Melola Discovery

← Melola Discovery

Jurnal SINTA 1

Evaluation of The Genotoxicity of Three Food Additives using CHO-K1 Cells under in vitro Micronucleus Flow Cytometry Assay

Indonesian Journal of Cancer Chemoprevention · Vol. 8 No. 2 (2017) · hlm. 74–80

DOI
10.14499/indonesianjcanchemoprev8iss2pp74-80
ISSN
2355-8989, 2088-0197
Terbit
2017-06-30
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

Exposure of genotoxic substances come from various sources such as food additives. The aim of this study is to evaluate the genotoxicity of food additives in CHO-K1 cells by micronucleus test flow cytometry. The food additives: sodium saccharine (SS), monosodium glutamate (MSG), and sodium benzoate (SB) were assessed by in vitro cytotoxicity and genotoxicity using Chinese Hamster Ovary-K1 (CHO-K1) cells. The cytotoxic effect of those compounds was evaluated by MTT Assay on CHO-K1 Cells. The genotoxic evaluation was observed by in vitro micronucleus test by flowcytometry with double staining method. The results showed that the three compounds did not perform cytotoxic effect, increased the frequency of micronucleus, and changed the cell cycle profiles. In general, these studies obtained that none of three food additives showed cytotoxic and genotoxic effect on CHO-K1 cells. Micronucleus test using flow cytometry is suitable for this purpose study.Key words: food additives, genotoxic, cytotoxic, micronucleus

Jaringan kolaborasi para penulis

40 penulis · 132 tautan kepenulisan bersama · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • University of Brawijaya
  • IPB University
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Adam HermawanAfiliasi tidak diketahui19
Ratna Asmah SusidartiAfiliasi tidak diketahui17
Riris Istighfari JenieAfiliasi tidak diketahui17
Muthi’ IkawatiAfiliasi tidak diketahui11
Rohmad Yudi UtomoAfiliasi tidak diketahui8
Dyaningtyas Dewi Pamungkas PutriAfiliasi tidak diketahui8
Retno MurwantiAfiliasi tidak diketahui8
Sri HandayaniUniversity of Brawijaya5
Suwijiyo PramonoAfiliasi tidak diketahui5
Faaza Aulia RahmanAfiliasi tidak diketahui4
Desty Restia RahmawatiAfiliasi tidak diketahui4
Erlina RivantiAfiliasi tidak diketahui4
Ummi Maryam ZulfinAfiliasi tidak diketahui3
Anindyajati AnindyajatiAfiliasi tidak diketahui3
Larasati LarasatiIPB University3
Sendy JunediAfiliasi tidak diketahui3
Laeli MuntafiahAfiliasi tidak diketahui3
Fajar Oktavian MuljonoAfiliasi tidak diketahui2
Firghi Muhammad FardhanAfiliasi tidak diketahui2
Dian SusantiAfiliasi tidak diketahui2
Yuli WidiyastutiAfiliasi tidak diketahui2
Masashi KawaichiAfiliasi tidak diketahui2
Nunuk Aries NurulitaAfiliasi tidak diketahui2
Zalinar UdinAfiliasi tidak diketahui2
Yogi ErtantoAfiliasi tidak diketahui2
Lailatul QodriaAfiliasi tidak diketahui2
Gergorius Gena MaranAfiliasi tidak diketahui2
Aulia Nur SeptianiAfiliasi tidak diketahui2
Sofa FaridaAfiliasi tidak diketahui2
Asri Mega PutriAfiliasi tidak diketahui2
Hilyatul FadliyahAfiliasi tidak diketahui2
Indri KusharyantiAfiliasi tidak diketahui2
Yonika Arum LarasatiAfiliasi tidak diketahui2
Muhammad Da’iAfiliasi tidak diketahui2

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Evaluation of The Genotoxicity of Three Food Additives using CHO-K1 Cells under in vitro Micronucleus Flow Cytometry Assay</dc:title>
	<dc:creator>Lestari, Beni</dc:creator>
	<dc:creator>Novitasari, Dhania</dc:creator>
	<dc:creator>Putri, Herwandhani</dc:creator>
	<dc:creator>Haryanti, Sari</dc:creator>
	<dc:creator>Sasmito, Ediati</dc:creator>
	<dc:creator>Meiyanto, Edy</dc:creator>
	<dc:description xml:lang="en-US">Exposure of genotoxic substances come from various sources such as food additives. The aim of this study is to evaluate the genotoxicity of food additives in CHO-K1 cells by micronucleus test flow cytometry. The food additives: sodium saccharine (SS), monosodium glutamate (MSG), and sodium benzoate (SB) were assessed by in vitro cytotoxicity and genotoxicity using Chinese Hamster Ovary-K1 (CHO-K1) cells. The cytotoxic effect of those compounds was evaluated by MTT Assay on CHO-K1 Cells. The genotoxic evaluation was observed by in vitro micronucleus test by flowcytometry with double staining method. The results showed that the three compounds did not perform cytotoxic effect, increased the frequency of micronucleus, and changed the cell cycle profiles. In general, these studies obtained that none of three food additives showed cytotoxic and genotoxic effect on CHO-K1 cells. Micronucleus test using flow cytometry is suitable for this purpose study.Key words : food additives, genotoxic, cytotoxic, micronucleus</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2017-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/145</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev8iss2pp74-80</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 8, No 2 (2017); 74-80</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/145/125</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention</dc:rights>
</oai_dc:dc>