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Anti-Aging Activity Of Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin

Indonesian Journal of Cancer Chemoprevention · Vol. 7 No. 2 (2016) · hlm. 49–53

DOI
10.14499/indonesianjcanchemoprev7iss2pp49-53
ISSN
2355-8989, 2088-0197
Terbit
2016-06-30
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

Degenerative disease caused by decreasing organ function in old people has become the biggest death cause in the world. This aging process marked by senescence activity. An anti-aging agent from the medicinal plant has high potency to be developed, such as pumpkin seed (Cucurbita moschata) which contain tocopherol as antioxidant. The aim of this study is to investigate the anti-aging effect of pumpkin seed extract (PSE) on NIH 3T3 fibroblast normal cell induced by doxorubicin. This anti-aging effect was observed using MTT assay continued by SA- βgal (Senescence-Associated Beta-Galactosidase) activity detection test. The stability of molecular interaction between tocopherol and doxorubicin to CYP 3A4 as the oxidase enzyme was conducted using molecular docking. Based on in vitro test, PSE is not cytotoxic to NIH 3T3. PSE at the dose of 100,200,400, and 800 μg/mL decreased % cell senescence by 2,77; 4,5; 6; and 18 times respectively. Meanwhile, in silico test indicated that tocopherol (-107,409) has a higher interaction to CYP 3A4 compared to doxorubicin (-70,52). Both compounds have a similar binding site in Leu 364; Phe 435; Pro 434; Cys 442; Ile 369; Thr 309; dan Ala 305. The overall result of this study showed that PSE has anti-aging effect by decreasing SA- βgal activity. This anti-aging activity possibly due to the interaction between tocopherol to CYP 3A4 based on molecular docking.Keywords: anti-aging, Cucurbita moschata, tocopherol, SA-βgal, molecular docking

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  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Sultan Agung Islamic University
  • Sunan Kalijaga State Islamic University Yogyakarta
  • Universitas Wahid Hasyim
  • Padjadjaran University
  • Sebelas Maret University
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Rekan penulisOrganisasiArtikel bersama
Edy MeiyantoUniversitas Gadjah Mada16
Sri HandayaniIndonesian Institute of Sciences6
Ratna Asmah SusidartiUniversitas Gadjah Mada6
Rohmad Yudi UtomoUniversitas Gadjah Mada6
Adam HermawanUniversitas Gadjah Mada4
Annisa NovarinaUniversitas Gadjah Mada3
Dhania NovitasariUniversitas Gadjah Mada3
Zalinar UdinIndonesian Institute of Sciences2
Yogi ErtantoUniversitas Gadjah Mada2
Muthi IkawatiUniversitas Gadjah Mada2
Laili Nailul MunaSunan Kalijaga State Islamic University Yogyakarta2
Rumiyati RumiyatiUniversitas Gadjah Mada2
Argandita MeiftasariUniversitas Gadjah Mada2
Hilyatul FadliyahUniversitas Gadjah Mada2
Prisnu TirtanirmalaUniversitas Gadjah Mada2
Devi Nisa HidayatiUniversitas Wahid Hasyim2
Imroatus SholihahUniversitas Gadjah Mada2
Meirizky Zulharini S.Universitas Gadjah Mada2
Nindya Budiana PutriUniversitas Gadjah Mada2
Puspa Dewi LotulungIndonesian Institute of Sciences1
Akhmad DarmawanIndonesian Institute of Sciences1
Rosa AdelinaAfiliasi tidak diketahui1
Maria Dwi SupriyatiAfiliasi tidak diketahui1
Dwi Ana NawangsariAfiliasi tidak diketahui1
Nur Fitra SariUniversitas Gadjah Mada1
Dhiya Ulhaq SalsabilaPadjadjaran University1
Nisa Ul HasanahUniversitas Gadjah Mada1
Ahmad Syauqy TafrihaniUniversitas Gadjah Mada1
Ummi Maryam ZulfinUniversitas Gadjah Mada1
Ratih Kurnia WardaniUniversitas Gadjah Mada1
Agung PutraSultan Agung Islamic University1
Iffan AlifSultan Agung Islamic University1
Anif Nur ArtantiSebelas Maret University1
Januar Caesar W.P.Universitas Gadjah Mada1
J. W.Universitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Anti-Aging Activity Of  Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin</dc:title>
	<dc:creator>Muntafiah, Laeli</dc:creator>
	<dc:creator>Shabrina, Bani Adlina</dc:creator>
	<dc:creator>Sulistyowati, Dwi</dc:creator>
	<dc:creator>Asshagab, Murah Riski Novi</dc:creator>
	<dc:creator>Jenie, Riris Istighfari</dc:creator>
	<dc:description xml:lang="en-US">Degenerative disease caused by decreasing organ function in old people has become the biggest death cause in the world. This aging process marked by senescence activity. An anti-aging agent from the medicinal plant has high potency to be developed, such as pumpkin seed (Cucurbita moschata) which contain tocopherol as antioxidant. The aim of this study is to investigate the anti-aging effect of pumpkin seed extract (PSE) on NIH 3T3 fibroblast normal cell induced by doxorubicin. This anti-aging effect was observed using MTT assay continued by SA- βgal (Senescence-Associated Beta-Galactosidase) activity detection test. The stability of  molecular interaction between tocopherol and doxorubicin to CYP 3A4 as the oxidase enzyme was conducted using molecular docking. Based on in vitro test, PSE is not cytotoxic to NIH 3T3. PSE at the dose of 100,200,400, and 800 μg/mL decreased % cell senescence by 2,77; 4,5; 6; and 18 times respectively. Meanwhile, in silico test indicated that tocopherol (-107,409) has a higher interaction to CYP 3A4 compared to doxorubicin (-70,52). Both compounds have a similar binding site in Leu 364; Phe 435; Pro 434; Cys 442; Ile 369; Thr 309; dan Ala 305. The overall result of this study showed that PSE has anti-aging effect by decreasing SA- βgal activity. This anti-aging activity possibly due to the interaction between tocopherol to CYP 3A4 based on molecular docking.Keywords: anti-aging, Cucurbita moschata, tocopherol, SA-βgal, molecular docking</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2016-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/103</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev7iss2pp49-53</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 7, No 2 (2016); 49-53</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/103/103</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention</dc:rights>
</oai_dc:dc>