and technical assistance during molecular c</dc:contributor>
	<dc:date>2026-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/114770</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.114770</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 31, No 2 (2026); 119–127</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/114770/44382</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>