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Ficus septica Burm. F. Leaves Ethanolic Extract Induces Apoptosis in 7,12-Dimethylbenz[A]Nthracene-Induced Rat Liver Cancer Quatitavely

Indonesian Journal of Cancer Chemoprevention · Vol. 2 No. 2 (2011) · hlm. 255–260

DOI
10.14499/indonesianjcanchemoprev2iss2pp255-260
ISSN
2355-8989, 2088-0197
Terbit
2011-06-28
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

The chemopreventive effect of Ficus septica Burm. f. leaves ethanolic extract (FLEE) was studied in 7,12-dimethylbenz[a]nthracene(DMBA)-induced rat liver cancer. Rats were divided into 5 group, 5 rats (5 wk of age Sprague Dawley rat) in each group. Group 1 was control diet group, administered with 0,5% CMC-Na as vehicle. FLEE was administered 750 mg/kgBW and 1500 mg/kgBW starting 4 wk until 5 wk after DMBA administration at the first until fifth wk to group 2 and group 3. Group 4 was control extract group, administered with 750 mg/kgBW and group 5 was DMBA group. DMBA is a carcinogen to induce liver cancer was also administered in DMBA control group and all animals were necropsied at 6 wk after DMBA administration. Activity of inducing apoptosis was detected using Double Staining method in 750 mg/kgBW FLEE group compared to control group but no in 1500 mg/kgBW FLEE group resulted in 100% dead. Apoptotic cells would have orange flourescence but normal cells would have green flourescence detected by flourescence microscope. To investigate the protein that involved in apoptotic mechanism, we studied p53 expression using Imunohistochemistry (IHC). There was no difference expression of p53 in both tested and control groups. Based on the results, FLEE has a potency as chemoprentive agent because its activity on inducing apoptosis in liver cancer with p53-independent pathway. The mechanism of apoptosis induction of this extract needs to be explored by observing the expression of related proteins.Keywords: apoptosis, Ficus septica, liver cancer, p53 independent pathway

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  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Kementerian Kesehatan Republik Indonesia
  • Atma Jaya Catholic University of Indonesia
  • Nara Institute of Science and Technology
  • Atma Jaya University Yogyakarta
  • Muhammadiyah University Purwokerto
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Rekan penulisOrganisasiArtikel bersama
Adam HermawanUniversitas Gadjah Mada19
Ratna Asmah SusidartiUniversitas Gadjah Mada17
Riris Istighfari JenieUniversitas Gadjah Mada15
Muthi IkawatiUniversitas Gadjah Mada11
Dyaningtyas Dewi Pamungkas PutriUniversitas Gadjah Mada9
Rohmad Yudi UtomoUniversitas Gadjah Mada8
Faaza Aulia RahmanUniversitas Gadjah Mada5
Sari HaryantiKementerian Kesehatan Republik Indonesia5
Dhania NovitasariUniversitas Gadjah Mada4
Retno MurwantiUniversitas Gadjah Mada4
Desty Restia RahmawatiUniversitas Gadjah Mada4
Erlina RivantiUniversitas Gadjah Mada4
Sri HandayaniIndonesian Institute of Sciences3
Ummi Maryam ZulfinUniversitas Gadjah Mada3
Larasati LarasatiUniversitas Gadjah Mada3
Suwijiyo PramonoUniversitas Gadjah Mada3
Masashi KawaichiNara Institute of Science and Technology2
Sendy JunediAtma Jaya University Yogyakarta2
Nunuk Aries NurulitaMuhammadiyah University Purwokerto2
Zalinar UdinIndonesian Institute of Sciences2
Yogi ErtantoUniversitas Gadjah Mada2
Lailatul QodriaUniversitas Gadjah Mada2
Gergorius Gena MaranUniversitas Gadjah Mada2
Aulia Nur SeptianiUniversitas Gadjah Mada2
Sofa FaridaUniversitas Gadjah Mada2
Beni LestariUniversitas Gadjah Mada2
Asri Mega PutriUniversitas Gadjah Mada2
Hilyatul FadliyahUniversitas Gadjah Mada2
Indri KusharyantiTanjungpura University2
Yonika Arum LarasatiUniversitas Gadjah Mada2
Raditya Prima IstiajiUniversitas Gadjah Mada2
Ni Putu Linda LaksmianiUdayana University2
Nanda Resa PratamaUniversitas Gadjah Mada2
Raymond R. TjandrawinataAtma Jaya Catholic University of Indonesia2

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Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Ficus septica Burm. F. Leaves Ethanolic Extract Induces Apoptosis in 7,12-Dimethylbenz[A]Nthracene-Induced Rat Liver Cancer Quatitavely</dc:title>
	<dc:creator>Septhea, Dita Brenna</dc:creator>
	<dc:creator>Anindyajati, Anindyajati</dc:creator>
	<dc:creator>Darma, Andita Pra</dc:creator>
	<dc:creator>Nurzijah, Ika</dc:creator>
	<dc:creator>Nugroho, Agung Endro</dc:creator>
	<dc:creator>Meiyanto, Edy</dc:creator>
	<dc:description xml:lang="en-US">The chemopreventive effect of Ficus septica Burm. f. leaves ethanolic extract (FLEE) was studied in 7,12-dimethylbenz[a]nthracene(DMBA)-induced rat liver cancer. Rats were divided into 5 group, 5 rats (5 wk of age Sprague Dawley rat) in each group. Group 1 was control diet group, administered with 0,5% CMC-Na as vehicle. FLEE was administered 750 mg/kgBW and 1500 mg/kgBW starting 4 wk until 5 wk after DMBA administration at the first until fifth wk to group 2 and group 3. Group 4 was control extract group, administered  with 750 mg/kgBW and group 5 was DMBA group. DMBA is a carcinogen to induce liver cancer was also administered in DMBA control group and all animals were necropsied at 6 wk after DMBA administration. Activity of inducing apoptosis was detected using Double Staining method in 750 mg/kgBW FLEE group compared to control group but no in 1500 mg/kgBW FLEE group resulted in 100% dead. Apoptotic cells would have orange flourescence but normal cells would have green flourescence detected by flourescence microscope. To investigate the protein that involved in apoptotic mechanism, we studied p53 expression using Imunohistochemistry (IHC). There was no difference expression of p53 in both tested and control groups. Based on the results, FLEE has a potency as chemoprentive agent because its activity on inducing apoptosis in liver cancer with p53-independent pathway. The mechanism of apoptosis induction of this extract needs to be explored by observing the expression of related proteins.Keywords: apoptosis, Ficus septica, liver cancer, p53 independent pathway</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2011-06-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/39</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev2iss2pp255-260</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 2, No 2 (2011); 255-260</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/39/40</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention</dc:rights>
</oai_dc:dc>