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Simple Procedure for the Isolation of Mesenchymal Stem Cells from Different Parts of the Human Umbilical Cord

Indonesian Journal of Cancer Chemoprevention · Vol. 13 No. 2 (2022) · hlm. 104–113

DOI
10.14499/indonesianjcanchemoprev13iss2pp104-113
ISSN
2355-8989, 2088-0197
Terbit
2022-06-30
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

The umbilical cord and placenta are both sources of mesenchymal stem cells (MSCs) that are promising for cell-based therapy. Furthermore, compared to other MSCs sources, they are easy to obtain with no invasive procedures. This study presents an adapted method for stem cell isolation from three different parts of the human umbilical cord, including Wharton’s jelly (WJ), cord lining (CL), and cord-placenta junction (CPJ). The isolation consists of sample preparation, tissue dissection into distinct anatomical regions, mincing and enzyme digestion, and explant culturing. In addition, we monitored when the cells migrated from the explant to the bottom of the cell culture dish and passed the cells after they became confluent. This study found that WJ cells were the first to reach confluence at Passage 0 (P0). In contrast, CL cells needed the longest time to get confluence at P0 but displayed faster cell growth after subsequent passages (P1-P2). In addition, CPJ cells showed growth retardation after P1 and P2. Altogether, we could extract the MSCs from umbilical cord tissue explants by using DMEM supplemented with 10% FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin as general cell culture medium and omitting the use of gentamicin. However, the MSCs may need a more complex specified medium for optimum cell regeneration for further cell expansion.Keywords: mesenchymal stem cells, umbilical cord, Wharton’s jelly, cord lining, cord-placenta junction.

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Adi SantosoAfiliasi tidak diketahui11
Popi Hadi WisnuwardhaniAfiliasi tidak diketahui8
Ratna Dwi RamadaniAfiliasi tidak diketahui7
Yana RubiyanaAfiliasi tidak diketahui7
Ratih Asmana NingrumAfiliasi tidak diketahui5
Arizah KusumawatiAfiliasi tidak diketahui3
Ria Fajarwati KastianAfiliasi tidak diketahui3
Khairul AnamUniversitas Jember3
Komang Alit ParamitasariAfiliasi tidak diketahui3
Alayna Lillahida Indri HimawanAfiliasi tidak diketahui3
Salsabila AzzahraAfiliasi tidak diketahui3
Neng HerawatiAfiliasi tidak diketahui3
Neny NurainiAfiliasi tidak diketahui2
Metta NoviantiAfiliasi tidak diketahui2
Wien KusharyotoAfiliasi tidak diketahui2
Dian Fitria AgustiyantiAfiliasi tidak diketahui2
Andri WardianaAfiliasi tidak diketahui2
Gracia Christine Lembong PurwantoAfiliasi tidak diketahui2
Fedric Intan DamaiAfiliasi tidak diketahui2
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Muhammad Khairul Lisan SidqiAfiliasi tidak diketahui2
Dadang SupriatnaAfiliasi tidak diketahui2
Ihsan Tria PramandaAfiliasi tidak diketahui2
Eti YerizelAfiliasi tidak diketahui1
Dita FauziahAfiliasi tidak diketahui1
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Larasati LarasatiIPB University1
Ratih Asma NingrumAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Simple Procedure for the Isolation of Mesenchymal Stem Cells from Different Parts of the Human Umbilical Cord</dc:title>
	<dc:creator>Suyoko, Ahmad</dc:creator>
	<dc:creator>Prasetyaningrum, Pekik Wiji</dc:creator>
	<dc:creator>Septisetyani, Endah Puji</dc:creator>
	<dc:description xml:lang="en-US">The umbilical cord and placenta are both sources of mesenchymal stem cells (MSCs) that are promising for cell-based therapy. Furthermore, compared to other MSCs sources, they are easy to obtain with no invasive procedures. This study presents an adapted method for stem cell isolation from three different parts of the human umbilical cord, including Wharton’s jelly (WJ), cord lining (CL), and cord-placenta junction (CPJ). The isolation consists of sample preparation, tissue dissection into distinct anatomical regions, mincing and enzyme digestion, and explant culturing. In addition, we monitored when the cells migrated from the explant to the bottom of the cell culture dish and passed the cells after they became confluent. This study found that WJ cells were the first to reach confluence at Passage 0 (P0). In contrast, CL cells needed the longest time to get confluence at P0 but displayed faster cell growth after subsequent passages (P1-P2). In addition, CPJ cells showed growth retardation after P1 and P2. Altogether, we could extract the MSCs from umbilical cord tissue explants by using DMEM supplemented with 10% FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin as general cell culture medium and omitting the use of gentamicin. However, the MSCs may need a more complex specified medium for optimum cell regeneration for further cell expansion.Keywords: mesenchymal stem cells, umbilical cord, Wharton’s jelly, cord lining, cord-placenta junction.</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2022-06-30</dc:date>
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	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/410</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev13iss2pp104-113</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 13, No 2 (2022); 104-113</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/410/259</dc:relation>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/downloadSuppFile/410/36</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Indonesian Journal of Cancer Chemoprevention</dc:rights>
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