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Red Betel Leaves Methanolic Extract (Piper crocatum Ruiz & Pav.) Increases Cytotoxic Effect of Doxorubicin on WiDr Colon Cancer Cells through Apoptosis Induction

Indonesian Journal of Cancer Chemoprevention · Vol. 9 No. 1 (2018) · hlm. 1–8

DOI
10.14499/indonesianjcanchemoprev9iss1pp1-8
ISSN
2355-8989, 2088-0197
Terbit
2018-02-28
Penerbit
Indonesian Society for Cancer Chemoprevention
Bahasa
eng

Abstrak

Doxorubicin is a chemotherapeutic agent that causes a lot of side effects in high doses. Thus, combination with a co-chemotherapeutic agent which can increase its toxicity on cancer cells, is needed to reduce its therapeutic dose. Red betel leaves (Piper crocatum Ruiz & Pav.) have been known to contain flavonoids and alkaloids that have anticancer activity. This study was conducted to determine the cytotoxic effect and apoptosis induction of red betel leaves methanolic extract (RBM), doxorubicin (dox) and the combination of them on WiDr cells as model of colon cancer. RBM was extracted by soxhlet method using methanol. Cytotoxicity assay was performed using MTT assay for both single and combination treatments for 24 hours to determine IC50 and CI as their parameters. Apoptosis induction was analyzed by double staining method using ethidium bromide-acridine orange staining. Treatment of RBM and dox on WiDr cells for 24 hours showed cytotoxic activity with IC50 100 μg/mL and 1.6 μM respectively. Combination of RBM and dox performed synergism effect with CI<0.9 (p<0.05). Combination of RBM (12.5 μg/mL) and dox (0.4 μM) increased the number of apoptosis cells compared to each single treatment. Based on this study, it can be concluded that red betel leaves methanolic extract is potential to be developed as a co-chemotherapeutic agent of doxorubicin on colon cancer but still need further study to disclose the underlying molecular mechanisms.Keywords: Red betel leaves (Piper crocatum Ruiz & Pav.), doxorubicin, WiDr cells, co- chemotherapeutic agent

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  • Universitas Gadjah Mada
  • Indonesian Institute of Sciences
  • Sultan Agung Islamic University
  • Sunan Kalijaga State Islamic University Yogyakarta
  • Universitas Wahid Hasyim
  • Padjadjaran University
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Rekan penulisOrganisasiArtikel bersama
Edy MeiyantoUniversitas Gadjah Mada17
Ratna Asmah SusidartiUniversitas Gadjah Mada7
Sri HandayaniIndonesian Institute of Sciences6
Rohmad Yudi UtomoUniversitas Gadjah Mada5
Annisa NovarinaUniversitas Gadjah Mada4
Adam HermawanUniversitas Gadjah Mada3
Prisnu TirtanirmalaUniversitas Gadjah Mada3
Meirizky Zulharini S.Universitas Gadjah Mada3
Zalinar UdinIndonesian Institute of Sciences2
Yogi ErtantoUniversitas Gadjah Mada2
Muthi IkawatiUniversitas Gadjah Mada2
Laili Nailul MunaSunan Kalijaga State Islamic University Yogyakarta2
Laeli MuntafiahUniversitas Gadjah Mada2
Bani Adlina ShabrinaUniversitas Gadjah Mada2
Rumiyati RumiyatiUniversitas Gadjah Mada2
Dhania NovitasariUniversitas Gadjah Mada2
Devi Nisa HidayatiUniversitas Wahid Hasyim2
Imroatus SholihahUniversitas Gadjah Mada2
Retno MurwantiUniversitas Gadjah Mada2
Yuni Fajar EstiUniversitas Gadjah Mada2
Marsya Yonna NurrachmaUniversitas Gadjah Mada2
Puspa Dewi LotulungIndonesian Institute of Sciences1
Akhmad DarmawanIndonesian Institute of Sciences1
Rosa AdelinaAfiliasi tidak diketahui1
Maria Dwi SupriyatiAfiliasi tidak diketahui1
Dwi Ana NawangsariAfiliasi tidak diketahui1
Dhiya Ulhaq SalsabilaPadjadjaran University1
Nisa Ul HasanahUniversitas Gadjah Mada1
Ahmad Syauqy TafrihaniUniversitas Gadjah Mada1
Ummi Maryam ZulfinUniversitas Gadjah Mada1
Ratih Kurnia WardaniUniversitas Gadjah Mada1
Agung PutraSultan Agung Islamic University1
Iffan AlifSultan Agung Islamic University1
Yoce ApriantoUniversitas Gadjah Mada1
Asri Mega PutriUniversitas Gadjah Mada1
Dwi SulistyowatiUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Red Betel Leaves Methanolic Extract (Piper crocatum Ruiz &amp; Pav.) Increases Cytotoxic Effect of Doxorubicin on WiDr Colon Cancer Cells through Apoptosis Induction</dc:title>
	<dc:creator>Wulandari, Nindi</dc:creator>
	<dc:creator>Meiftasari, Argandita</dc:creator>
	<dc:creator>Fadliyah, Hilyatul</dc:creator>
	<dc:creator>Jenie, Riris Istighfari</dc:creator>
	<dc:description xml:lang="en-US">Doxorubicin is a chemotherapeutic agent that causes a lot of side effects in high doses. Thus, combination with a co-chemotherapeutic agent which can increase its toxicity on cancer cells, is needed to reduce its therapeutic dose. Red betel leaves (Piper crocatum Ruiz &amp; Pav.) have been known to contain flavonoids and alkaloids that have anticancer activity. This study was conducted to determine the cytotoxic effect and apoptosis induction of red betel leaves methanolic extract (RBM), doxorubicin (dox) and the combination of them on WiDr cells as model of colon cancer. RBM was extracted by soxhlet method using methanol. Cytotoxicity assay was performed using MTT assay for both single and combination treatments for 24 hours to determine IC50 and CI as their parameters. Apoptosis induction was analyzed by double staining method using ethidium bromide-acridine orange staining. Treatment of RBM and dox on WiDr cells for 24 hours showed cytotoxic activity with IC50 100 μg/mL and 1.6 μM respectively. Combination of RBM and dox performed synergism effect with CI&lt;0.9 (p&lt;0.05). Combination of RBM (12.5 μg/mL) and dox (0.4 μM) increased the number of apoptosis cells compared to each single treatment. Based on this study, it can be concluded that red betel leaves methanolic extract is potential to be developed as a co-chemotherapeutic agent of doxorubicin on colon cancer but still need further study to disclose the underlying molecular mechanisms.Keywords :  Red betel leaves (Piper crocatum Ruiz &amp; Pav.), doxorubicin, WiDr cells, co- chemotherapeutic agent</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2018-02-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/197</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev9iss1pp1-8</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 9, No 1 (2018); 1-8</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/197/153</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 Nindi Wulandari, Argandita Meiftasari, Hilyatul Fadliyah, Riris Istighfari Jenie</dc:rights>
</oai_dc:dc>