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Ethanolic Extract of Hedyotis corymbosa and Its Combination with 5-FU Inhibit Cyclin D Expression on WiDr Colorectal Cancer Cell

Indonesian Journal of Cancer Chemoprevention · Vol. 7 No. 1 (2016) · hlm. 25–30

DOI
10.14499/indonesianjcanchemoprev7iss1pp25-30
ISSN
2355-8989, 2088-0197
Terbit
2016-02-29
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

Hedyotis corymbosa has been used for long time as an important component in several folklore medicine formula to clinically treat various types of cancer, including colorectal cancer (CRC). Previously, Hedyotis corymbosa ethanolic extract (HEE) which contain ursolic acid reported to inhibit CRC growth via induction of cancer cell apop­tosis and blocked the cell cycle, preventing G1 to S progression where cyclin D highly espressed in this phase. 5-fluorouracil (5FU), the first line chemotherapy of colorectal cancer have had resistence and possessed several side effects such as neutropenia, immunosuppression, diarrhea, and also constipation. Therefore, the aim of this research is to conduct the antiproliferative effect and molecular analysis of HEE and its combination with 5FU. Molecular docking study was also done to approach the specific protein target of the compound. Antiproliferative effect was conducted by MTT assay, while cyclin D expression was examined by immunofluorescence. The proliferative effect showed that both HEE and 5-FU had cytotoxic effect with IC50 value of 65 µg/mL and 90 µM respectively, meanwhile the combination of HEE and 5FU have synergism effect with CI = 0.48 on dose HEE = 22 µg/mL and 5FU= 6.25 µM. Immunofluorescence assay showed HEE and its combination with 5FU suppressed the expression of cyclin D. From molecular docking simulation, ursolic acid performed stable interaction with cyclin D. Our findings suggest that HEE may be an effective treat­ment for co-chemotherapic for 5-FU through inhibition of cyclin D expression.Keywords: Hedyotis corymbosa, 5-fluorouracil, colorectal cancer, WiDr, cyclin D

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  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Sultan Agung Islamic University
  • Sunan Kalijaga State Islamic University Yogyakarta
  • Universitas Wahid Hasyim
  • Padjadjaran University
  • Sebelas Maret University
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Rekan penulisOrganisasiArtikel bersama
Edy MeiyantoUniversitas Gadjah Mada16
Rohmad Yudi UtomoUniversitas Gadjah Mada7
Sri HandayaniIndonesian Institute of Sciences6
Ratna Asmah SusidartiUniversitas Gadjah Mada6
Prisnu TirtanirmalaUniversitas Gadjah Mada4
Adam HermawanUniversitas Gadjah Mada3
Hilyatul FadliyahUniversitas Gadjah Mada3
Meirizky Zulharini S.Universitas Gadjah Mada3
Zalinar UdinIndonesian Institute of Sciences2
Yogi ErtantoUniversitas Gadjah Mada2
Muthi IkawatiUniversitas Gadjah Mada2
Laili Nailul MunaSunan Kalijaga State Islamic University Yogyakarta2
Laeli MuntafiahUniversitas Gadjah Mada2
Bani Adlina ShabrinaUniversitas Gadjah Mada2
Rumiyati RumiyatiUniversitas Gadjah Mada2
Dhania NovitasariUniversitas Gadjah Mada2
Devi Nisa HidayatiUniversitas Wahid Hasyim2
Imroatus SholihahUniversitas Gadjah Mada2
Puspa Dewi LotulungIndonesian Institute of Sciences1
Akhmad DarmawanIndonesian Institute of Sciences1
Rosa AdelinaAfiliasi tidak diketahui1
Maria Dwi SupriyatiAfiliasi tidak diketahui1
Dwi Ana NawangsariAfiliasi tidak diketahui1
Dhiya Ulhaq SalsabilaPadjadjaran University1
Nisa Ul HasanahUniversitas Gadjah Mada1
Ahmad Syauqy TafrihaniUniversitas Gadjah Mada1
Ummi Maryam ZulfinUniversitas Gadjah Mada1
Ratih Kurnia WardaniUniversitas Gadjah Mada1
Agung PutraSultan Agung Islamic University1
Iffan AlifSultan Agung Islamic University1
Dwi SulistyowatiUniversitas Gadjah Mada1
Murah Riski Novi AsshagabUniversitas Gadjah Mada1
Anif Nur ArtantiSebelas Maret University1
Khairunissa IrnandaUniversitas Gadjah Mada1
Standie NagadiUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Ethanolic Extract of Hedyotis corymbosa and Its Combination with 5-FU Inhibit Cyclin D Expression on WiDr Colorectal Cancer Cell</dc:title>
	<dc:creator>Meiftasari, Argandita</dc:creator>
	<dc:creator>W.P., Januar Caesar</dc:creator>
	<dc:creator>Novarina, Annisa</dc:creator>
	<dc:creator>W., Julika Yovi</dc:creator>
	<dc:creator>Jenie, Riris Istighfari</dc:creator>
	<dc:description xml:lang="en-US">Hedyotis corymbosa has been used for long time as an important component in several folklore medicine formula to clinically treat various types of cancer, including colorectal cancer (CRC). Previously, Hedyotis corymbosa ethanolic extract (HEE) which contain ursolic acid reported to inhibit CRC growth via induction of cancer cell apop­tosis and blocked the cell cycle, preventing G1 to S progression where cyclin D highly espressed in this phase. 5-fluorouracil (5FU), the first line chemotherapy of colorectal cancer have had resistence and possessed several side effects such as neutropenia, immunosuppression, diarrhea, and also constipation. Therefore, the aim of this research is to conduct the antiproliferative effect and molecular analysis of HEE and its combination with 5FU. Molecular docking study was also done to approach the specific protein target of the compound. Antiproliferative effect was conducted by MTT assay, while cyclin D expression  was examined by immunofluorescence. The proliferative effect showed that both HEE and 5-FU had cytotoxic effect with IC50 value of 65 µg/mL and 90 µM respectively, meanwhile the combination of HEE and 5FU have synergism effect with CI = 0.48 on dose HEE = 22 µg/mL and 5FU= 6.25 µM. Immunofluorescence assay showed HEE and its combination with 5FU suppressed the expression of cyclin D. From molecular docking simulation, ursolic acid performed stable interaction with cyclin D. Our findings suggest that HEE may be an effective treat­ment for co-chemotherapic for 5-FU through inhibition of cyclin D expression.Keywords : Hedyotis corymbosa, 5-fluorouracil, colorectal cancer,  WiDr, cyclin D </dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2016-02-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/99</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev7iss1pp25-30</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 7, No 1 (2016); 25-30</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/99/99</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention</dc:rights>
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